Reconstruction of totipotency using stem cells derived from preimplantation embryos

2023 Fiscal Year Final Research Report

• Project Area: Program of totipotency: From decoding to designing
• Project/Area Number: 19H05757
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kumamoto University (2022-2023); Tohoku University (2019-2021)
• Principal Investigator: Hiroaki Okae 熊本大学, 発生医学研究所, 教授 (10582695)
• Co-Investigator(Kenkyū-buntansha): 大日向 康秀 千葉大学, 大学院医学研究院, 講師 (70415107)
• Project Period (FY): 2019-06-28 – 2024-03-31
• Keywords: 全能性 / ES/iPS細胞 / 栄養膜幹細胞（TS細胞） / 原始内胚葉幹細胞（PrES細胞）

Outline of Final Research Achievements

This study aims to generate mouse artificial blastocysts and to derive stem cells from all cell lineages constituting a human blastocyst. In mice, we succeeded in establishing primitive endoderm stem cells (PrES cells) for the first time (Science 2022). In humans, we established a differentiation system from ES/iPS cells to TS cells and found that the primate-specific microRNA cluster C19MC is important in this process (Nat Commun 2022). Furthermore, we have established a new stem cell line from human yolk sac tissue.

Free Research Field

発生生物学

Academic Significance and Societal Importance of the Research Achievements

本研究では、マウスPrES細胞やヒト卵黄嚢幹細胞を樹立することに初めて成功した。さらに、マウスPrES細胞を用いた人工胚盤胞の再構築、ヒトTS細胞を用いた栄養膜系列への運命決定機構の解析などを通して、幹細胞を用いて哺乳類の初期発生を研究するための技術基盤を確立した。