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2011 Fiscal Year Final Research Report

Quality control of E. coli membrane proteins

Planned Research

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Project AreaProtein community: organization and maintenance of protein functions
Project/Area Number 19058007
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionKyoto University

Principal Investigator

AKIYAMA Yoshinori  京都大学, ウイルス研究所, 教授 (10192460)

Co-Investigator(Kenkyū-buntansha) MORI Hiroyuki  京都大学, ウイルス研究所, 准教授 (10243271)
Project Period (FY) 2007 – 2011
Keywords膜タンパク質 / 品質管理 / 膜プロテアーゼ / ストレス応答 / タンパク質膜透過
Research Abstract

We studied the structures and functions of cellular factors participating in quality control of E. coli membrane proteins. We revealed the cellular functions and substrate recognition mechanisms of intramembrane cleaving proteases, RseP (S2P family) and GlpG (Rhomboid family). We also elucidated the structures of SecYE (translocon) and SecDF, membrane complexes involved in protein translocation, and proposed the mechanism of their actions. In addition, we identified proteins whose functions would be related to ‘membrane stress responses'.

  • Research Products

    (34 results)

All 2011 2010 2009 2008 2007 Other

All Journal Article (14 results) (of which Peer Reviewed: 13 results) Presentation (19 results) Remarks (1 results)

  • [Journal Article] Nascentome analysis uncovers futile protein synthesis in Escherichia coli2011

    • Author(s)
      Ito, K., Chadani, Y., Nakamori, K., Chiba, S., Akiyama, Y., and Abo, T.
    • Journal Title

      PLoS ONE

      Volume: 6 Pages: e28413

    • Peer Reviewed
  • [Journal Article] Post liberation cleavage of signal peptides is catalyzed by the S2P protease in bacteria2011

    • Author(s)
      Saito, A., Hizukuri, Y., Matsuo, E.-i., Chiba, S., Mori, H., Nishimura, O., Ito, K., and Akiyama, Y.
    • Journal Title

      Proc. Natl. Acad. Sci, USA

      Volume: 108 Pages: 13740-13745

    • Peer Reviewed
  • [Journal Article] Structure and function of SecDF, a protein export-enhancing membrane component2011

    • Author(s)
      Tsukazaki, T., Mori, H. , Echizen, Y., Ishitani, R., Fukai, S., Tanaka, T, Perederina, A., Vassylyev, D. G., Kohno, T., Maturana, A. D., Ito, K. and Nureki, O.
    • Journal Title

      Nature

      Volume: 474 Pages: 235-238

    • Peer Reviewed
  • [Journal Article] Recruitment of a species-specific arrest module to monitor different cellular processes2011

    • Author(s)
      Chiba. S., Kanamori, T., Ueda, T., Akiyama, Y., Pogliano, K. and Ito, K.
    • Journal Title

      Proc. Natl. Acad. Sci, USA

      Volume: 108 Pages: 6073-6078

    • Peer Reviewed
  • [Journal Article] Quality control of cytoplasmic membrane proteins in Escherichia coli.2009

    • Author(s)
      Akiyama, Y.
    • Journal Title

      J. Biochem.

      Volume: 46 Pages: 449-454

    • Peer Reviewed
  • [Journal Article] The Sec protein secretion system2009

    • Author(s)
      Ito, K. and Mori, H.
    • Journal Title

      Bacterial Secreted Proteins, Ed. K. Wooldridge

      Pages: 3-22

  • [Journal Article] Substrate recognition and binding by RseP, an Escherichia coli intramembrane protease2008

    • Author(s)
      Koide, K., Ito. K., and Akiyama, Y.
    • Journal Title

      J. Biol. Chem.

      Volume: 283 Pages: 9562-9570

    • Peer Reviewed
  • [Journal Article] Conformational transition of Sec machinery inferred from bacterial SecYE structures2008

    • Author(s)
      Tsukazaki, T., Mori, H., Fukai, S., Ishitani, R., Mori, T., Dohmae, N., Perederina, A., Sugita, Y., Vassylyev, D. G., Ito, K. and Nureki, O.
    • Journal Title

      Nature

      Volume: 455 Pages: 988-991

    • Peer Reviewed
  • [Journal Article] A pair of circularly permutated PDZ domains control RseP, the S2P family intramembrane protease of E. coli.2008

    • Author(s)
      Inaba, K., Suzuki, M., Maegawa, K., Akiyama, S., Ito, K., and Akiyama, Y.
    • Journal Title

      J. Biol. Chem.

      Volume: 283 Pages: 35042-35052

    • Peer Reviewed
  • [Journal Article] The role of L1 loop in the mechanism of rhomboid intramembrane protease GlpG.2007

    • Author(s)
      Wang, Y., Maegawa, S., Akiyama, Y. and Ha, Y.
    • Journal Title

      J. Mol. Biol.

      Volume: 374 Pages: 1104-1113

    • Peer Reviewed
  • [Journal Article] Sequence features of substrates required for cleavage by GlpG, an Escherichia coli rhomboid protease.2007

    • Author(s)
      Akiyama, Y. and Maegawa, S.
    • Journal Title

      Mol. Microbiol.

      Volume: 64 Pages: 1028-1037

    • Peer Reviewed
  • [Journal Article] The intramembrane active site of GlpG, an Escherichia coli rhomboid protease, is accessible to water and hydrolyzes an extramembrane peptide-bond of substrates2007

    • Author(s)
      Maegawa, S., Koide, K., Ito, K., and Akiyama, Y.
    • Journal Title

      Mol. Microbiol.

      Volume: 64 Pages: 435-447

    • Peer Reviewed
  • [Journal Article] Environments of the active site region of RseP, an Escherichia coli RIP protease, assessed by site-directed cysteine alkylation2007

    • Author(s)
      Koide, K., Maegawa, S., Ito, K., and Akiyama, Y.
    • Journal Title

      J. Biol. Chem.

      Volume: 282 Pages: 4553-4560

    • Peer Reviewed
  • [Journal Article] SecY alterations that impair membrane protein folding and generate a membrane stress2007

    • Author(s)
      Shimohata, N., Nagamori, S., Akiyama, Y., Kaback, H.R., and Ito, K.
    • Journal Title

      J. Cell Biol.

      Volume: 176 Pages: 307-317

    • Peer Reviewed
  • [Presentation] Post-liberation cleavage of signal peptides is catalyzed by the site-2 protease (S2P) in bacteria2011

    • Author(s)
      Akiyama, Y.
    • Organizer
      The 18th East Asia Joint Symposium on Biomedical Research
    • Place of Presentation
      Shanghai, China
    • Year and Date
      20111207-09
  • [Presentation] 細菌の蛋白質膜透過装置の構造・機能・病原性との関わり2011

    • Author(s)
      森博幸
    • Organizer
      第48回細菌学会中部支部会
    • Place of Presentation
      名古屋
    • Year and Date
      20111021-22
  • [Presentation] 蛋白質膜透過を促進する膜内在性因子SecDFの構造と機能2011

    • Author(s)
      森博幸、塚崎智也、越前友香、秋山芳展、濡木理、伊藤維昭
    • Organizer
      第84回日本生化学会大会シンポジウム「生体膜エネルギー変換装置の超分子科学」
    • Place of Presentation
      京都
    • Year and Date
      20110921-24
  • [Presentation] Function and regulation of RseP, the S2P family intramembrane protease of E. coli2011

    • Author(s)
      Akiyama, Y. and Hizukuri, Y.
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      20110905-10
  • [Presentation] プロトン駆動力を用いたSecDFによる蛋白質膜透過の昂進機構2011

    • Author(s)
      森博幸、塚崎智也、越前友香、濡木理、伊藤維昭
    • Organizer
      第11回日本蛋白質科学会年会ワークショップ「細胞膜ダイナミクス : In vitro再構成系によるアプローチ」
    • Place of Presentation
      大阪
    • Year and Date
      20110607-09
  • [Presentation] バクテリアの蛋白質膜透過チャネルSecYEGと相互作用する因子の解析2011

    • Author(s)
      森博幸
    • Organizer
      理研シンポジウム「第3回生体分子の分離・解析法の進展-膜タンパク質への応用-」
    • Place of Presentation
      和光
    • Year and Date
      2011-10-28
  • [Presentation] Structure, function and evolution of bacterial protein translocation machinery2010

    • Author(s)
      Mori, H., Tsukazaki, T., Akiyama, Y., Nureki, O. and Ito, K.
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会ワークショップ「進化からみたタンパク質社会」
    • Place of Presentation
      神戸
    • Year and Date
      20101207-10
  • [Presentation] Fate of signal peptides in bacteria: post liberation cleavage by S2P protease.2010

    • Author(s)
      斎藤啓、千葉志信、松尾英一、西村紀、伊藤維昭、秋山芳展
    • Organizer
      第33回日本分子生物学会大会・第83会日本生化学会大会合同大会ワークショップ「膜タンパク質の構造・機能から見るオルガネラの進化」
    • Place of Presentation
      神戸
    • Year and Date
      20101207-10
  • [Presentation] Roles of the two PDZ domains of RseP, the S2P family intramembrane protease of Escherichia coli, in regulation of its protease function2010

    • Author(s)
      Hizukuri, Y. and Akiyama, Y.
    • Organizer
      The 3^<rd> International Symposium on Protein Community
    • Place of Presentation
      Nara, Japan
    • Year and Date
      20100913-16
  • [Presentation] A functionally important intramolecular interaction in SecA : involvement of hydrophobic amino acids in motif IV and anti-parallel beta sheet in translocase activation2010

    • Author(s)
      Mori, H., Tsukazaki, T., Nureki, O., Ito, K. and Akiyama, Y.
    • Organizer
      The 3^<rd> International Symposium on Protein Community
    • Place of Presentation
      Nara
    • Year and Date
      20100913-16
  • [Presentation] 細菌のタンパク質膜透過装置の構造と機能2010

    • Author(s)
      森博幸
    • Organizer
      第4階細菌学若手コロッセウム
    • Place of Presentation
      修善寺
    • Year and Date
      20100826-28
  • [Presentation] New function of RseP, the E. coli S2P family protease2010

    • Author(s)
      Akiyama, Y.
    • Organizer
      Gordon Research Conference on Proteolytic enzymes & their inhibitors
    • Place of Presentation
      Lucca, Italy
    • Year and Date
      20100502-07
  • [Presentation] Molecular mechanisms of SecA mediated protein translocation2009

    • Author(s)
      森博幸、塚崎智也、濡木理、伊藤維昭
    • Organizer
      第47回日本生物物理学会年会シンポジウム「タンパク質の膜透過輸送の最前線」
    • Place of Presentation
      徳島
    • Year and Date
      20091030-1101
  • [Presentation] バクテリアのタンパク質膜透過装置の構造と機能2009

    • Author(s)
      森博幸、塚崎智也、越前友香、濡木理、伊藤維昭
    • Organizer
      第82回日本生化学会大会シンポジウム「タンパク質膜透過装置の構造とダイナミックな機能」
    • Place of Presentation
      神戸
    • Year and Date
      20091021-24
  • [Presentation] Functional dissection of bacterial Sec translocation machinery2009

    • Author(s)
      Mori H., Tsukazaki T., Echizen, Y., Ishitani, R., Nureki, O. and Ito, K.
    • Organizer
      Innovative Nanoscience of Supermolecular Motor Proteins Working in Biomembranes
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      20090908-10
  • [Presentation] 大腸菌RIPプ「直接性、不可逆性を特徴とする新しいシグナル伝達のメカニズム」2008

    • Author(s)
      秋山芳展
    • Organizer
      第31回日本神経科学大会
    • Place of Presentation
      東京
    • Year and Date
      20080709-11
  • [Presentation] How does SecA ATPase interact with translocon and drive protein translocation?2008

    • Author(s)
      Mori, H.
    • Organizer
      G-COE in Chemistry "Frontier of Organelle Dynamics and Protein Functions"
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      20080311-13
  • [Presentation] Function and regulation of E. coli membrane metalloproteases2007

    • Author(s)
      Akiyama, Y.
    • Organizer
      Gordon Research Conference on Matrix metalloproteinases
    • Place of Presentation
      Lucca, Italy
    • Year and Date
      20070603-08
  • [Presentation] How does SecA ATPase drive protein translocation?2007

    • Author(s)
      Mori, H. and Ito, K.
    • Organizer
      The 14th East Asia Joint Symposium on Biomedical Research
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2007-11-14
  • [Remarks]

    • URL

      http://www.virus.kyoto-u.ac.jp/Lab/akiyama/index.html

URL: 

Published: 2013-07-31  

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