Analyses of physiological roles of unfolded protein response.

2009 Fiscal Year Self-evaluation Report

• Project Area: Protein community: organization and maintenance of protein functions
• Project/Area Number: 19058010
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: KOHNO Kenji Nara Institute of Science and Technology, バイオサイエンス研究科, 教授 (50142005)
• Project Period (FY): 2007 – 2012
• Keywords: 小胞体ストレス / 蛋白質品質管理 / シグナル伝達 / 転写翻訳調節 / HSP40

Research Abstract

(1)小胞体内の異常タンパク質感知機構や動物組織における各センサーのUPR(Unfolded Protein Response:異常タンパク質応答)の解析、(2)発生・分化や各種組織の機能維持におけるUPR経路活性化の生理的役割の解明、(3)UPR時における小胞体やサイトゾルHsp40の生理機能、の3点に研究を集約して進める。具体的には、小胞体センサーIRE1は異常タンパク質の何を認識しているのか、IRE1の活性化機構、XBP1 mRNAのサイトゾルにおけるスプライシング機構、UPR からアポトシスに至る機構の解析、肝臓や膵臓におけるUPR の生理的意味や役割、Hsp40の動物個体における生理機能を明らかにすることを目的とする。

Research Products

• [Journal Article] Function of IRE1alpha in the placenta is essential for placental development and embryonic viability. (2009)
  • Author(s): Iwawaki, T., Akai, R., Yamanaka, S., Kohno, K.
  • Journal Title: Proc Natl Acad Sci USA 106 Pages: 16657-16662
  • Peer Reviewed
• [Journal Article] Cotranslational targeting of XBP1 protein to the membrane promotes cytoplasmic splicing of its own mRNA. (2009)
  • Author(s): Yanagitani, K., Imagawa, Y., Iwawaki, T., Hosoda, A., Saito, M., Kimata, Y., Kohno, K.
  • Journal Title: Mol. Cell 34 Pages: 191-200
  • Peer Reviewed
• [Journal Article] Two regulatory steps of ER-stress sensor Ire1 involving its cluster formation and binding to unfolded proteins. (2007)
  • Author(s): Kimata, Y., Ito, T., Ishiwata-Kimata, Y., Suzuki, T., Oikawa, D., Takeuchi, M., Kohno, K.
  • Journal Title: J. Cell Biol. 179 Pages: 75-86
  • Peer Reviewed
• [Presentation] IRE1βis a key molecule for ER quality control in goblet cell. (2009)
  • Author(s): Kenji Kohno
  • Organizer: The 4th International Congress on Stress Responses in Biology and Medicine.
  • Place of Presentation: Sapporo(invited)
  • Year and Date: 2009-10-07
• [Presentation] Dynamic regulation of XBP1 mRNA distribution in the ER stress response. (2009)
  • Author(s): Kenji Kohno
  • Organizer: Gordon Research Conferences: Stress Proteins in Growth, Development & Desease
  • Place of Presentation: Proctor Academy, NH, USA(invited)
  • Year and Date: 2009-07-01
• [Book] 蛋白質核酸酵素 (2009)
  • Author(s): 柳谷耕太、河野憲二
  • Total Pages: 7
  • Publisher: 共立出版
• [Remarks]
  • URL: http://bsw3.naist.jp/kouno/kouno.html
• [Patent(Industrial Property Rights)] Diabetes model animal. United States Patent (2010)
  • Inventor(s): 河野憲二
  • Industrial Property Rights Holder: 奈良先端科学技術大学院大学
  • Industrial Property Number: Patent・No: US 7, 642, 401 B2
  • Acquisition Date: 2010-01-05