Comprehensive research of signal transduction of cell

2023 Fiscal Year Final Research Report

• Project Area: Information physics of living matters
• Project/Area Number: 19H05797
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: Osaka University
• Principal Investigator: test test 大阪大学, 大学院生命機能研究科, 教授 (80301216)
• Co-Investigator(Kenkyū-buntansha): 福岡 創 大阪大学, 大学院生命機能研究科, 准教授 (50447190) ; 蔡 栄淑 大阪大学, 大学院生命機能研究科, 助教 (40378716)
• Project Period (FY): 2019-06-28 – 2024-03-31
• Keywords: 走化性

Outline of Final Research Achievements

In this study, we focused on the bacterial repellent response and measured the localization of CheB to the cell poles, which is necessary for the adaptive phenomenon. We simultaneously measured the change in fluorescence intensity at the poles and the motor rotation after the administration of repellent substances, and estimated the dependence on the concentration of repellent substances, the correlation between the rotation and the localization, and the number of localized molecules. Furthermore, the response of mutants in which the CheB binding site of the receptor was deleted was also measured. In addition, we developed a system to simultaneously measure and analyze the responses of multiple bacteria to eliminate the variation among experiments. As a result, the variability was clearly reduced compared to individual measurements. We are currently investigating whether this remaining variability is unique to E. coli or to the experimental conditions.

Free Research Field

生物物理学

Academic Significance and Societal Importance of the Research Achievements

忌避応答に対する応答は，誘因応答と呼ばれ，よりよい環境を目指す応答であり，今までの走化性応答のほとんどの研究は誘因応答であった．しかしながら，忌避応答は，単に誘因応答の逆反応ととらえられてきた．しかしながら，生体の生存に必須なのはむしろ忌避応答であり，忌避応答の欠如は，死，に直結する．本研究においては，忌避応答のメカニズムを回転と局在の同時計測による定量的な研究の第一歩となる．