2011 Fiscal Year Final Research Report
Quality control mechanism of misfolded proteins in the ER
| Project Area | Protein community: organization and maintenance of protein functions |
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| Project/Area Number |
19058008
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Kyoto Sangyo University (2011)
Kyoto University (2007-2010) |
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Principal Investigator |
NAGATA Kazuhiro 京都産業大学, 総合生命科学部, 教授 (50127114)
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| Co-Investigator(Kenkyū-buntansha) |
INABA Kenji 九州大学, 生体防御医学研究所, 特任准教授 (10423039)
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| Project Period (FY) |
2007 – 2011
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| Keywords | 小胞体 / 品質管理 / 小胞体関連分解 / レドックス制御 / 分子シャペロン |
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| Research Abstract |
We analyzed the molecular mechanism of ER-associated degradation (ERAD). In addition to EDEM, which recruits the misfolded proteins from productive folding pathway to degradation pathway, we also found a novel reductase in the ER, ERdj5, is also involved in the acceleration of ERAD by cleaving disulfide bonds of the substrates. By using biochemical and structural analysis, we have revealed the substrate transfer pathway in the ERAD system; misfolded proteins are transferred from calnexin to EDEM1, ERdj5, BiP and finally to SEL1L in the ERAD complex.
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