Development of vascular bundles as a center of the signaling transport

2012 Fiscal Year Final Research Report

• Project Area: Plant regulatory systems that control developmental interactions between meristems and lateral organs
• Project/Area Number: 19060009
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: FUKUDA Hiroo 東京大学, 大学院・理学系研究科, 教授 (10165293)
• Co-Investigator(Renkei-kenkyūsha): ITO Kyoko 東京大学, 大学院・理学系研究科, 助教 (90451830)
• Project Period (FY): 2007 – 2012
• Keywords: 成長生理 / 植物 / 発生・分化

Research Abstract

We have identified various factors involved in vascular tissue formation. In this study, we attempted to understand the function of these factors and their interrelationship at a molecular level, in view of following three aspects: (1) Regulation of vascular meristem formation by intercellular CLE peptides, (2) Regulation of vascular tissue formation by transcription factors, (3) Regulation of vascular pattern formation by spatial control of subcellular components. As a result, (1) we found intercellular and intracellular signaling pathways of CLE peptides regulating vascular meristem formation. In addition, we also indicated that TDIF functions in not only vascular but also axillary meristem formation. (2) Machinery of transcriptional regulation by VND6 via the TERE sequence was uncovered. Moreover, it was shown that bHLH transcription factors act at the initiation of vascular differentiation probably by inducing auxin transport. (3) We identified VAN3-ARF-GAP and MIDD1 as crucial factors governing cell polarity. In conclusion, we succeeded in demonstrating that vascular stem cell fates are determined by a specific transcription factor(s) regulated by a CLE peptide-dependent intracellular signaling pathway composed of a receptor kinase and a cellular kinase.

Research Products

• [Journal Article] Auxin-associated initiation of vascular cell differentiation by LONESOME HIGHWAY (2013)
  • Author(s): Ohashi-Ito, K., Obuchi, M., Kojima, M., Sakakibara, H., Fukuda, H
  • Journal Title: Development Volume: 140 Pages: 765-769
  • DOI: DOI:10.1242/dev.087924
  • Peer Reviewed
• [Journal Article] An atypical bHLH transcription factor regulates early xylem development downstream of auxin (2013)
  • Author(s): Ohashi-Ito, K., Matsukawa, M., Fukuda, H
  • Journal Title: Plant Cell Physiol Volume: 54 Pages: 398-405
  • DOI: DOI:10.1093/pcp/pct013
  • Peer Reviewed
• [Journal Article] The proteasome is responsible for caspase-3-like activity during xylem development (2012)
  • Author(s): Han, J. J., Lin, W., Oda, Y., Cui, K. M., Fukuda, H., He, X.Q
  • Journal Title: Plant J Volume: 72 Pages: 129-41
  • DOI: DOI: 10.1111/j.1365-313X.2012.05070.x
  • Peer Reviewed
• [Journal Article] Initiation of cell wall pattern by a Rho- and microtubule-driven symmetry breaking (2012)
  • Author(s): Oda, Y., Fukuda, H
  • Journal Title: Science Volume: 337 Pages: 1333-1336
  • DOI: DOI:10.1126/science.1222597
  • Peer Reviewed
• [Journal Article] Secondary cell wall patterning during xylem differentiation (2012)
  • Author(s): Oda, Y., and Fukuda, H
  • Journal Title: Curr. Opin. Plant Biol Volume: 15 Pages: 38-44
  • DOI: DOI:10.1016/j.pbi.2011.10.005
  • Peer Reviewed
• [Journal Article] Establishment and maintenance of vascular cell communities through local signaling (2011)
  • Author(s): Hirakawa Y., Kondo, Y., Fukuda, H
  • Journal Title: Curr. Opin. Plant Biol Volume: 14 Pages: 17-23
  • DOI: DOI:10.1016/j.pbi.2010.09.011
  • Peer Reviewed
• [Journal Article] Dynamics of Arabidopsis SUN proteins during mitosis and involvement in nuclear shaping (2011)
  • Author(s): Oda, Y., Fukuda, H
  • Journal Title: Plant J Volume: 66 Pages: 629-641
  • DOI: DOI:10.1111/j.1365-313X.2011.04523.x
  • Peer Reviewed
• [Journal Article] ANGUSTIFOLIA3 plays roles in adaxial/abaxial patterning and growth in leaf morphogenesis (2011)
  • Author(s): Horiguchi, G., Nakayama, H., Ishikawa N., Kubo, M., Demura, T., Fukuda, H., Tsukaya, H
  • Journal Title: Plant Cell Physiol Volume: 52 Pages: 112-124
  • DOI: DOI:10.1093/pcp/pcq178
  • Peer Reviewed
• [Journal Article] CLE peptides can negatively regulate protoxylem vessel formation via cytokinin signaling (2011)
  • Author(s): Kondo, Y., Hirakawa, Y., Kieber, J., Fukuda, H
  • Journal Title: Plant Cell Physiol Volume: 52 Pages: 37-48
  • DOI: DOI:10.1093/pcp/pcq129
  • Peer Reviewed
• [Journal Article] Mitogen-Activated Protein Kinase regulated by the CLAVATA receptors contributes to the shoot apical meristem homeostasis (2011)
  • Author(s): Betsuyaku, S., Takahashi, F., Kinoshita, A., Miwa, H., Shinozaki, K., Fukuda, H., Sawa, S
  • Journal Title: Plant Cell Physiol Volume: 52 Pages: 14-29
  • DOI: DOI: 10.1093/pcp/pcq157
  • Peer Reviewed
• [Journal Article] Wood cell-wall structure requires local 2D-microtubule disassembly by a novel plasma membrane-anchored microtubuleassociated protein (2010)
  • Author(s): Oda, Y., Iida, Y., Kondo, Y., Fukuda, H
  • Journal Title: Curr. Biol Volume: 20 Pages: 1197-1202
  • DOI: DOI:10.1016/j.cub.2010.05.038
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of vascular cell fates (2010)
  • Author(s): Ohashi-Ito, K., Fukuda, H
  • Journal Title: Curr. Opin. Plant Biol Volume: 13 Pages: 670-676
  • URL: http://dx.doi.org/10.1016/j.pbi.2010.08.011
  • Peer Reviewed
• [Journal Article] COE1, an LRR-RLK responsible for commissral vein pattern formation in rice (2010)
  • Author(s): Sakaguchi, J., Itoh, J.-I., Ito, Y., Nakamura, A., Fukuda, H., Sawa, S
  • Journal Title: Plant J Volume: 63 Pages: 405-416
  • DOI: DOI:10.1111/j.1365-313X.2010.04250.x
  • Peer Reviewed
• [Journal Article] Arabidopsis VASCULAR-RELATED NAC-DOMAIN6 directly regulates genes that govern programmed cell death and secondary wall formation in a coordinated way during xylem differentiation (2010)
  • Author(s): Ohashi-Ito, K., Oda, Y., Fukuda, H
  • Journal Title: Plant Cell Volume: 22 Pages: 3461-3473
  • URL: http://dx.doi.org/10.1105/tpc.110.075036
  • Peer Reviewed
• [Journal Article] TDIF peptide signaling regulates vascular stem cell proliferation via the wox4 homeobox gene in Arabidopsis (2010)
  • Author(s): Hirakawa Y., Kondo, Y., Fukuda, H
  • Journal Title: Plant Cell Volume: 22 Pages: 2618-2629
  • URL: http://dx.doi.org/10.1105/tpc.110.076083
  • Peer Reviewed
• [Journal Article] ADP-ribosylation factor (ARF), guanine nucleotide exchange factor (GEF), and GAPase-acticating protein (GAP) function in endocytosis of plant cells (2010)
  • Author(s): Naramoto, S., Kleine-Vehn, J., Robert, S., Fujimoto, M., Dainobu, T., Paciorek, T., Ueda, T., Nakano, A., Van Montagu, M. C. E., Fukuda, H., Friml, J
  • Journal Title: Proc. Natl. Acad. Sci., USA Volume: 107 Pages: 21890-21895
  • DOI: DOI:10.1073/pnas.1016260107
  • Peer Reviewed
• [Journal Article] A receptor-like kinase, KLAVIER, mediates systemic regulation of nodulation and non-symbiotic shoot development in Lotus japonicus (2010)
  • Author(s): Miyazawa, H., Oka-Kira, E., Sato, N., Takahashi, H., Wu, G.J., Sato, S., Hayashi, M., Betsuyaku, S., Nakazono, M., Tabata, S., Harada, Y., Sawa, S., Fukuda, H., Kawaguchi, M
  • Journal Title: Development Volume: 137 Pages: 4317-4325
  • DOI: DOI:10.1242/dev.058891
  • Peer Reviewed
• [Journal Article] RPK2 is an essential receptor-like kinase that transmits the CLV3 signal in Arabidopsis (2010)
  • Author(s): Kinoshita, A., Betsuyaku, S., Osakabe, Y., Mizuno, S., Nagawa, S., Stahl, Y., Simon, R., Yamaguchi-Shinozaki, K., Fukuda, H., Sawa, S
  • Journal Title: Development Volume: 137 Pages: 3911-3920
  • DOI: DOI:10.1242/dev.048199
  • Peer Reviewed
• [Journal Article] Involvement of phytosulfokine in the attenuation of stress response during the transdifferentiation of Zinnia mesophyll cells into tracheary elements (2009)
  • Author(s): Motose, H., Iwamoto, K., Endo, S., Demura, T., Sakagami, Y., Matsubayashi, Y., Moore, K. L., Fukuda, H
  • Journal Title: Plant Physiol Volume: 150 Pages: 437-447
  • DOI: DOI:org/10.1104/pp.109.135954
  • Peer Reviewed
• [Journal Article] Phosphoinositide-dependent regulation of VAN3 ARF-GAP localization and activity essential for vascular tissue continuity in plants (2009)
  • Author(s): Naramoto, S., Sawa, S., Koizumi, K., Uemura, T., Ueda, T., Friml, J., Nakano, A., Fukuda, H
  • Journal Title: Development Volume: 136 Pages: 1529-1538
  • DOI: DOI:10.1242/dev.030098
  • Peer Reviewed
• [Journal Article] Non-cell-autonomous control of vascular stem cell fates by a CLE peptide/receptor system (2008)
  • Author(s): Hirakawa Y., Shinohara, H., Kondo, Y., Inoue, A., Nakanomyo, I., Ogawa, M., Sawa, S., Ohashi-Ito, K., Matsubayashi, Y., Fukuda, H
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 105 Pages: 115208-15213
  • DOI: DOI:10.1073/pnas.0808444105
  • Peer Reviewed
• [Journal Article] VASCULAR-RELATED NAC-DOMAIN7 is involved in differentiation of all types of xylem vessels in Arabidopsis roots and shoots (2008)
  • Author(s): Yamaguchi, M., Kubo, M., Fukuda, H., Demura, T
  • Journal Title: Plant J Volume: 55 Pages: 652-664
  • DOI: DOI:10.1111/j.1365-313X.2008.03533.x
  • Peer Reviewed
• [Journal Article] Cell differentiation in the longitudinal veins and formation of commissural veins in rice (Oryza sativa) and maize (Zea mays) (2008)
  • Author(s): Sakaguchi, J., Fukuda, H
  • Journal Title: J. Plant Res Volume: 121 Pages: 593-602
  • DOI: DOI:10.1007/s10265-008-0189-1
  • Peer Reviewed
• [Journal Article] Transient transformation and RNA silencing in Zinnia tracheary element differentiating cell cultures (2008)
  • Author(s): Endo, S., Pesquet, E., Tashiro, G., Kuriyama, H., Goffner, D., Fukuda, H., Demura, T
  • Journal Title: Plant J Volume: 53 Pages: 864-875
  • DOI: DOI:10.1111/j.1365-313X.2007.03377.x
  • Peer Reviewed
• [Presentation] Regulation of microtubule organization directing spatial patterning of wall assembly during xylem cell formation in Arabidopsis. Gordon Conference (2012)
  • Author(s): Fukuda, H. and Oda, Y
  • Organizer: Plant & Microbial Cytoskeleton
  • Place of Presentation: Andover, NH, USA
  • Year and Date: 20120812-17
• [Presentation] Inter- and intra-signaling regulating vascular stem cell maintenance (2012)
  • Author(s): Fukuda, H, Tamaki, T., Hirakawa, Y., Kondo, Y
  • Organizer: Plant Vascular Development, 2012
  • Place of Presentation: Vienna, Austria
  • Year and Date: 20120701-02
• [Presentation] A mechanism determining cell wall pattern. International Symposium of Plant Science (2012)
  • Author(s): Fukuda, H. and Oda, Y
  • Organizer: Plant Science for the future
  • Place of Presentation: 理化学研究所横浜研究所、鶴見
  • Year and Date: 2012-11-20
• [Presentation] A novel membrane-associated protein regulating secondary wall patterns in an Arabidopsis cell culture harboring inducible VND6 (2010)
  • Author(s): Fukuda, H., Ohashi-Ito, K., Oda, Y
  • Organizer: Cold Spring Harbor Asia Symposium "From Plant Biology to Crop Biotechnology"
  • Place of Presentation: CSH Asia, Suzhou, China
  • Year and Date: 20101025-29
• [Presentation] Cellular and transcriptome analyses of xylem formation with Arabidopsis cell culture systems harboring inducible VND6 and SND1 (2010)
  • Author(s): Fukuda, H., Oda, Y., Ohashi-Ito, K
  • Organizer: Plant Vascular Biology 2010
  • Place of Presentation: Ohio State University, Ohio, USA
  • Year and Date: 20100723-28
• [Presentation] CLE peptides regulating vascular stem cell fates (2010)
  • Author(s): Fukuda, H., Kondo, Y., Hirakawa, Y
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20100606-10
• [Presentation] Peptide signals governing vascular stem cell fates (2010)
  • Author(s): Fukuda, H. Kondo, Y., Hirakawa, Y
  • Organizer: Keystone symposia 2010
  • Place of Presentation: Granlibakken Resort, Tahoe City, California, USA
  • Year and Date: 20100314-19
• [Presentation] Crosstalk between xylem and phloem production through a peptide (2009)
  • Author(s): Fukuda, H. Kondo, Y., Hirakawa, Y
  • Organizer: Plant Vascular Biology and Agriculture 2009
  • Place of Presentation: Chongqing, China
  • Year and Date: 2009-06-22
• [Presentation] Cell-cell communication governing vascular tissue organization (2008)
  • Author(s): Fukuda, H
  • Organizer: The XVI Congress of the Federation of European Societies of Plant Biology
  • Place of Presentation: Tampere, Finland
  • Year and Date: 2008-08-21
• [Book] Peptide regulating vascular development. In "Plant Signaling Peptides" (eds. H. R. Irving and C. Gehring), p.59-75 (2012)
  • Author(s): Fukuda, H
  • Total Pages: 284
  • Publisher: Springer
  • URL: ISBN: 978-3-642-27602-6 (Print), 978-3-642-27603-3 (Online)
• [Book] Plant tissue cultures. In "Methods in Molecular Biology: The Plant Cell Wall"(eds. Z. A. Popper), vol.715, p.1-20 (2011)
  • Author(s): Karkonen, A., Santanen, A., Iwamoto K., Fukuda, H
  • Total Pages: 310
  • Publisher: Springer
  • URL: DOI: 10.1007/978-1-61779-008-9_1, ISBN 9781617790072
• [Book] Plant tracheary elements. In "Encyclopedia of Life Sciences", Wiley Online Library (2010)
  • Author(s): Fukuda, H
  • Publisher: Wiley-Blackwell
  • URL: DOI:10.1002/9780470015902.a0001814.pub2
• [Book] CLE ペプチドによる維管束形成の制御、植物のシグナル伝達 - 分子と応答(柿本、高山、福田、松岡編), p.172-177 (2010)
  • Author(s): 平川有宇樹、近藤侑貴、福田裕穂
  • Total Pages: 238
  • Publisher: 共立出版
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/seigyo/lab.html