Regulatory mechanism for interaction between membrane proton sensor and cytosolic ion sensor

2008 Fiscal Year Self-evaluation Report

• Project Area: Molecular interaction and modal shift of cellular sensors
• Project/Area Number: 18077015
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: WAKABAYASHI Shigeo National Cardiovascular Center Research Institute, 循環分子生理部, 部長 (70158583)
• Project Period (FY): 2006 – 2010
• Keywords: 生理学 / 蛋白質 / 循環器・高血圧 / シグナル伝達 / バイオテクノロジー

Research Abstract

本研究は膜プロトンセンサーであるNa^+/H^+交換輸送体(NHE)が細胞内イオンセンサー蛋白質との相互作用によって生理機能を発揮する機構を明らかにすることを主眼とする。また、関連の深いセンサー蛋白質(NCS-1, CHP, TRPV2)の新しい生理機能を見出すことも視野に入れる。

Research Products

• [Journal Article] Dominant-negative inhibition of Ca^<2+> influx via TRPV2 ameliorates muscular dystrophy in animal models. (2009)
  • Author(s): Iwata, Y., Katanosaka, Y., Arai, Y., Shigekawa, M., Wakabayashi, S.
  • Journal Title: Hum. Mol. Gen 18(5) Pages: 824-834
  • Peer Reviewed
• [Journal Article] Activation of Na^+/H^+ exchanger 1 is sufficient to generate Ca^<2+> signals that induce cardiac hypertrophy and heart failure. (2008)
  • Author(s): Nakamura, T.Y., Iwata, Y., Arai, Y., Komamura, K., Wakabayashi, S.
  • Journal Title: Circ. Res 103(8) Pages: 891-899
  • Peer Reviewed
• [Journal Article] Dimerization is crucial for the function of the Na^+/H^+ exchanger NHE1. (2006)
  • Author(s): Hisamitsu, T., Ben Ammar, Y., Nakamura, T.Y., Wakabayashi, S.
  • Journal Title: Biochemistry 45(44) Pages: 13346-13355
  • Peer Reviewed
• [Journal Article] Crystal structure of CHP2 complexed with NHE1-cytosolic region and an implication for pH regulation. (2006)
  • Author(s): Ben Ammar, Y., Takeda, S., Hisamitsu, T., Mori. H., Wakabayashi, S
  • Journal Title: EMBO J 25(11) Pages: 2315-2325
  • Peer Reviewed
• [Presentation] 新しい心機能調節因子としてのCa^<2+>センサータンパク質NCS-1の役割 (2008)
  • Author(s): 中村(西谷)友重、Jeromin, A., 若林繁夫
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 2008-12-09
• [Presentation] Structural and functional aspects of the Na^+/H^+ exchanger 1 and its obligatory binding partner CHP. (2007)
  • Author(s): Wakabayashi, S.
  • Organizer: Experimental Biology Annual Meeting 2007
  • Place of Presentation: Washington, DC, USA
  • Year and Date: 20070428-20070502
• [Presentation] Novel roles of NCS-1, a calcium sensor protein, on neuronal survival and cardiac excitability. (2006)
  • Author(s): Nakamura, T., Jeromin, A., Nabekura, J., Wakabayashi, S.
  • Organizer: 20^<th> IUBMB International Congress of Biochemistry and Molecular Biology and 11^<th> FAOBMB congress
  • Place of Presentation: Kyoto Japan
  • Year and Date: 20060618-20060623
• [Presentation] Na^+/H^+ exchange inhibitors protect against muscle degerneration in cardiomyopathic hamsters. 26th Annual Scientific Sessions (2006)
  • Author(s): Iwata, Y., Katanosaka, Y., Wakabayashi, S.
  • Organizer: European Section of the ISHR
  • Place of Presentation: Univ. of Manchester, UK
  • Year and Date: 20060614-17
• [Book] 機能調節と薬物標的としての意義(創薬研究者必見!-最新トランスポーター研究2009 "遺伝子医学MOOK12") (2009)
  • Author(s): 中村(西谷)友重、古林創史、久光 隆、岩田裕子、若林繁夫
  • Total Pages: 255-261
  • Publisher: (株)メディカル ドゥ
• [Book] Na^+/H^+交換輸送体の構造・機能と病態的意義循環器病研究の進歩(通巻47号) Vol.XXVIII No.1(2007.11) (2007)
  • Author(s): 若林繁夫、岩田裕子、中村(西谷)友重、久光 隆、ベンアマー・ヨセフ
  • Total Pages: 73-82
  • Publisher: 協和企画
• [Book] 動物細胞Na^+/H^+交換輸送体:分子から疾患まで(2007) 生化学79(6) (2007)
  • Author(s): 若林繁夫、久光 隆、ユセフ・ベンアマー、中村(西谷)友重、岩田裕子
  • Total Pages: 579-587
• [Patent(Industrial Property Rights)] トランスジェニック非ヒト動物 (2007)
  • Inventor(s): 岩田裕子、西谷友重、若林繁夫
  • Industrial Property Rights Holder: (財)ヒューマンサイエンス振興財団
  • Industrial Property Number: 2007-123514
  • Filing Date: 2007-05-08
• [Patent(Industrial Property Rights)] 目的物質精製用タグおよび精製用タグを用いた目的物質の精製方法 (2007)
  • Inventor(s): 若林繁夫
  • Industrial Property Rights Holder: (財)ヒューマンサイエンス振興財団
  • Industrial Property Number: 2007-130239
  • Filing Date: 2007-05-16