Reconstitution of Human Immune System in NOG Mice

2009 Fiscal Year Self-evaluation Report

• Project Area: Immunological Self Recognition and its Disorders
• Project/Area Number: 19059001
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: 地方独立行政法人宮城県立病院機構 宮城県立がんセンター(研究所) (2011); Miyagi Cancer Center Research Institute (2009-2010); Tohoku University (2007-2008)
• Principal Investigator: SUGAMURA Kazuo Tohoku University, 免疫学部, 総長 (20117360)
• Project Period (FY): 2007 – 2011
• Keywords: ヒト免疫系 / ヒト化マウス / 免疫不全症

Research Abstract

免疫不全NOGマウスにヒト血液幹細胞を移植し、マウス中でヒト免疫系を再構築する。すなわち免疫系ヒト化マウスの作成を目指す。また、免疫系ヒト化マウスは骨髄中のヒト血液幹細胞の分化を解析するにあたり、理想的なシステムとなることから原発性免疫不全症や、T細胞の分化異常を伴うDiGeorge症候群、好中球の分化異常を伴うCongenital Neutropeniaなど、細胞性免疫・自然免疫系疾患のヒト化病態モデルを作製する。これを基に細胞療法や遺伝子治療法の開発を行う。

Research Products

• [Journal Article] Induction of cell cycle entry eliminates human leukemia stem cells in a mouse model of AML. (2010)
  • Author(s): Saito Y, Ishikawa F, et al
  • Journal Title: Nature Biotechnology 28-3 Pages: 275-280
• [Journal Article] Highly sensitive model for xenogenic GVHD using severe immunodeficient NOG mice. (2009)
  • Author(s): Ito R, Ito M, et al
  • Journal Title: Transplantation 87 Pages: 1654-1658
• [Journal Article] The analysis of the functions of human B and T cells in humanized NOD/shi-scid/gammac(null) (NOG) mice (hu-HSC NOG mice). (2009)
  • Author(s): Watanabe Y, Takahashi T, Ito M, Sugamura K, et al
  • Journal Title: Int. Immunol. 21 Pages: 843-858
• [Journal Article] NOD/Shi-scid IL2rgamma(null) (NOG) mice more appropriate for humanized mouse models. (2008)
  • Author(s): Ito M, Kobayashi K, Nakahata T
  • Journal Title: Curr Top Microbiol Immunol 324 Pages: 53-76
• [Journal Article] Chemotherapy-resistant human AML stem cells home to and engraft within the bone marrow endosteal region. (2007)
  • Author(s): Ishikawa F, et al
  • Journal Title: Nature Biotechnology 25 Pages: 1315-1321
• [Presentation] High sensitivity to xeno-GVHD in severely immunodeficient NOG mice attributable to dysfunction of dendritic cells. (2009)
  • Author(s): Ito R, Ito M, et al
  • Organizer: 第2回ヒト化マウス国際ワークショップ
  • Place of Presentation: オランダ
  • Year and Date: 20090403-20090406
• [Presentation] The analysis of the functions of human B and T cells in humanized NOD/shi/γcnull (NOG) mice (hu-HSC NOG mice). (2009)
  • Author(s): Takahashi T, Ito M, Sugamura K, et al
  • Organizer: 第2回ヒト化マウス国際ワークショップ
  • Place of Presentation: オランダ
  • Year and Date: 20090403-20090406
• [Presentation] Translational medicine using humanized mouse (2009)
  • Author(s): 石川文彦
  • Organizer: 第39回日本免疫学会総会・学術集会シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-03
• [Book] The differentiative and regenerative properties of human hematopoietic stem/progenitor cells in the NOD-SCID/IL2rgnull mice (2008)
  • Author(s): Ishikawa F, et al
  • Total Pages: 87-94
  • Publisher: Springer
• [Patent(Industrial Property Rights)] 白血病幹細胞マーカー (2009)
  • Inventor(s): 石川文彦、小原収、齊藤頼子、北村浩、土方敦司、小澤秀俊
  • Industrial Property Rights Holder: 独立行政法人理化学研究所
  • Industrial Property Number: 特願2009-072400
  • Filing Date: 2009-03-24
• [Patent(Industrial Property Rights)] 白血病の再発予防薬 (2009)
  • Inventor(s): 石川文彦、齊藤頼子、Shultz LD
  • Industrial Property Rights Holder: 独立行政法人理化学研究所、Shultz LD
  • Industrial Property Number: 特願2009-052723
  • Filing Date: 2009-03-05
• [Patent(Industrial Property Rights)] 「ヒト白血病幹細胞および白血病非幹細胞が増幅されたマウスおよびその製造方法」 (2007)
  • Inventor(s): 石川文彦、齊藤頼子(PCT:石川文彦、齊藤頼子、小原収、Shultz LD)
  • Industrial Property Rights Holder: 独立行政法人理化学研究所、Shultz LD
  • Industrial Property Number: 特願2007-271870
  • Filing Date: 2007-10-01