2011 Fiscal Year Final Research Report
Study of membrane binding protein complex using total internal reflection fluorescence correlation spectroscopy
| Project Area | Protein community: organization and maintenance of protein functions |
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| Project/Area Number |
19058001
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Hokkaido University |
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Principal Investigator |
KINJO Masataka 北海道大学, 大学院・先端生命科学研究院, 教授 (70177971)
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| Co-Investigator(Kenkyū-buntansha) |
MIKUNI Shintaro 北海道大学, 大学院・医学研究科, 特任助教 (40435954)
KITAMURA Akira 北海道大学, 大学院・先端生命科学研究院, 助教 (10580152)
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| Project Period (FY) |
2007 – 2011
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| Keywords | 生物物理 / 生体分子 / 蛍光測定 / 分子集合体 / 一分子検出 / 凝集タンパク質 |
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| Research Abstract |
The functional molecules move around in the cell and interact with other molecules, and then support higher function of the cell. Moreover, even aggregate prone protein acts as aggregate and disaggregate under specific condition, so that each element of these aggregate protein is exchanged with free its molecules in solution. FCS (fluorescence correlation spectroscopy) detects the fluorescence fluctuations, the diffusion coefficient, molecular concentration and molecular interactions of molecules. We developed a multipoint FCS system which was based on an objective-type total internal reflection-FCS (TIR-FCS) in order to analysis molecular interaction in living cell and system of protein community.
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