Regulatory system that controls cell proliferation and leaf development in the shoot apical meristem of Arabidopsis thaliana

2009 Fiscal Year Self-evaluation Report

• Project Area: Plant regulatory systems that control developmental interactions between meristems and lateral organs
• Project/Area Number: 19060003
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Nagoya University
• Principal Investigator: MACHIDA Yasunori Nagoya University, 大学院・理学研究科, 教授 (80175596)
• Project Period (FY): 2007 – 2012
• Keywords: 葉の発生分化 / miRNA / 遺伝子発現制御 / 葉の左右相称性 / 葉の表裏 / DNAのメチル化 / 細胞分裂周期

Research Abstract

本研究計画の目的は、茎頂メリステム(幹細胞群)において葉原基が発生し、葉の三次元構造(基部-先端部軸、向軸-背軸、中央-側方軸)が生み出される分子機構を解明することである。これまでに、3つの軸形成には、シロイヌナズナのASYMMETRIC LEAVES1(AS1)とASYMMETRIC LEAVES2(AS2)が重要な役割を担っていることを明らかにしてきた。本研究では、AS1とAS2を基軸として、葉の3つの軸形成の制御機構、細胞分化のエピジェネティックな抑制機構について明らかにする。さらに、細胞分化と分裂のタイミングについて関連性を明らかにする。

Research Products

• [Journal Article] Characterization of genes in the ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES (AS2/LOB) family in Arabidopsis thaliana and functional and molecular comparisons between AS2 and other family members. (2009)
  • Author(s): Matsumura, Y., Iwakawa, H., Machida, Y., Machida, C.
  • Journal Title: Plant J. 58 Pages: 525-537
• [Journal Article] Microtubule-associated kinase-like protein RUNKEL needed for cell plate expansion in Arabidopsis cytokinesis. (2009)
  • Author(s): Krupnova, T., Sasabe, M., Ghebreghiorghis, L., Gruber, C.W., Hamada, T., Dehmel, V., Strompen, G., Stierhof, Y.-D. Lukowitz, W., Kemmerling, B., Machida, Y., Hashimoto, T., Mayer, U., Jurgens, G.
  • Journal Title: Curr. Biol. 19 Pages: 518-523
• [Journal Article] bC1, the pathogenicity factor of TYLCCNV, interacts with AS1 to alter leaf development and suppress selective jasmonic acid responses. (2008)
  • Author(s): Yang, J.-Y., Iwasaki, M., Machida, C., Machida, Y., Zhou, X., Chua, N.-H.
  • Journal Title: Genes Dev. 22 Pages: 2564-2577
• [Journal Article] Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves. (2007)
  • Author(s): Iwakawa, H., Iwasaki, M., Kojima, S., Ueno, Y., Soma, T., Tanaka, H., Semiarti, E., Machida, Y., Machida, C.
  • Journal Title: Plant J. 51 Pages: 173-184
• [Journal Article] Novel receptor-like kinase ALE2 controls shoot development by specifying epidermis in Arabidopsis. (2007)
  • Author(s): Tanaka, H., Watanabe, M., Sasabe, M., Hiroe, T., Tanaka, T., Tsukaya, T., Ikezaki, M., Machida, C., Machida, Y.
  • Journal Title: Development 134 Pages: 1643-1652
• [Journal Article] Histone deacetylases and ASYMMETRIC LEAVES2 are involved in the establishment of polarity in leaves of Arabidopsis. (2007)
  • Author(s): Ueno, Y., Ishikawa, T., Watanabe, K., Terakura, S., Iwakawa, H., Okada, K., Machida, C., Machida, Y.
  • Journal Title: Plant Cell 19(2) Pages: 445-457
• [Presentation] シロイヌナズナasymmetric leaves2変異体の向背軸性異常はrRNA前駆体のプロセシングに関わる遺伝子の変異により亢進される (2010)
  • Author(s): 町田泰則, 他7名
  • Organizer: 第51回日本植物生理学会
  • Place of Presentation: 熊本大学黒髪北キャンパス、熊本市
  • Year and Date: 20100318-20100321
• [Presentation] The sequences in the AS2/LOB domain are required for thelocalization to the AS2 body and the functions of ASYMMETRIC LEAVES2 (AS2) protein (2009)
  • Author(s): Lilan Luo, 他7名
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜、横浜市
  • Year and Date: 20091209-20091212
• [Presentation] シロイヌナズナのENHANCER OF ASYMMETRIC LEAVES1 AND ASYMMETRIC LEAVES2 (EAL)はAS1やAS2とともに葉の向背軸性の確立に関与する (2009)
  • Author(s): 石橋奈々子, 他4名
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜、横浜市
  • Year and Date: 20091209-20091212
• [Presentation] ASYMMETRIC LEAVES2 (AS2) and AS1 of Arabidopsis are Involved in Epigenetic Control of Leaf Development (2009)
  • Author(s): Machida, Y., 他7名
  • Organizer: a symposium on "Epigenetics and RNA Biology in Plants" (joint symposium between Korean Society of Botany and Korean Society of Plant Biotechnology)
  • Place of Presentation: Kwang-ju, Korea
  • Year and Date: 20090430-20090501
• [Book] 「植物ゲノム科学辞典」(編集及び部分執筆) (2009)
  • Author(s): 駒嶺穆、斉藤和季、田畑哲之、藤村達人、町田泰則、三位正洋
  • Publisher: 朝倉書店
• [Remarks]
  • URL: http://www.bio.nagoya-u.ac.jp:/~yas/tokutei_plant_meristems/
• [Patent(Industrial Property Rights)] ラン科植物の形質転換方法 (2008)
  • Inventor(s): 町田泰則、町田千代子、Endang Semiarti
  • Industrial Property Rights Holder: 国立大学法人名古屋大学
  • Industrial Property Number: PCT/JP2008/056227
  • Filing Date: 2008-03-28
• [Patent(Industrial Property Rights)] 形質転換体の作出方法及びその利用 (2007)
  • Inventor(s): 町田泰則、上野宜久
  • Industrial Property Rights Holder: 国立大学法人名古屋大学
  • Industrial Property Number: 特願2007-006588
  • Filing Date: 2007-01-16