2010 Fiscal Year Final Research Report
Regulation mechamisms and physiological significance of thermosensitive TRP channels
| Project Area | Molecular interaction and modal shift of cellular sensors |
|---|
| Project/Area Number |
18077012
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
|
| Allocation Type | Single-year Grants |
|---|
| Review Section |
Biological Sciences
|
|---|
| Research Institution | 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設) (2010)
National Institutes of Natural Sciences Okazaki Research Facilities (2006-2009) |
|---|
Principal Investigator |
TOMINAGA Makoto 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (90260041)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
FUKUMI Tomoko 生理学研究所, 細胞器官研究系, 准教授 (00280587)
INADA Hitoshi 大学共同利用機関法人自然科学研究機構, 岡崎共通研究施設, 特任助教 (60419893)
SOKABE Takaaki 大学共同利用機関法人自然科学研究機構, 岡崎共通研究施設, 特別協力研究員 (70419894)
|
|---|
| Project Period (FY) |
2006 – 2010
|
| Keywords | 温度 / TRPチャネル |
|---|
| Research Abstract |
(1) We found that TRPV4 in hippocampal pyramidal neurons is activated at body temperature and involved in the formation of resting membrane potential which could regulate neuronal excitability. (2) We found that TRPV4 in bladder epithelial cells detects wall extension under body temperature and that the Ca^<2+> influx through TRPV4 activation causes ATP release through which the stretch information is transmitted to sensory neurons. (3) We found that mainly TRPV3 detect ambient temperature in skin keratinocytes and that the temperature information is transmitted to sensory neurons through ATP. (4) Single particle analysis with cryoEM revealed 3-demensional structure of TRPV4 at 3.5 nm level. (5) Activation of TRPV4 in skin keratinocytes causes Ca^<2+> influx, strengthening the junctional structure of adherence and tight junctions, which could cause enhancement of the skin barrier functions. (6) We cloned western clawed-frog TRPV3 gene and found that western clawed-frog TRPV3 is activated by cold temperature bellow 17 degree C, which is quite different from the warm temperature activation of mammalian TRPV3.
|
-
[Journal Article] Importance of transient receptor potential vanilloid 4 (TRPV4) in epidermal barrier function in human skin keratinocytes2012
Author(s)
Kida N, Sokabe T, Kashio M, Haruna K, Mizuno Y, Suga Y, Nishikawa K, Kanamaru A, Hongo M, Oba A, Tominaga M.
-
Journal Title
Pfluger Archiv. Eur. J. Physiol.
Volume: 463
Pages: 715-25
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
