2022 Fiscal Year Final Research Report
Elucidation of chromatin regulation by nuclear RNA bodies
Project Area | Chromatin potential for gene regulation |
Project/Area Number |
18H05531
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Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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Allocation Type | Single-year Grants |
Review Section |
Biological Sciences
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Research Institution | Japanese Foundation for Cancer Research |
Principal Investigator |
SAITOH Noriko 公益財団法人がん研究会, がん研究所 がん生物部, 部長 (40398235)
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Co-Investigator(Kenkyū-buntansha) |
落合 博 広島大学, 統合生命科学研究科(理), 准教授 (60640753)
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Project Period (FY) |
2018-06-29 – 2023-03-31
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Keywords | RNA ボデイ |
Outline of Final Research Achievements |
In the cell nucleus, many RNA bodies are formed based on non-coding RNAs that are act as "seeds." This study revealed the molecular mechanisms of the ELEANOR RNA cloud formed in the nucleus of hormone-dependent breast cancer cells. It also focused on the nucleolus which is the largest nuclear RNA body. Specifically, we have elucidated that, in breast cancer, the ELEANOR non-coding RNAs regulate transcription of multiple genes within the ELEANOR-TAD (topologically associated domains) that is a mega base-scale chromatin domain. ELEANOR also mediates long-range chromatin interactions to equilibrate between cancer cell proliferation and apoptosis.
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Free Research Field |
分子細胞生物学、腫瘍医学
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Academic Significance and Societal Importance of the Research Achievements |
ゲノムDNA中、タンパク質をコードするのは2%以下で、mRNAは2万程度にとどまる。その一方、ゲノムのほとんどの領域が転写され、細胞内に存在するノンコーディングRNAは10万にものぼると見積もられている。その多くは発生、分化段階、あるいは疾患特異的に発現し、核内でクロマチンに結合して遺伝子の転写制御に関わる。本研究は未解明なことが多いゲノムのノンコーディング領域の潜在的な重要性の解明に迫り、高次クロマチン構造の制御、さらに乳がんにおける意義を明らかにした。
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