Peroxisome biogenesis and regulation

2011 Fiscal Year Final Research Report

• Project Area: Protein community: organization and maintenance of protein functions
• Project/Area Number: 19058011
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyushu University
• Principal Investigator: FUJIKI Yukio 九州大学, 大学院・理学研究院, 教授 (70261237)
• Co-Investigator(Kenkyū-buntansha): TAMURA Shigehiko 九州大学, 大学院・理学研究院, 准教授 (90236753) ; OKUMOTO Kanji 九州大学, 大学院・理学研究院, 助教 (20363319)
• Project Period (FY): 2007 – 2011
• Keywords: ペルオキシソームの形成機構 / CHO 変異細胞 / ペルオキシン / Pex5p リサイクル / ユビキチン化 / ペルオキシソームの分裂と形態制御 / DHA / 品質管理

Research Abstract

Our representative findings include 1) peroxisomal membrane is assembled by Pex3p, Pex16p and Pex19p; 2) in peroxisome-cytoplasmic shuttling of matrix protein transporter Pex5p, Cys-ubiquitination of Pex5p and soluble factor AWP1 are essential for the Pex5p exit.

Research Products

• [Journal Article] Ligand-dependent nucleo-cytoplasmic shuttling of peroxisome proliferators-activated receptors, PPARα and PPARγ (2012)
  • Author(s): Umemoto, T., and Fujiki, Y.
  • Journal Title: Genes Cells Volume: (in press)
  • Peer Reviewed
• [Journal Article] Peroxisome biogenesis disorders: molecular basis for impaired peroxisomal membrane assembly (2012)
  • Author(s): Fujiki, Y., Yagita, Y., and Matsuzaki, T.
  • Journal Title: Biochim. Biophys. Acta- Mol. Basis Dis. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Defective lipid remodeling of GPI anchors in peroxisomal disorders, Zellweger syndrome and rhizomelic chondrodysplasia punctata (2012)
  • Author(s): Kanzawa, N., Shimozawa, N., Wanders, R.J.A., Ikeda, K., Murakami, Y., Waterham, H.R., Mukai, S., Fujita, M., Maeda, Y., Taguchi, R., Fujiki, Y., and Kinoshita, T.
  • Journal Title: J. Lipid Res. Volume: 53 Pages: 653-663
  • Peer Reviewed
• [Journal Article] Docosahexaenoic acid mediates peroxisomal elongation, a prerequisite for peroxisome division (2012)
  • Author(s): Itoyama, A., Honsho, M., Abe, Y., Moser, A., Yoshida, Y., and Fujiki, Y.
  • Journal Title: J. Cell Sci. Volume: 125 Pages: 589-602
  • Peer Reviewed
• [Journal Article] AWP1/ZFAND6 functions in Pex5 export by interacting with Cys-monoubiquitinated Pex5 and Pex6 AAA ATPase (2012)
  • Author(s): Miyata, N., Okumoto, K., Noguchi, M., Mukai, S., and Fujiki, Y.
  • Journal Title: Traffic Volume: 13 Pages: 168-183
  • Peer Reviewed
• [Journal Article] New insights into dynamic and functional assembly of the AAA peroxins, Pex1p and Pex6p, and their membrane receptor Pex26p in shuttling of PTS1-receptor Pex5p during peroxisome biogenesis (2012)
  • Author(s): Fujiki, Y., Nashiro, C., Miyata, N., Tamura, S., Okumoto, K.
  • Journal Title: Biochim. Biophys. Acta- Mol. Cell Biol. Volume: 1823 Pages: 145-149
  • Peer Reviewed
• [Journal Article] Two proteases, Tysnd1 and PsLon, cooperatively regulate fatty-acid β-oxidation in the peroxisomal matrix (2011)
  • Author(s): Okumoto, K., Kametani, Y., and Fujiki, Y.
  • Journal Title: J. Biol. Chem. Volume: 286 Pages: 44367-44379
  • Peer Reviewed
• [Journal Article] ペルオキシソームの形成・制御の分子基盤 (2011)
  • Author(s): 藤木幸夫, 糸山彰徳, 奥本寛治
  • Journal Title: 細胞工学11 月号特集「オルガネラモデリング- ベールを脱ぐ分子設計」 Volume: 30 Pages: 1153-1159
  • Peer Reviewed
• [Journal Article] Sec16B is involved in the endoplasmic reticulum export of the peroxisomal membrane biogenesis factor peroxin 16 (Pex16) in mammalian cells (2011)
  • Author(s): Yonekawa, S., Furuno, A., Baba, T., Fujiki, Y., Ogasawara, Y., Yamamoto, A., Tagaya, M., and Tani, K.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 108 Pages: 12746-12751
  • Peer Reviewed
• [Journal Article] Cysteine- ubiquitination of peroxisome- targeting-signal type1 (PTS1)- receptor Pex5p regulates Pex5p recycling. (2011)
  • Author(s): Okumoto, K., Misono, S., Miyata, N., Matsumoto, Y., Mukai, S., and Fujiki, Y.
  • Journal Title: Traffic Volume: 12 Pages: 1067-1083
  • Peer Reviewed
• [Journal Article] Recruiting mechanism of the AAA peroxins, Pex1p and Pex6p, to Pex26p on peroxisome membrane. (2011)
  • Author(s): Nashiro, C., Kashiwagi, A., Matsuzaki, T., Tamura, S., and Fujiki, Y.
  • Journal Title: Traffic Volume: 12 Pages: 774-788
  • Peer Reviewed
• [Journal Article] Nuclear transport of peroxisome- proliferator activated receptor α. (2011)
  • Author(s): Iwamoto, F., Umemoto, T., Motojima, K., and Fujiki, Y.
  • Journal Title: J. Biochem. Volume: 149 Pages: 311-319
  • Peer Reviewed
• [Journal Article] Interaction defect of the medium isoform of PTS1-receptor Pex5p with PTS2- receptor Pex7p abrogates the PTS2 protein import into peroxisomes in mammals. (2011)
  • Author(s): Honsho, M., Hashiguchi, Y., Ghaedi, K., and Fujiki Y.
  • Journal Title: J. Biochem. Volume: 149 Pages: 203-210
  • Peer Reviewed
• [Journal Article] ペルオキシソームの形成・制御とその障害による高次機能の破綻 (2010)
  • Author(s): 藤木幸夫, 宮田暖, 松園裕嗣, 松崎高志, 本庄雅則
  • Journal Title: 実験医学8 月号特集「Protein kinesis を解き明かすオルガネラの世界- 細胞機能の制御と遺伝病発症・ウイルス感染のメカニズム」 Volume: 28 Pages: 2094-2101
  • Peer Reviewed
• [Journal Article] A novel fluorescent sensor protein for visualization of redox states in the cytoplasm and in peroxisomes. (2010)
  • Author(s): Yano, T., Oku, M., Akeyama, N., Itoyama, A., Yurimoto, H., Kuge, S., Fujiki, Y., and Sakai, Y.
  • Journal Title: Mol. Cell. Biol. Volume: 30 Pages: 3758-3766
  • Peer Reviewed
• [Journal Article] Monomerdimer transition of the consered N-terminal domain of the mammalian peroxisomal matrix protein import receptor, Pex14p. (2010)
  • Author(s): Su, J. R., Takeda, K., Tamura, S., Fujiki, Y., and Miki, K.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 394 Pages: 217-221
  • Peer Reviewed
• [Journal Article] Posttranslational regulation of fatty Acyl-CoA reductase 1, Far1, controls ether glycerophospholipid synthesis. (2010)
  • Author(s): Honsho, M., Asaoku, S., and Fujiki, Y.
  • Journal Title: J. Biol. Chem. Volume: 285 Pages: 8537-8542
  • Peer Reviewed
• [Journal Article] In vitro import of peroxisome-targeting signal type 2 (PTS2) receptor Pex7p into peroxisomes. (2009)
  • Author(s): Miyata, N., Hosoi, K., Mukai, S., and Fujiki, Y.
  • Journal Title: Biochim. Biophys. Acta- Mol. Cell Res. Volume: 1793 Pages: 860-870
  • Peer Reviewed
• [Journal Article] Crystal struture of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. (2009)
  • Author(s): Su, J. R., Takeda, K., Tamura, S., Fujiki, Y., and Miki, K.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 106 Pages: 417-421
  • Peer Reviewed
• [Journal Article] The peroxisomal membrane-protein import receptor Pex3p is directly transported to peroxisomes by a novel Pex19p- and Pex16p-dependent pathway. (2008)
  • Author(s): Matsuzaki, T., and Fujiki, Y.
  • Journal Title: J. Cell Biol. Volume: 183 Pages: 1275-1286
  • Peer Reviewed
• [Journal Article] Rcruitment of the RNA helicase RHAU to stress granules via a unique RNA-binding domain. (2008)
  • Author(s): Chalupnikova, K., Lattmann, S., Selak, N., Iwamoto, F., Fujiki, Y., and Nagamine, Y.
  • Journal Title: J. Biol. Chem. Volume: 283 Pages: 35186-35198
  • DOI: .
  • Peer Reviewed
• [Journal Article] The peroxin Pex14p is involved in LC3-dependent degradation of mammalian peroxisomes. (2008)
  • Author(s): Hara-Kuge, S., and Fujiki, Y.
  • Journal Title: Exp. Cell Res. Volume: 314 Pages: 3531-3541
  • Peer Reviewed
• [Journal Article] Isolation and characterization of mutant animal cell line defective in alkyl-dihydroxyacetonephosphate synthase: Localization and transport of plasmalogens. (2008)
  • Author(s): Honsho, M., Yagita, Y., Kinoshita, N., and Fujiki, Y.
  • Journal Title: Biochim. Biophys. Acta- Mol. Cell Res. Volume: 1783 Pages: 1857-1865
  • Peer Reviewed
• [Journal Article] Isolation and characterization of novel phenotype CHO cell mutants defective in peroxisome assembly, using ICR191 as a potent mutagenic agent. (2008)
  • Author(s): Ghaedi, K., and Fujiki. Y.
  • Journal Title: Cell Biochem. Funct. Volume: 26 Pages: 684-691
  • Peer Reviewed
• [Journal Article] Characterization of the interaction between recombinant human peroxin PEX3p and PEX19p: Identification of TRP104 in Pex3p as a critical residue for the interaction. (2008)
  • Author(s): Sato, Y., Shibata, H., Nakano, H., Matsuzono, Y., Yoshinori, K., Kobayashi, Y., Fujiki, Y., Imanaka, T., and Kato, H.
  • Journal Title: J. Biol. Chem. Volume: 283 Pages: 6136-6144
  • Peer Reviewed
• [Journal Article] Dynamic and functional assembly of the AAA peroxins, Pex1p and Pex6p, and their membrane receptor Pex26p involved in shuttling of the PTS1 receptor Pex5p in peroxisome biogenesis. (2008)
  • Author(s): Fujiki, Y., Miyata, N., Matsumoto, N., and Tamura, S.
  • Journal Title: Biochem. Soc. Trans. Volume: 36 Pages: 109-113
  • Peer Reviewed
• [Journal Article] Alterations in the molecular species of plasmalogen phospholipids and glycolipids due to peroxisomal dysfunction in Chinese hamster ovary-mutant Z65 cells by FABMS method. (2007)
  • Author(s): Saito, M., Horikawa, M., Iwamori, Y., Sakakihara, Y., Mizuguchi, M., Igarashi, T., Fujiki, Y., and Iwamori, M.
  • Journal Title: J. Chromatogr. B Volume: 852 Pages: 367-373
  • Peer Reviewed
• [Journal Article] Fis1, DLP1, and Pex11p coordinately regulate peroxisome morphogenesis. (2007)
  • Author(s): Kobayashi, S., Tanaka, A., and Fujiki, Y.
  • Journal Title: Exp. Cell Res. Volume: 313 Pages: 1675-1686
  • Peer Reviewed
• [Presentation] Peroxisome: Biogenesis, Homeostasis, and Peroxisome Biogenesis Disorders (2012)
  • Author(s): Yukio Fujiki
  • Organizer: The 7th KOREA-JAPAN Conference on Cellular Signaling for Young Scientists
  • Place of Presentation: 韓国ウルサン市
  • Year and Date: 20120216-18
• [Presentation] ペルオキシソームおホメオスタシス：マトリクス酵素の調節とオルガネラ分解 (2011)
  • Author(s): 藤木幸夫, 山下俊一, 久下小百合, 奥本寛治
  • Organizer: 第34 回日本分子生物学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 20111213-16
• [Presentation] AAA peroxins, Pex1p and Pex6p, and Their Recruiter Pex26p Modulate the Peroxisomal Targeting Signal 1 (PTS1) Receptor Pex5p in Peroxisomal Protein Import. (2011)
  • Author(s): Yukio Fujiki
  • Organizer: The 9th International Conference on AAA Proteins
  • Place of Presentation: 熊本市
  • Year and Date: 20111106-10
• [Presentation] ペルオキシソームのホメオスタシス：タンパク質インポートおよび形態の調節 (2011)
  • Author(s): 藤木幸夫, 宮田暖, 糸山彰徳, 奥本寛治
  • Organizer: 第84 回日本生化学会大会
  • Place of Presentation: 京都市
  • Year and Date: 20110921-24
• [Presentation] Peroxisome biogenesis: membrane assembly and matrix protein import - Lessons from different species (2010)
  • Author(s): 藤木幸夫
  • Organizer: 第33 回日本分子生物学会年会・第83 回日本生化学会合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 20101207-10
• [Presentation] Peroxisome: biogenesis and homeostasis - membrane assembly, matrix protein import, morphogenesis, and turnover (2010)
  • Author(s): Yukio Fujiki
  • Organizer: The 3^<rd> International Symposium on Protein Community
  • Place of Presentation: 奈良市
  • Year and Date: 20100912-16
• [Presentation] 細胞内小器官ペルオキシソームの形成・障害・ホメオスタシス (2010)
  • Author(s): 藤木幸夫
  • Organizer: 平成22 年度日本生化学会中国・四国支部会例会
  • Place of Presentation: 山口市
  • Year and Date: 20100514-15
• [Presentation] Peroxisomal import of matrix and membrane proteins (2010)
  • Author(s): Yukio Fujiki
  • Organizer: Gordon Research Conferences: Protein transport across cell membranes
  • Place of Presentation: 米国ガルベストン市
  • Year and Date: 20100307-12
• [Presentation] ペルオキシソームの創成とその制御機構 (2009)
  • Author(s): 藤木幸夫
  • Organizer: 第32 回日本分子生物学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 20091209-12
• [Presentation] Peroxisome biogenesis and its regulation: membrane assembly, matrix protein import, and morphogenesis (2009)
  • Author(s): Yukio Fujiki
  • Organizer: International Meeting on Peroxisome Research
  • Place of Presentation: 米国シアトル市
  • Year and Date: 20091118-20
• [Presentation] ペルオキシソーム形成におけるPex5p のダイナミズムとその制御システム (2009)
  • Author(s): 田村茂彦
  • Organizer: 第82 回日本生化学会大会
  • Place of Presentation: 神戸市
  • Year and Date: 20091021-24
• [Presentation] Peroxisome biogenesis and Its Dysfunctions: Membrane Assembly, Matrix Protein Import, and Morphogenesis (2009)
  • Author(s): Yukio Fujiki
  • Organizer: The 4^<th> International Congress on Stress Responses in Biology and Medicine
  • Place of Presentation: 札幌市
  • Year and Date: 20091006-09
• [Presentation] Degradation of Mammalian Peroxisomes (2009)
  • Author(s): Yukio Fujiki
  • Organizer: The 5^<th> International Symposium on Autophagy
  • Place of Presentation: 大津市
  • Year and Date: 20090924-28
• [Presentation] Dynamic and functional assembly of the AAA Peroxins, Pex1p and Pex6p, and their interacting partners in peroxisome biogenesis (2009)
  • Author(s): Yukio Fujiki
  • Organizer: 8^<th> International Conference on AAA Proteins
  • Place of Presentation: カナダトロント市
  • Year and Date: 20090712-16
• [Presentation] Peroxisome Biogenesis: Mechanistic insights to protein import and its regulation (2009)
  • Author(s): 藤木幸夫
  • Organizer: 第61 回日本細胞生物学会大会
  • Place of Presentation: 名古屋市
  • Year and Date: 20090602-04
• [Presentation] ペルオキシソームの生合成：膜アセンブリーとマトリックスタンパク質輸送の分子基盤 (2008)
  • Author(s): 藤木幸夫
  • Organizer: 第31 回日本分子生物学会年会・第81 回日本生化学会合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 20081209-12
• [Presentation] Roles of AAA peroxins and Pex26p in peroxisome biogenesis (2008)
  • Author(s): Shigehiko Tamura
  • Organizer: Frontier of Organelle Dynamics and Protein Functions
  • Place of Presentation: 名古屋市
  • Year and Date: 20080311-13
• [Presentation] Peroxisome Biogenesis and Dysfunction: Membrane Assembly, Matrix Protein Import, Morphogenesis, and Peroxisome Biogenesis Disorders (2007)
  • Author(s): 藤木幸夫
  • Organizer: 第30 回日本分子生物学会年会・第80 回日本生化学会合同大会
  • Place of Presentation: 横浜市
  • Year and Date: 20071211-15
• [Presentation] Dynamic and Functional Assembly of the AAA Peroxins, Pex1p and Pex6p, and Their Membrane Receptor Pex26p in Peroxisome Biogenesis (2007)
  • Author(s): Yukio Fujiki, Naomi Matsumoto, Shigehiko Tamura
  • Organizer: The 7^<th> International AAA Proteins Conference
  • Place of Presentation: イギリスサイレンセスター市
  • Year and Date: 20070909-13
• [Presentation] Peroxisome Biogenesis: Matrix Protein Import, Membrane Assembly, Morphogenesis, and Peroxisome Assembly Disorders (2007)
  • Author(s): Yukio Fujiki
  • Organizer: Gordon Research Conferences: Protein transport across cell membranes
  • Place of Presentation: イタリアバルガ市
  • Year and Date: 20070610-15
• [Presentation] Peroxisome Biogenesis: Membrane Assembly, Matrix Protein Import, Morphogenesis, and Peroxisome Assembly Disorders (2007)
  • Author(s): 藤木幸夫
  • Organizer: 第40 回日本発生生物学会・第59 回日本細胞生物学会合同大会
  • Place of Presentation: 福岡市
  • Year and Date: 20070528-30
• [Book] 生物薬科学実験講座５ 細胞の構造とオルガネラ 第２章オルガネラ形成機構の研究法「ペルオキシソーム形成機構の研究法」pp.148-171, 大熊勝治/中西義信 編 (2010)
  • Author(s): 藤木幸夫
  • Publisher: 廣川書店
• [Remarks]
  • URL: http://www.biology.kyushu-u.ac.jp/~taisha/