Non-invasive prediction of gamete integrity

2023 Fiscal Year Final Research Report

• Project Area: Ensuring integrity in gametogenesis
• Project/Area Number: 18H05550
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: University of Tsukuba
• Principal Investigator: Yawata Yutaka 筑波大学, 生命環境系, 准教授 (10586457)
• Project Period (FY): 2018-06-29 – 2023-03-31
• Keywords: 配偶子 / 自家蛍光 / CRIF / 共焦点顕微鏡 / 機械学習

Outline of Final Research Achievements

１）Fertility prediction for mouse egges：A population of mouse eggs, regardless of grown under in vivo or in vitro conditions, contains infertile individuals. In the present study, we developed a non-inavasive method to detect the infertile eggs by innate fluorescence signature analysis. 2)Tag-free 3D visualization of mouse testis : A mouse testis consists of germ cells and feeder cells. In the present study, we found the innate fluorescence signature characteristic to each cell types and developed a tag-free 3D visualization of testis structure based on innate fluorescence. ３）Tag-free cell sorting technology: We developed a tag-free method to enrich specific types of cell from a mixed population using a combination of single-cell innate fluoresce analysis and cell-sorting technology.

Free Research Field

一細胞生態学

Academic Significance and Societal Importance of the Research Achievements

１）マウス卵子の受精能予測については、受精能のない卵子を非破壊的に見分けることで、将来的に体外受精効率の向上に資する可能性がある。2)マウス精巣の構造可視化については、手を加えない精巣組織構造の解析が可能となることで、精巣の機能や発達過程の理解に資することが期待される。３）魚類生殖細胞の分取では、代理親魚技術などの改善に役立つことが期待できるだけでなく、非モデル生物における細胞分取技術を確立したところに意義があるといえる。