Elucidation of the yeast systems that achieve and maintain functions and organizations of mitochondrial and ER proteins

2011 Fiscal Year Final Research Report

• Project Area: Protein community: organization and maintenance of protein functions
• Project/Area Number: 19058005
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Nagoya University
• Principal Investigator: ENDO Toshiya 名古屋大学, 理学研究科, 教授 (70152014)
• Co-Investigator(Kenkyū-buntansha): NISHIKAWA Shuh-ichi 名古屋大学, 大学院・理学研究科, 准教授 (10252222)
• Co-Investigator(Renkei-kenkyūsha): YOSHIHIA Tohru 名古屋大学, 物質科学国際研究センター, 准教授 (60212312)
• Project Period (FY): 2007 – 2011
• Keywords: ミトコンドリア / 酵母 / トランスロケータ / 小胞体 / 膜透過 / 品質管理

Research Abstract

We have revealed the mechanisms of various aspects of protein transport into mitochondria, including cooperation of translocators, driving force for protein translocation, roles of multiple targeting signals in the presequence, mapping of subunit arrangements in the translocator complex, assembly of β-barrel proteins etc. We have also revealed the mechanisms of protein quality control in the ER, including roles of interactions between Mnl1 and PDI, ER retention of aberrant proteins by Yos9, roles of Hsp70 in plant sexual reproduction etc.

Research Products

• [Journal Article] Phosphatidylethanolamine biogenesis in mitochondria: phosphatidylserine (PS) trafficking is independent of a PS decarboxylase and intermembrane space proteins, Ups1p and Ups2p (2012)
  • Author(s): Y. Tamura, O. Onguka, K. Itoh, T. Endo, M. Iijima, S. M. Claypool, H. Sesaki
  • Journal Title: J. Biol. Chem Volume: 287 Pages: 43961-43971
  • DOI: doi:10.1074/jbc.M112.390997
  • Peer Reviewed
• [Journal Article] The Tom40 assembly process probedusing the attachment of different intra-mitochondrial sorting signals (2012)
  • Author(s): T. Shiota, M. Maruyama, M. Miura, Y. Tamura, K. Yamano, M. Esaki, and T. Endo
  • Journal Title: Mol. Biol. Cell Volume: 23 Pages: 3936-3947
  • DOI: doi:10.1016/j.celrep.2012.08.010
  • Peer Reviewed
• [Journal Article] Role of Dom34:Hbs1 in non-stop protein clearance for normal organelle protein influx (2012)
  • Author(s): T. Izawa, T. Tsuboi, K. Kuroha, T. Inada, S.Nishikawa, and T. Endo
  • Journal Title: Cell Reports Volume: 2 Pages: 447-453
  • DOI: doi:10.1016/j.celrep.2012.08.010
  • Peer Reviewed
• [Journal Article] Yos9p and Hrd1p mediate ER retention of misfolded proteins for ER-associated degradation (2012)
  • Author(s): T. Izawa, H. Nagai, T. Endo, and S. Nishikawa
  • Journal Title: Mol. Biol. Cell Volume: 23 Pages: 1283-1293
  • DOI: doi:10.1091/mbc.E11-08-0722
  • Peer Reviewed
• [Journal Article] In vivo protein-interaction mapping of a mitochondrial translocator protein Tom22 atwork (2011)
  • Author(s): T. Shiota, H. Mabuchi, S. Tanaka-Yamano, K.Yamano, and T. Endo
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 108 Pages: 15179-15183
  • DOI: doi:10.1073/pnas.1105921108
  • Peer Reviewed
• [Journal Article] Structural insight into the mitochondrial protein import system (2011)
  • Author(s): T. Endo, K. Yamano, and S. Kawano
  • Journal Title: Biochim. Biophys. Acta (Biomembranes) Volume: 1808 Pages: 955-970
  • DOI: doi:10.1016/j.bbamem.2010.07.018
  • Peer Reviewed
• [Journal Article] BamE structure: the assembly of β -barrel proteins in the outer membranes of bacteria and mitochondria (2011)
  • Author(s): T. Endo, S. Kawano, and K. Yamano
  • Journal Title: EMBO Rep. (hot off the press) Volume: 12 Pages: 94-95
  • DOI: doi:10.1038/embor. 2010.217
  • Peer Reviewed
• [Journal Article] TIM23-mediatedinsertion of transmembrane α-helices into the mitochondrial inner membrane (2011)
  • Author(s): S.C. Botelho, M. Osterberg, A.S. Reichert, K.Yamano, P. Bjorkholm, T. Endo, G. von Heijne, and H. Kim
  • Journal Title: EMBO J Volume: 30 Pages: 1003-1011
  • DOI: doi:10.1038/emboj.2011.29
  • Peer Reviewed
• [Journal Article] Dual role of the receptor Tom20in specificity and efficiency of protein import into mitochondria (2011)
  • Author(s): H. Yamamoto, N. Itoh, S. Kawano, Y. Yatsukawa, T. Momose, T. Makio, M. Matsunaga, M. Yokota, M. Esaki, T. Shodai, D. Kohda, A.E.A. Hobbs, R.E. Jensen, and T.Endo
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 108 Pages: 91-96
  • DOI: doi:10.1073/pnas.1014918108
  • Peer Reviewed
• [Journal Article] Tom7 regulates Mdm10-mediated assembly of the mitochondrial import channelprotein Tom40 (2010)
  • Author(s): K. Yamano, S. Tanaka-Yamano, and T. Endo
  • Journal Title: J. Biol. Chem Volume: 285 Pages: 41222-41231
  • DOI: doi:10.1074/jbc.M110.163238
  • Peer Reviewed
• [Journal Article] Dual functions of yeasttRNA ligase in the unfolded protein response: unconventional cytoplasmic splicing of HAC1 pre-mRNA is not sufficient to releasetranslational attenuation (2010)
  • Author(s): T. Mori, C. Ogasawara, T. Inada, M. Englert, H. Beier, M. Takezawa, T. Endo, and T. Yoshihisa
  • Journal Title: Mol. Biol. Cell Volume: 21 Pages: 3722-3734
  • Peer Reviewed
• [Journal Article] Mitochondrial matrix reloaded with RNA (2010)
  • Author(s): T. Endo, K. Yamano, and T. Yoshihisa
  • Journal Title: Cell (Preview) Volume: 142 Pages: 362-363
  • DOI: doi:10.1016/j.cell.2010.07.024
  • Peer Reviewed
• [Journal Article] Structural basis for the disulfide relay systemin the mitochondrial intermembrane space (2010)
  • Author(s): T. Endo, K. Yamano, and S. Kawano
  • Journal Title: Antioxid. Redox Signal Volume: 13 Pages: 1359-1373
  • Peer Reviewed
• [Journal Article] On the impact of precursor unfolding during protein import into chloroplasts (2010)
  • Author(s): M. Ruprecht, T. Bionda, T. Sato, M.S. Sommer, T. Endo, and E. Schleiff
  • Journal Title: Mol. Plant Volume: 3 Pages: 499-508
  • DOI: doi:10.1093/mp/ssp116
  • Peer Reviewed
• [Journal Article] A vacuolarcarboxypeptidase mutant of Arabidopsis thaliana is degraded by the ERAD pathwayindependently of its N-glycan (2010)
  • Author(s): M. Yamamoto, M. Kawanabe, Y. Hayashi, T.Endo, and S. Nishikawa
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 393 Pages: 384-389
  • DOI: doi:10.1016/j.bbrc.2010.02.001
  • Peer Reviewed
• [Journal Article] Structural basis for unfolding pathway-dependent stability of proteins: vectorial unfolding vs. global unfolding (2010)
  • Author(s): K. Yagawa, K. Yamano, T. Oguro, M. Maeda, T. Sato, T. Momose, S. Kawano, and T. Endo
  • Journal Title: Protein Science Volume: 19 Pages: 693-702
  • DOI: doi:10.1002/pro.346
  • Peer Reviewed
• [Journal Article] Identification of yeast Art5 as a multicopysuppressor for the mitochondrial translocatormaintenance protein Tam41 (2010)
  • Author(s): Y. Harada, Y. Tamura, and T. Endo
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 392 Pages: 228-233
  • DOI: doi:10.1016/j.bbrc.2010.01.024
  • Peer Reviewed
• [Journal Article] Mdm10 as a dynamic constituent ofthe TOB/SAM complex directs coordinatedassembly of Tom40 (2010)
  • Author(s): K. Yamano, S. Tanaka-Yamano, and T. Endo
  • Journal Title: EMBO Rep Volume: 11 Pages: 187-193
  • DOI: doi:10.1038/embor.2009.283
  • Peer Reviewed
• [Journal Article] Bip-mediated polar nuclei fusion isessential for the regulation of endosperm nuclei proliferation in Arabidopsis thaliana (2010)
  • Author(s): D. Maruyama, T. Endo, and S. Nishikawa
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 107 Pages: 1684-1689
  • DOI: doi:10.1073/pnas.0905795107
  • Peer Reviewed
• [Journal Article] Transport ofproteins across or into the mitochondrial outermembrane (2010)
  • Author(s): T. Endo and K. Yamano
  • Journal Title: Biochim. Biophys. Acta (Molecular Cell Research) Volume: 1803 Pages: 706-714
  • DOI: doi:10.1016/j.bbamcr.2009.11.007
  • Peer Reviewed
• [Journal Article] Roles of TOM70 in import of presequence-containing mitochondrial proteins (2009)
  • Author(s): H. Yamamoto, K. Fukui, H. Takahashi, S.Kitamura, T. Shiota, K. Terao, M. Uchida, M.Esaki, S. Nishikawa, T. Yoshihisa, K. Yamano, and T. Endo
  • Journal Title: J. Biol. Chem Volume: 284 Pages: 31635-31646
  • DOI: doi:10.1074/jbc.M109.041756
  • Peer Reviewed
• [Journal Article] Structural basis of yeast Tim40 as an oxidative translocator in the mitochondrial intermembrane space (2009)
  • Author(s): S. Kawano, K. Yamano, M. Naoe, T. Momose, K. Terao, S. Nishikawa, N. Watanabe, and T. Endo
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 106 Pages: 14403-14407
  • DOI: doi:10.1073/pnas.0901793106
  • Peer Reviewed
• [Journal Article] Multiplepathways for mitochondrial protein traffic (2009)
  • Author(s): T. Endo and K. Yamano
  • Journal Title: Biol. Chem Volume: 390 Pages: 723-730
  • DOI: doi:10.1515/ BC.2009.087
  • Peer Reviewed
• [Journal Article] Ups1p and Ups2p antagonistically regulate cardiolipin metabolism in mitochondria (2009)
  • Author(s): Y. Tamura, T. Endo, M. Iijima, and H. Sesaki
  • Journal Title: J. Cell Biol Volume: 185 Pages: 1029-1045
  • DOI: doi:10.1083/jcb.200812018
  • Peer Reviewed
• [Journal Article] Roles of protein disulfide isomerase-mediated disulfide bond formation of yeast Mnllp in ER-associated degradation. (2009)
  • Author(s): M. Sakoh-Nakatogawa, S. Nishikawa, and T.Endo
  • Journal Title: J. Biol. Chem Volume: 284 Pages: 11815-11825
  • DOI: doi:10.1074/jbc.M900813200
  • Peer Reviewed
• [Journal Article] Tim23-Tim50 pair coordinates functions of translocators and motor proteins in mitochondrial protein import (2009)
  • Author(s): Y. Tamura, Y. Harada, T. Shiota, K. Yamano, K. Watanabe, M. Yokota, H. Yamamoto, H. Sesaki, and T. Endo
  • Journal Title: J. Cell Biol Volume: 184 Pages: 129-141
  • DOI: doi:10.1083/jcb.200808068
  • Peer Reviewed
• [Journal Article] Structural stabilities of different regions of the titin I27 domain contribute differently to unfolding upon mitochondrial protein import (2009)
  • Author(s): T. Oguro, K. Yagawa, T. Momose, T. Sato, K. Yamano, and T. Endo
  • Journal Title: J. Mol. Biol Volume: 385 Pages: 811-819
  • DOI: doi:10.1016/j.jmb.2008.10.076
  • Peer Reviewed
• [Journal Article] Nuclear inner membrane fusion facilitated byyeast Jem1p is required for spindle pole body fusion but not for the first mitotic nuclear division during yeast mating (2008)
  • Author(s): S. Nishikawa, A. Hirata, and T. Endo
  • Journal Title: Genes Cells Volume: 13 Pages: 1185-1195
  • DOI: doi:10.1111/j.1365-2443.2008.01236.x
  • Peer Reviewed
• [Journal Article] Arabidopsis thaliana has a set of J proteins in the endoplasmic reticulumthat are conserved from yeast to animals and plants (2008)
  • Author(s): M. Yamamoto, D. Maruyama, T. Endo, and S.Nishikawa
  • Journal Title: Plant Cell Physiol Volume: 49 Pages: 1547-1562
  • DOI: doi:10.1093/pcp/ pcn119
  • Peer Reviewed
• [Journal Article] Step size analyses of the mitochondrial Hsp70 import motor reveal the Brownian ratchet in operation (2008)
  • Author(s): K. Yamano, M. Kuroyanagi-Hasegawa, M. Esaki, M. Yokota, and T. Endo
  • Journal Title: J. Biol. Chem Volume: 283 Pages: 27325-27332
  • DOI: doi:10.1074/jbc. M805249200
  • Peer Reviewed
• [Journal Article] Tom20 and Tom22 share the common signal recognition pathway in mitochondrial protein import (2008)
  • Author(s): K. Yamano, Y. Yatsukawa, M. Esaki, A. E. A Hobbs, R. E. Jensen, and T. Endo
  • Journal Title: J. Biol. Chem Volume: 283 Pages: 3799-3807
  • Peer Reviewed
• [Journal Article] Identification and characterization of a Jemlp ortholog of Candida albicans: dissection of Jemlp functions in karyogamy and protein quality control in Saccharomyces cerevisiae (2008)
  • Author(s): T. Makio, S. Nishikawa, T. Nakayama, H. Nagai, and T. Endo
  • Journal Title: Genes Cells Volume: 13 Pages: 1015-1026
  • Peer Reviewed
• [Journal Article] Tom20 recognizes mitochondrial presequences through dynamic equilibrium among multiple bound states (2007)
  • Author(s): T. Saitoh, M. Igura, T. Obita, T. Ose, R. Kojima, K. Maenaka, T. Endo, and D. Kohda
  • Journal Title: EMBO J Volume: 26 Pages: 4777-4787
  • Peer Reviewed
• [Journal Article] Structural basis of functional cooperation of Tim15/Zim17 with yeast mitochondrial Hsp70 (2007)
  • Author(s): T. Momose, C. Ohshima, M. Maeda, T. Endo
  • Journal Title: EMBO Rep Volume: 8 Pages: 664-670
  • Peer Reviewed
• [Presentation] Organelle homeostasis research in Japan (2013)
  • Author(s): Toshiya Endo
  • Organizer: Organelle Homeostasis Research Center: Workshop on Homeostasis Research
  • Place of Presentation: 福岡
  • Year and Date: 2013-03-23
• [Presentation] New insight into mitochondrial biogenesis Organelle (2013)
  • Author(s): Toshiya Endo
  • Organizer: Homeostasis Research Center: Kick-off Symposium
  • Place of Presentation: 福岡
  • Year and Date: 2013-03-22
• [Presentation] New perspective on import and maintenance of mitochondrial proteins (Invited) (2012)
  • Author(s): Toshiya Endo
  • Organizer: SFB594 3rd International Symposium, Molecular Machines in Protein Folding and Translocation, Munich
  • Place of Presentation: Munich, Germany
  • Year and Date: 20120723-25
• [Presentation] Structural aspects of the dynamic mitochondrial import systems (2012)
  • Author(s): Toshiya Endo
  • Organizer: Gordon Research Conference on Protein transport across cell membranes
  • Place of Presentation: Galveston, US
  • Year and Date: 20120311-16
• [Presentation] Assembly of the TOM40 translocator complex in the mitochondrial outer membrane (Invited) (2012)
  • Author(s): Toshiya Endo
  • Organizer: JST-Vinnova Joint Workshop at BiWO2011
  • Place of Presentation: Tokyo (CBRC)
  • Year and Date: 2012-01-25
• [Presentation] Structural insight into mitochondrial protein traffic in the cell (invited lecture) (2011)
  • Author(s): Toshiya Endo
  • Organizer: Global COE International Symposium on Elucidation and Design of Materials
  • Place of Presentation: 名古屋
  • Year and Date: 20111128-30
• [Presentation] Structural insight into the dynamic mitochondrial protein import pathways (Invited lecture) (2011)
  • Author(s): Toshiya Endo
  • Organizer: EMBO Conference on protein transport systems structures, mechanisms, and medical aspects
  • Place of Presentation: Santa Margherita di Pula, Sardegna, Italy
  • Year and Date: 20110416-20
• [Presentation] Structural insight into mitochondrial protein import (2010)
  • Author(s): Toshiya Endo
  • Organizer: GCOE/Structural Biology Research Center International Symposium: Protein structure and dynamics; from molecules to assembly
  • Place of Presentation: 名古屋
  • Year and Date: 20101123-24
• [Presentation] Structural aspects of import and assembly of mitochondrial proteins (2010)
  • Author(s): Toshiya Endo
  • Organizer: The 3rd International Symposium on Protein Community
  • Place of Presentation: 奈良
  • Year and Date: 20100913-16
• [Presentation] Import and assembly of mitochondrial proteins (invited) (2010)
  • Author(s): Toshiya Endo
  • Organizer: International Symposium Fifty Years of Biophysics Research ad Nagoya University
  • Place of Presentation: 名古屋
  • Year and Date: 20100312-14
• [Presentation] Molecular mechanisms of biogenesis of mitochondria (2010)
  • Author(s): Toshiya Endo
  • Organizer: The 65th West Lake International Symposium of Zhejiang University (The Zhejiang University and Nagoya University Joint Symposium on Life Sciences)
  • Place of Presentation: Hangzhou, China
  • Year and Date: 2010-11-20
• [Presentation] Cooperation of translocators in mitochondrial protein traffic (2009)
  • Author(s): Toshiya Endo
  • Organizer: The 4th International Congress on Stress Responses in Biology and Medicine
  • Place of Presentation: 札幌
  • Year and Date: 20091006-09
• [Presentation] Multiple and sophisticated pathways of mitochondrial protein traffic (2009)
  • Author(s): Toshiya Endo
  • Organizer: Heidelberg Molecular Life sciences Conferences on Cellular Protein Transport
  • Place of Presentation: Heidelberg, Germany
  • Year and Date: 20091001-03
• [Presentation] Role of a chaperone Hsp70 in mitochondrial protein translocation and a folding enzyme PDI in the ER-associated degradation (2009)
  • Author(s): Toshiya Endo
  • Organizer: 2009 International Symposium and Annual Meeting of The Korean Society for Microbiology and Biotechnology
  • Place of Presentation: Daejeon, Korea
  • Year and Date: 20090625-26
• [Presentation] From life of proteins to protein community (Invited lecture) (2009)
  • Author(s): Toshiya Endo
  • Organizer: International Conference "Protein Folding and Neurodegenerative Disease"
  • Place of Presentation: 京都
  • Year and Date: 20090406-07
• [Presentation] Structural basis of molecular recognition in mitochondrial protein import (2009)
  • Author(s): Toshiya Endo
  • Organizer: International Symposium on Molecular Soft Interactions in Biological Systems
  • Place of Presentation: Osaka
  • Year and Date: 20090122-23
• [Presentation] New and old look of mitochondrial protein import (2009)
  • Author(s): Toshiya Endo
  • Organizer: International Symposium Mitochondria and Friends
  • Place of Presentation: Munich
  • Year and Date: 2009-10-30
• [Presentation] Coordinated functions of mitochondrial translocator proteins for protein translocation across and into the membranes (2008)
  • Author(s): Toshiya Endo
  • Organizer: EMBO Conference on Control, Co-ordination and Regulation of Protein Targeting and Translocation
  • Place of Presentation: Saint Maxime, France
  • Year and Date: 20081025-25
• [Presentation] Control and alteration of protein traffic in the cell (2008)
  • Author(s): Toshiya Endo
  • Organizer: The Uehara Memorial Foundaton Symposium "System Biology: The Challenge of Complexity"
  • Place of Presentation: Tokyo
  • Year and Date: 20080630-0702
• [Presentation] How protein import motors function in mitochondrial protein import (2007)
  • Author(s): Toshiya Endo
  • Organizer: FASEB Summer Research Conference : Assembly of the Mitochondrial Respiratory Chain
  • Place of Presentation: Tucson, Arizona, USA
  • Year and Date: 20070805-10
• [Presentation] Pathways and machineries for mitochondrial protein import in yeast, XXII International Conference on Yeast Genetics and Molecular Biology (2007)
  • Author(s): Toshiya Endo
  • Organizer: Symposium Compartmentation of Cellular Activities
  • Place of Presentation: Melbourne
  • Year and Date: 20070701-06
• [Presentation] Functional network of mitochondrial translocator complexes (2007)
  • Author(s): Toshiya Endo
  • Organizer: Gordon Research Conference: Protein Transport Across Cell Membranes
  • Place of Presentation: Il Ciosso
  • Year and Date: 20070610-15
• [Presentation] Complex pathways and machineries for mitochondrial protein import (2007)
  • Author(s): Toshiya Endo
  • Organizer: International Symposium Molecular Machines in Protein Folding and Translocation
  • Place of Presentation: Munich
  • Year and Date: 20070523-25
• [Remarks]
  • URL: http://biochem.chem.nagoya-u.ac.jp/index.html