2023 Fiscal Year Final Research Report
Regulation of physiological renal functions via dynamic cellular transports of biometals
| Project Area | Integrated Biometal Science: Research to Explore Dynamics of Metals in Cellular System |
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| Project/Area Number |
19H05770
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Complex systems
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| Research Institution | Tokushima Bunri University |
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Principal Investigator |
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| Project Period (FY) |
2019-06-28 – 2024-03-31
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| Keywords | 腎臓 / 生命金属 / 有害金属 / 近位尿細管 / 輸送 / 分布 / イメージング / シスプラチン |
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| Outline of Final Research Achievements |
This study aimed to elucidate the mechanisms of transport and toxicity of toxic metals in the proximal tubules, as well as the mechanisms of regulation of transport of bio-metals.
In vitro, we used proximal tubule region-specific cultured cells to clarify the transport of Cd and Mn and the mechanism of proximal tubular damage caused by cisplatin and Cd.On the other hand, in vivo, we utilized elemental imaging to examine how administered bio-metals are distributed in various regions of the kidney, focusing on Cd and Mn. We clarified how metal transport is affected by mutations that induce various diseases.
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| Free Research Field |
金属毒性学
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| Academic Significance and Societal Importance of the Research Achievements |
有害金属の引き起こす腎障害機構を解明するために腎臓細胞のtrans-well培養を用いて金属輸送と障害マーカーの誘導を解析し、実験動物を用いて金属の集積部位および障害を解析した結果より、腎臓という複雑な輸送システムを持つ臓器における有害金属の輸送と毒性発現との関係を明らかにできるシステムを構築できた。有害金属のための輸送体は存在せず、有害金属は必須生命金属のための輸送系を介して細胞に取り込まれ、排出される。したがって、有害金属の動態と毒性解明により、必須金属の輸送システムの調節機構と攪乱の機構の解明へと繋がる。
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