情報統御分子の伝搬器官としての維管束系の分化

2010 Fiscal Year Annual Research Report

• Project Area: Plant regulatory systems that control developmental interactions between meristems and lateral organs
• Project/Area Number: 19060009
• Research Institution: The University of Tokyo
• Principal Investigator: 福田 裕穂 東京大学, 大学院・理学系研究科, 教授 (10165293)
• Keywords: 成長生理 / 植物 / 発生・分化

Research Abstract

後生木部道管のマスター遺伝子VND6をエストロゲン誘導型にしてシロイヌナズナ培養細胞に導入し、形質転換ラインを作成した。この実験系を用いてマイクロアレイをした結果、VND6は細胞死関連の遺伝子の発現を直接に制御している可能性が示唆された。詳細なプロモーター解析の結果、VND6は細胞死関連の遺伝子上にあるTERE配列に結合し、これらの遺伝子の発現を誘導することが明らかになった。この結果から、植物の細胞死の誘導機構は、プログラム細胞死に共通の仕組みがあり、転写・翻訳を介さずに細胞死シグナルの引き金を引くという動物の仕組みとは大きく異なっていることが明らかとなった。
VND6-誘導木部分化実験系を用いてライブイメージをとることで、壁孔タイプの二次細胞壁パターン形成の分子機構を解析した。まず、二次細胞壁パターン形成に関与する新規微小管付随タンパク質MIDD1を単離し、この機能をVND6-誘導木部分化実験系を用いて解析した。MIDD1は2つのコイルドコイルドメインをもつだけのタンパク質であり、N端のコイルドコイルドメインで微小管と、C末のコイルドコイルドメインで将来の壁孔形成部分の細胞膜と結合した。このタンパク質は壁孔部分で微小管の+端に結合し、微小管を破壊することで、微小管不在領域をつくり、その結果に次壁沈着が起こらないことが明らかとなった。実際、このタンパク質を減少させると、壁孔のない二次細胞壁の形成が誘導された。
維管束の継続的形成に必要なVAN3-ARF-GAPとVND7/GNOM-ARF-GEFの細胞内機能についてさらに解析した。その結果、VAN3-ARF-GAPとGNOM-ARF-GEFは一緒に、PIN1タンパク質などのエンドサイトーシスに関与していることが明らかとなった。

Research Products

• [Journal Article] ANGUSTIFOLIA3 plays roles in adaxial/abaxial patterning and growth in leaf morphogenesis (2011)
  • Author(s): Horiguchi, G., Nakayama, H., Ishikawa N., Kubo, M., Demura, T., Fukuda, H., Tsukaya, H
  • Journal Title: Plant Cell Physiol Volume: 52 Pages: 112-124
  • DOI: DOI:10.1093/pcp/pcq178
  • Peer Reviewed
• [Journal Article] CLE peptides can negatively regulate protoxylem vessel formation via cytokinin signaling (2011)
  • Author(s): Kondo, Y., Hirakawa, Y., Kieber, J., Fukuda, H
  • Journal Title: Plant Cell Physiol Volume: 52 Pages: 37-48
  • DOI: DOI:10.1093/pcp/pcq129
  • Peer Reviewed
• [Journal Article] Mitogen-Activated Protein Kinase regulated by the CLAVATA receptors contributes to the shoot apical meristem homeostasis (2011)
  • Author(s): Betsuyaku, S., Takahashi, F., Kinoshita, A., Miwa, H., Shinozaki, K., Fukuda, H., Sawa, S
  • Journal Title: Plant Cell Physiol Volume: 52 Pages: 14-29
  • DOI: DOI: 10.1093/pcp/pcq157
  • Peer Reviewed
• [Journal Article] Dynamics of Arabidopsis SUN proteins during mitosis and their involvement in nuclear shaping (2011)
  • Author(s): Oda, Y., H.Fukuda
  • Journal Title: Plant J. Volume: 66 Pages: 629-641
  • Peer Reviewed
• [Journal Article] CKH1/EER4 gene encoding a TAF12-like protein negatively regulates cytokinin sensitivity in Arabidopsis thaliana (2011)
  • Author(s): Kubo, M., Furuta, K., Demura, T., Fukuda, F., Liu, Y.-G., Shibata, S., Kakimoto, T.
  • Journal Title: Plant Cell Physiol. Volume: 52 Pages: 618-637
  • Peer Reviewed
• [Journal Article] Establishment and maintenance of vascular cell communities through local signaling (2011)
  • Author(s): Hirakawa Y., Kondo, Y., Fukuda, H.
  • Journal Title: Curr.Opin.Plant Blol. Volume: 14 Pages: 17-23
  • Peer Reviewed
• [Journal Article] Arabidopsis VASCULAR-RELATED NAC-DOMAIN6 directly regulates genes that govern programmed cell death and secondary wall formation in a coordinated way during xylem differentiation (2010)
  • Author(s): Ohashi-Ito, K., Oda, Y., Fukuda, H
  • Journal Title: Plant Cell Volume: 22 Pages: 3461-3473
  • URL: http://dx.doi.org/10.1105/tpc.110.075036
  • Peer Reviewed
• [Journal Article] TDIF peptide signaling regulates vascular stem cell proliferation via the wox4 homeobox gene in Arabidopsis (2010)
  • Author(s): Hirakawa Y., Kondo, Y., Fukuda, H
  • Journal Title: Plant Cell Volume: 22 Pages: 2618-2629
  • URL: http://dx.doi.org/10.1105/tpc.110.076083
  • Peer Reviewed
• [Journal Article] A receptor-like kinase, KLAVIER, mediates systemic regulation of nodulation and non-symbiotic shoot development in Lotus japonicus (2010)
  • Author(s): Miyazawa, H., Oka-Kira, E., Sato, N., Takahashi, H., Wu, G.J., Sato, S., Hayashi, M., Betsuyaku, S., Nakazono, M., Tabata, S., Harada, Y., Sawa, S., Fukuda, H., Kawaguchi, M
  • Journal Title: Development Volume: 137 Pages: 4317-4325
  • DOI: DOI:10.1242/dev.058891
  • Peer Reviewed
• [Journal Article] VND-INTERACTING2, a NAC Domain Protein, Negatively Regulates Xylem Vessel Formation in Arabidopsis (2010)
  • Author(s): Yamaguchi, M., Ohtania, M., Mitsuda, N., Kubo, M., Ohme-Takagi, M., Fukuda, H., Demura, T.
  • Journal Title: Plant Cell Volume: 22 Pages: 1249-1263
  • Peer Reviewed
• [Journal Article] Wood cell-wall structure requires local 2D-microtubule disassembly by a novel plasma membrane-anchored microtubule-associated protein (2010)
  • Author(s): Oda, Y., Iida, Y., Kondo, Y., Fukuda H.
  • Journal Title: Curr.Biol. Volume: 20 Pages: 1197-1202
  • Peer Reviewed
• [Journal Article] COE1, an LRR-RLK responsible for commissral vein pattern formation in rice (2010)
  • Author(s): Sakaguchi, J., Itoh, J.-I., Ito, Y., Nakamura, A., Fukuda, H., Sawa, S.
  • Journal Title: Plant Journal Volume: 63 Pages: 405-416
  • Peer Reviewed
• [Journal Article] RPK2/TOAD2 is an essential receptor-like kinase transmitting the CLV3 signal in Arabidopsis (2010)
  • Author(s): Kinoshita, A., Betsuyaku, S., Osakabe, Y., Mizuno, S., Nagawa, S., Stahl, Y., Simon, R., Yamaguchi-Shinozaki, K., Fukuda, H., Sawa, S.
  • Journal Title: Development Volume: 137 Pages: 3911-3920
  • Peer Reviewed
• [Journal Article] ADP-ribosylation factor machinery mediates endocytosis in plant cells (2010)
  • Author(s): Naramoto, S., Kleine-Vehn, J., Robert, S., Fujimoto, M., Dainobu, T., Paciorek, T., Ueda, T., Nakano, A., Van Montagu, M.C.E., Fukuda, H., Friml, J.
  • Journal Title: Proc.Natl.Acad.Sci., USA Volume: 107 Pages: 21890-21895
  • Peer Reviewed
• [Journal Article] Regulation of vascular development by CLE peptide-receptor systems (2010)
  • Author(s): Hirakawa Y., Kondo, Y., Fukuda, H.
  • Journal Title: J.Int.Plant Biol. Volume: 52 Pages: 8-16
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of vascular cell fates (2010)
  • Author(s): Ohashi-Ito, K., Fukuda, H.
  • Journal Title: Curr.Opin.Plant Biol. Volume: 13 Pages: 670-676
  • Peer Reviewed
• [Presentation] Maintenance of vascular stem cell activity by TDIF Peptide (2010)
  • Author(s): Fukuda, H., Kondo, Y., Hirakawa, Y.
  • Organizer: Plant Science Communications 2010, 2^<nd> NIBB-MPIPZ symposium
  • Place of Presentation: Okazaki Conference Center, Japan
  • Year and Date: 20101116-20101118
• [Presentation] A novel membrane-associated protein regulating secondary wall patterns in an Arabidopsis cell culture harboring inducible VND6 (2010)
  • Author(s): Fukuda, H., Ohashi-Ito, K., Oda, Y
  • Organizer: Cold Spring Harbor Asia Symposium "From Plant Biology to Crop Biotechnology"
  • Place of Presentation: CSH Asia, Suzhou, China
  • Year and Date: 20101025-29
• [Presentation] COE1, an LRR-RLK responsible for commissural vein pattern formation in rice (2010)
  • Author(s): Sakaguchi, J., Itoh, J.-L., Ito, Y., Nakamura, A., Sawa, S., Fukuda, H.
  • Organizer: Cold Spring Harbor Asia Symposium "From Plant Biology to Crop Biotechnology"
  • Place of Presentation: CSH Asia, Suzhou, China
  • Year and Date: 20101025-20101029
• [Presentation] CLE peptides govern vascular stem cell fates (2010)
  • Author(s): Fukuda, H., Kondo, Y., Hirakawa, Y.
  • Organizer: 8th International Conference on Plant Biology Frontiers : Cells and Signals
  • Place of Presentation: Wuyishan Yeohwa Resort, Wuyishan City, Fujian, China
  • Year and Date: 20100923-20100929
• [Presentation] 細胞膜ドメインに局在する新規微小管結合タンパク質MIDD1が二次細胞壁パターンを制御する (2010)
  • Author(s): 小田祥久、福田裕穂
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学(愛知県春日井市)
  • Year and Date: 20100909-20100911
• [Presentation] CLEペプチドの成熟に関与するペプチダーゼの解析 (2010)
  • Author(s): 玉置貴之、別役重之、深尾陽一朗、藤原正幸、福田裕穂、澤進一郎
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学(愛知県春日井市)
  • Year and Date: 20100909-20100911
• [Presentation] CLEペプチドとサイトカイニンによる原生木部配置パターンの制御 (2010)
  • Author(s): 近藤侑貴、平川有宇樹、福田裕穂
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学(愛知県春日井市)
  • Year and Date: 20100909-20100911
• [Presentation] 維管束形成におけるTDIFシグナルによる幹細胞機能の制御 (2010)
  • Author(s): 平川有宇樹、近藤侑貴、福田裕穂
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学(愛知県春日井市)
  • Year and Date: 20100909-20100911
• [Presentation] 維管束形成に関わる新規細胞間相互作用因子CLE46の解析 (2010)
  • Author(s): 大槻和弘、遠藤暁詩、伊藤容子、伊藤(大橋)恭子、福田裕穂
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大芦(愛知県春日井市)
  • Year and Date: 20100909-20100911
• [Presentation] Clavata2(clv2)突然変異体のエンハンサースクリーニング (2010)
  • Author(s): 山田昌史, 福田裕穂, 澤進一郎
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学(愛知県春日井市)
  • Year and Date: 20100909-20100911
• [Presentation] Cellular and transcriptome analyses of xylem formation with Arabidopsis cell culture systems harboring inducible VND6 and SND1 (2010)
  • Author(s): Fukuda, H., Oda, Y., Ohashi-Ito, K
  • Organizer: Plant Vascular Biology 2010
  • Place of Presentation: Ohio State University, Ohio, USA
  • Year and Date: 20100723-28
• [Presentation] CLE peptides regulating vascular stem cell fates (2010)
  • Author(s): Fukuda, H., Kondo, Y., Hirakawa, Y.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Pacifico Yokohama, Japan
  • Year and Date: 20100606-20100610
• [Presentation] Screening of the CLV2 enhancer mutants (2010)
  • Author(s): Yamada, M., Fukuda, H., Sawa, S.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Pacifico Yokohama, Japan
  • Year and Date: 20100606-20100610
• [Presentation] Analysis of downstream genes regulated by master regulators of vascular differentiation (2010)
  • Author(s): Ohashi-Ito, K., Oda, Y., Fukuda, H.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Pacifico Yokohama, Japan
  • Year and Date: 20100606-20100610
• [Presentation] Regulation of auxin transport dependent leaf vascular continuity by VAN3 ARFGAP (2010)
  • Author(s): Naramoto, S, Koizumi, K., Ueda, T., Nakano, A., Fukuda, H., Friml, J.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Pacifio Yokohama, Japan
  • Year and Date: 20100606-20100610
• [Presentation] Molecular dissection of CLV3 signaling (2010)
  • Author(s): Betsuyaku, S., Kinoshita, A., Fujiwara, M., Fukao, Y., Kuromori, T., Shinozaki, K., Suzuki, A., Takahashi, H., Fukuda, H., Sawa, S.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Pacifico Yokohama, Japan
  • Year and Date: 20100606-20100610
• [Presentation] Genetic analysis of the CLV signaling pathway in the SAM (2010)
  • Author(s): Kinoshita, A., Betsuyaku, S., Yamada, M., Fukuda, H., Sawa, S.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Pacifico Yokohama, Japan
  • Year and Date: 20100606-20100610
• [Presentation] TDIF peptide signaling regulates vascular stem cell proliferation via the WOX4 homeobox gene in Arabidopsis (2010)
  • Author(s): Hirakawa, Y., Kondo, Y., Fukuda, H.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Pacifico Yokohama, Japan
  • Year and Date: 20100606-20100610
• [Presentation] Wood cell-wall structure requires local 2d-microtubule disassembly by a novel plasma membrane-anchored microtubule-associated protein (2010)
  • Author(s): Oda, Y., Fukuda, H.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Pacifico Yokohama, Japan
  • Year and Date: 20100606-20100610
• [Presentation] VND-INTERACTING2, a NAC domain transcription factor, negatively regulates xylem vessel formation in Arabidopsis (2010)
  • Author(s): Yamaguchi, M., Ohtani, M., Mitsuda, N., Kubo, M.Ohme-Takagi, M., Fukuda, H., Demura, T.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Pacifico Yokohama, Japan
  • Year and Date: 20100606-20100610
• [Presentation] CLE peptides governing vascular stem cell fates (2010)
  • Author(s): Fukuda, H., Kondo, Y., Hirakawa, Y.
  • Organizer: A seminar in The University of Michigan
  • Place of Presentation: Ann Arbor, Michigan, USA
  • Year and Date: 2010-07-29
• [Book] Plant tissue cultures. In "Methods in Molecular Biology : The Plant Cell Wall", vol.715(eds.Z.A.Popper) (2011)
  • Author(s): Karkonen, A., Santanen, A., Iwamoto K., Fukuda, H.
  • Total Pages: 1-20
  • Publisher: Springer
• [Book] Plant tracheary elements. In "Encyclopedia of Life Sciences" DOI:10.1002/9780470015902.a0001814.pub2, Wiley Online Library (2010)
  • Author(s): Fukuda, H.
  • Publisher: John Wiley & Sons, Inc.
  • DOI: 10.1002/9780470015902.a0001814.pub2
• [Book] CLEペプチドによる維管束形成の制御、植物のシグナル伝達-分子と応答(柿本、高山、福田、松岡編) (2010)
  • Author(s): 平川有宇樹、近藤侑貴、福田裕穂
  • Total Pages: 172-177
  • Publisher: 共立出版
• [Book] 植物寄生性線虫がもつ植物ペプチドホルモン様遺伝子の機能とは?,遺伝,vol.64 (2010)
  • Author(s): 別役重之、澤進一郎、福田裕穂
  • Total Pages: 52-59
  • Publisher: エヌ・エス・テイ
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/seigyo/lab.html