2011 Fiscal Year Final Research Report
Measurement of organic metabolism using low-energy photons
| Project Area | Molecular Science for Supra Functional Systems ? Development of Advanced Methods for Exploring Elementary Process |
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| Project/Area Number |
19056003
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
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| Research Institution | Osaka University |
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Principal Investigator |
AWAZU Kunio 大阪大学, 大学院・工学研究科, 教授 (30324817)
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| Co-Investigator(Kenkyū-buntansha) |
HAZAMA Hisanao 大阪大学, 大学院・工学研究科, 助教 (70437375)
ISHII Katsunori 大阪大学, 大学院・工学研究科, 助教 (20512073)
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| Project Period (FY) |
2007 – 2011
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| Keywords | 質量分析 / タンパク質 / イオン化メカニズム / 中赤外レーザー |
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| Research Abstract |
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is widely used in the analysis of biomolecules such as proteins. However, the mechanism of MALDI has not been fully understood. If the MALDI process is elucidated and controlled, further progress in the biomedical fields is expected. In this study, we have used a mid-infrared laser instead of a conventional ultraviolet laser for MALDI and have succeeded in ionizing an insoluble analyte which has been hard to ionize. In addition, we have found that the wavelength dependence of the ionization efficiency of peptides is correlated with the chemical structure of the matrix and that the water solvent has influence on the ionization with MALDI.
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[Presentation] Development of a stigmatic imaging mass spectrometer using laser desorption/ionization2011
Author(s)
Kunio Awazu, Hisanao Hazama, Hirofumi Nagao, Hidetoshi Yoshimura, Jun Aoki, Kenichi Fuji, Katsuyoshi Masuda, Toshio Tashima, Michisato Toyoda, Yasuhide Naito
Organizer
SPIE Photonics West, BiOS 2011
Place of Presentation
San Francisco, CA, USA
Year and Date
20110122-27
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[Presentation] Observation of tissue sections using a stigmatic mass microscope with a multi-turn time-of-flight mass spectrometer2011
Author(s)
Hisanao Hazama, Hidetoshi Yoshimura, Jun Aoki, Hirofumi Nagao, Yasuhide Naito, Michisato Toyoda, Katsuyoshi Masuda, Kenichi Fujii, Toshio Tashima, Kunio Awazu
Organizer
59th ASMS Conference on Mass Spectrometry and Allied Topics
Place of Presentation
Denver, CO, USA
Year and Date
2011-06-07
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[Presentation] Development of a stigmatic mass microscope with laser ionization and a multi-turn time-of-flight mass spectrometer2010
Author(s)
Hisanao Hazama, Hidetoshi Yoshimura, Jun Aoki, Hirofumi Nagao, Yasuhide Naito, Michisato Toyoda, Katsuyoshi Masuda, Kenichi Fujii, Toshio Tashima, Kunio Awazu
Organizer
Saratov Fall Meeting
Place of Presentation
Saratov, Russia(Invited)
Year and Date
20101005-08
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[Presentation] Observation of tissue sections stained with dyes using a stigmatic imaging mass spectrometer2010
Author(s)
Hisanao Hazama, Hidetoshi Yoshimura, Jun Aoki, Hirofumi Nagao, Yasuhide Naito, Michisato Toyoda, Katsuyoshi Masuda, Kenichi Fujii, Toshio Tashima, Kunio Awazu
Organizer
58th ASMS Conference on Mass Spectrometry and Allied Topics
Place of Presentation
Salt Lake City, UT, USA
Year and Date
20100523-27
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