2023 Fiscal Year Final Research Report

Post-Koch Ecology: The next-era microbial ecology that elucidates the super-terrestrial organism system

Planned Research

PDF

Project Area	Post-Koch Ecology: The next-era microbial ecology that elucidates the super-terrestrial organism system
Project/Area Number	19H05682
Research Category	Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
Allocation Type	Single-year Grants
Review Section	Complex systems
Research Institution	University of Tsukuba
Principal Investigator	Nomura Nobuhiko 筑波大学, 生命環境系, 教授 (60292520)
Co-Investigator(Kenkyū-buntansha)	豊福雅典筑波大学, 生命環境系, 准教授 (30644827)
Project Period (FY)	2019-06-28 – 2024-03-31
Keywords	微生物間相互作用 / バイオフィルム / イメージング解析 / メンブレンベシクル / 土壌
Outline of Final Research Achievements	By verifying bacterial interactions in real-world samples, we aim to establish a new technique for isolation and analysis of complex microorganisms. First, we attempted one-cell visualization of microbial interactions by CRIF using model bacteria to establish a non-destructive bacterial interaction system, and succeeded in detecting cells responding to bacterial signals in the interaction. Furthermore, by applying this method, we discovered a novel bacterial communication mechanism in field soil samples, which has not been reported before. We also focused on microscopic membrane vesicles (MVs), which transfer information between bacteria across genera and species in complex microbial systems. As a result, certain findings were obtained regarding the iron transport function and cell specificity of MVs in complex systems.
Free Research Field	応用微生物学、分子生物学
Academic Significance and Societal Importance of the Research Achievements	実圃場において、微生物間相互作用に関わる単離また解析法の基盤構築が出来た。本成果により、微生物相互作用における物質生産また作物（植物）の持続的生産の土壌づくりに新たな視点と方法が展開されることで、バイオものづくりや植物の炭素固定にも幅広く貢献することが期待される。