Molecular analysis of germinal granules/nuage in the mouse germline

2010 Fiscal Year Self-evaluation Report

• Project Area: The germline: its developmental cycle and epigenome network
• Project/Area Number: 20062004
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University
• Principal Investigator: NAKATSUJI Norio Kyoto University, 物質-細胞統合システム拠点, 教授 (80237312)
• Project Period (FY): 2008 – 2012
• Keywords: 発生・分化 / 遺伝学 / 遺伝子 / 動物 / バイオテクノロジー

Research Abstract

生殖細胞はゲノム情報を次世代に伝達し、個体発生の起点となる細胞系譜である。その発生分化プロセスではゲノム再プログラム化、個体発生能の獲得維持、DNA組換え等重要な生命現象が起こる。一方、生殖細胞に顕著に観察される構造的特徴に生殖顆粒RNPが挙げられる。同構造は世代、種を越えて広く生殖細胞に観察される事から生殖系列の特性に重要な役割を担うと考えられる。しかし生殖顆粒の機能研究は特に哺乳類において殆ど進んでいない。研究代表者らは哺乳類生殖顆粒の構成蛋白質群をコードするTdrd遺伝子ファミリーの同定、遺伝子改変マウス作成、表現型解析を行い、生殖顆粒の形成異常を示す複数の変異マウスを得た。本研究計画はこれら生殖顆粒の変異系統を実験材料の基盤として包括的なオミクス解析を重点に進め、生殖顆粒の生成異常に伴う細胞内の分子経路の特異的変動の解明を目指す。生殖顆粒の分子レベルでの機能研究を発展、展開する事で、同構造が担う重要な生物学的機能を明らかにすると共に、生体内に存在する新たな分子システムの同定を目指す。

Research Products

• [Journal Article] MVH in piRNA processing and gene silencing of retrotransposons. (2010)
  • Author(s): Kuramochi-Miyagawa S, Watanabe T, Gotoh K, Takamatsu K, Chuma S, Kojima-Kita K, Shiromoto Y, Asada N, Toyoda A, Fujiyama A, Totoki Y, Shibata T, Kimura T, Nakatsuji N, Noce T, Sasaki H, Nakano T
  • Journal Title: Genes Dev. 24 Pages: 887-892
  • Peer Reviewed
• [Journal Article] Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile. (2009)
  • Author(s): Reuter, M, Chuma, S, Tanaka, T, Franz, T, Stark, A, Pillai, RS
  • Journal Title: Nat.Struct.Mol.Biol. 16 Pages: 639-646
  • Peer Reviewed
• [Journal Article] The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline. (2009)
  • Author(s): Shoji, M, Tanaka, T, Hosokawa, M, Reuter, M, Stark, A, Kato, Y, Kondoh, G, Okawa, K, Chujo, T, Suzuki, T, Hata, K, Martin, SL, Noce, T, Kuramochi-Miyagawa, S, Nakano, T, Sasaki, H, Pillai, RS, Nakatsuji, N, Chuma, S
  • Journal Title: Dev.Cell. 17 Pages: 775-787
  • Peer Reviewed
• [Journal Article] Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members. (2009)
  • Author(s): Vagin, VV, Wohlschlegel, J, Qu, J, Jonsson, Z, Huang, X, Chuma, S, Girard, A, Sachidanandam, R, Hannon, GJ, Aravin, AA
  • Journal Title: Genes Dev. 23 Pages: 1749-1762
  • Peer Reviewed
• [Journal Article] Homing of mouse spermatogonial stem cells to germline niche depends on beta1-integrin. (2008)
  • Author(s): Kanatsu-Shinohara, M, Takehashi, M, Takashima, S, Lee, J, Morimoto, H, Chuma, S, Raducanu, A, Nakatsuji, N, Fassler, R, Shinohara, T
  • Journal Title: Cell Stem Cell. 3 Pages: 533-542
  • Peer Reviewed
• [Presentation] The roles of tudor genes in mammalian spermatogenesis. (2009)
  • Author(s): Takashi Tanaka, Mihoko Hosokawa, Norio Nakatsuji, Shinichiro Chuma
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-20091212
• [Presentation] Mammalian germ cells and germinal granules/nuage. (2008)
  • Author(s): Shinichiro Chuma
  • Organizer: Germ cell-soma interactions in gonadal development and germ cell tumors (workshops " current trends in biomedicine ")
  • Place of Presentation: Baeza, Spain
  • Year and Date: 20081020-20081022
• [Presentation] Role of TDRD9 in male meiosis and regulatory pathway of transposon RNA and DNA methylation. (2008)
  • Author(s): Shoji, M, Tanaka, T, Hosokawa, M, Kitamura, K, Kato, Y, Kondoh, G, Okawa, K, Chujyo, T, Suzuki, T, Hata, K, Kuramochi-Miyagawa, S, Nakano, T, Sasaki, H, Chuma, S, Nakatsuji, N
  • Organizer: Germ Cells, Cold Spring Habor Laboratory Meeting
  • Place of Presentation: Cold spring harbor, USA
  • Year and Date: 20081001-20081005
• [Presentation] Protein and RNA assembly of germinal granules/nuage in the germline. (2008)
  • Author(s): Shinichiro Chuma
  • Organizer: World Congress on Reproductive Biology
  • Place of Presentation: Hawaii, USA
  • Year and Date: 20080524-20080525
• [Presentation] Ultrastructural characteristic of spermatogenesis and its evolutionary conservation in the germline. (2008)
  • Author(s): Shinichiro Chuma
  • Organizer: 15th European testis workshop with NAFA annual meeting
  • Place of Presentation: Naantali, Finland
  • Year and Date: 20080502-20080506