Identification of ligand-receptor pairs in plants

2009 Fiscal Year Self-evaluation Report

• Project Area: Plant regulatory systems that control developmental interactions between meristems and lateral organs
• Project/Area Number: 19060010
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: National Institute for Basic Biology (2010); Nagoya University (2007-2009)
• Principal Investigator: MATSUBAYASHI Yoshikatsu Nagoya University, 大学院・生命農学研究科, 准教授 (00313974)
• Project Period (FY): 2007 – 2012
• Keywords: ペプチドホルモン / 受容体 / 翻訳後修飾 / シロイヌナズナ

Research Abstract

分泌型ペプチドおよび特異的受容体を介した情報伝達は,多細胞生物の形態形成における細胞間情報伝達に必須のメカニズムであるが、植物ではその研究例は依然として少ない.本研究では,in silico遺伝子スクリーニングによる新規ペプチドホルモン候補遺伝子の探索や分泌型ペプチドミクス,LRR型受容体キナーゼを網羅的に発現した受容体ライブラリの構築などを組み合わせて新たなペプチドホルモンやその受容体を同定する.これらの解析から得られる分子群を基軸として、植物の形態形成を支える新たな情報統御系の発見を目指す.

Research Products

• [Journal Article] Identification of tyrosylprotein sulfotransferase in Arabidopsis. (2009)
  • Author(s): Komori, R., Amano, Y., Ogawa-Ohnishi, M., Matsubayashi, Y.
  • Journal Title: Proc Natl Acad Sci USA 106 Pages: 15067-15072
• [Journal Article] A glycopeptide regulating stem cell fate in Arabidopsis thaliana. (2009)
  • Author(s): Ohyama, K., Shinohara, H., Ogawa-Ohnishi, M., Matsubayashi, Y.
  • Journal Title: Nature Chem Biol 5 Pages: 578-580
• [Journal Article] Arabidopsis CLV3 peptide directly binds CLV1 ectodomain. (2008)
  • Author(s): Ogawa, M., Shinohara, H., Sakagami, Y., Matsubayashi, Y.
  • Journal Title: Science 319 Pages: 294
• [Journal Article] Tyrosine-sulfated glycopeptide involved in cellular proliferation and expansion in Arabidopsis. (2007)
  • Author(s): Amano, Y., Tsubouchi, H., Shinohara, H., Ogawa, M., Matsubayashi, Y.
  • Journal Title: Proc Natl Acad Sci USA 104 Pages: 18333-18338
• [Journal Article] Functional immobilization of plant receptor-like kinase onto microbeads towards receptor array construction and receptor-based ligand fishing. (2007)
  • Author(s): Shinohara, H., Matsubayashi, Y.
  • Journal Title: Plant J 52 Pages: 175-184
• [Presentation] ペプチドリガンド-受容体ペアの解析から探る植物のかたちづくり (2009)
  • Author(s): 松林嘉克
  • Organizer: 第82回日本生化学会大会トップランナーズレクチャー
  • Place of Presentation: 神戸
  • Year and Date: 20091200
• [Presentation] Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis. (2008)
  • Author(s): Matsubayashi, Y., Ohyama, K., Ogawa, M.
  • Organizer: Japan-Korea Symposium - Plant Growth and Signal Transduction.
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20081000
• [Presentation] Physiological functions of two structurally distinct tyrosine-sulfated peptides in Arabidopsis growth and development. (2007)
  • Author(s): Matsubayashi Y.
  • Organizer: 18th International Conference on Arabidopsis Research.
  • Place of Presentation: Beijing, China
  • Year and Date: 20070700
• [Book] 第9章ペプチドホルモン.新しい植物ホルモンの科学 (2010)
  • Author(s): 松林嘉克
  • Total Pages: 154-168
  • Publisher: 講談社
• [Book] ペプチペプチドリガンド-受容体ペアの解析から探る植物のかたちづくり.蛋白質核酸酵素 (2009)
  • Author(s): 松林嘉克
• [Book] リガンド-受容体ペアの解析から探る植物の細胞間シグナリング.化学と生物 (2008)
  • Author(s): 松林嘉克, 篠原秀文
  • Total Pages: 588-590