Regulation and function of histone methylation dynamics in germ-lineage cells

2010 Fiscal Year Self-evaluation Report

• Project Area: The germline: its developmental cycle and epigenome network
• Project/Area Number: 20062005
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The Institute of Physical and Chemical Research (2011-2012); Kyoto University (2008-2010)
• Principal Investigator: 眞貝 洋一 Kyoto University, ウイルス研究所, 教授 (20211972)
• Project Period (FY): 2008 – 2012
• Keywords: エピジェネティクス / ヒストン / メチル化 / 脱メチル化 / 生殖細胞

Research Abstract

どのようにしてヒストンのエピジェネティック情報のダイナミズムが制御されているのか、そしてその制御が生命機能にどのようなインパクトを持つのかを解き明かす。そのために、生殖系列細胞の分化・増殖をモデル系として、ヒストンメチル化修飾の役割とその制御の分子基盤を解明する。

Research Products

• [Journal Article] DNA methylation and SETDB1/H3K9me3 regulate predominantly distinct sets of genes, retroelements andchimaeric transcripts in mouse ES cells. (2011)
  • Author(s): Karimi, M.M.Goyal, P.Maksakova, I.A.Bilenky, M.Leung, D.Tang, J.X.Shinkai, Y.Mager, D.L.Jones, S.Hirst M., Lorincz, M.C.
  • Journal Title: Cell Stem Cell. in press
• [Journal Article] H3K9 methyltransferase G9a and the related molecule GLP. (2011)
  • Author(s): Shinkai Y., Tachibana M.
  • Journal Title: Genes Dev. 25 Pages: 781-788
• [Journal Article] Proviral silencing in embryonic stem cells requires the histone methyltransferase ESET. (2010)
  • Author(s): Matsui, T.Leung, D.Miyashita, H.Maksakova, I.A.Miyachi, H.Kimura, H.Tachibana, M.Lorincz, M.C., Shinkai, Y.
  • Journal Title: Nature. 464 Pages: 927-931
• [Journal Article] Histone H1 null vertebrate cells exhibit altered nucleosome architecture. (2010)
  • Author(s): Hashimoto, H.Takami, Y.Sonoda, E.Iwasaki, T.Iwano, H.Tachibana, M.Takeda, S.Nakayama, T.Kimura, H., Shinkai, Y.
  • Journal Title: Nucleic Acids Res. 38 Pages: 3533-3545
• [Journal Article] G9a/GLP complexes independently mediate H3K9 and DNA methylation to silence transcription. (2008)
  • Author(s): Tachibana M.Matsumura Y.Fukuda M.Kimura H., Shinkai Y.
  • Journal Title: EMBO J. 27 Pages: 2681-2690
• [Presentation] Proviral silencing in embryonic stem cells requires the histone methyltransferase ESET. (2010)
  • Author(s): Matsui, T., Leung, D., Miyashita, H., Maksakova, I.A., Miyachi, H., Kimura, H., Tachibana, M., Lorincz, M.C., Shinkai, Y.
  • Organizer: Gordon Research Conference "Chromatn Structure and Function"
  • Place of Presentation: Bryant University, Smithfield, RI, USA
  • Year and Date: 20100725-20100730
• [Presentation] ヒストンリジンメチル化酵素ESET による内在性レトロウイルス抑制機構 (2010)
  • Author(s): 眞貝洋一、松井稔幸
  • Organizer: 日本遺伝学会第82回大会
  • Place of Presentation: 札幌
  • Year and Date: 2010-09-02
• [Presentation] ESET/SETDB1 is essential for LTR-retroelement silencing in mouse embryonic stem cells. (2009)
  • Author(s): Shinkai Y.
  • Organizer: FASEB Summer Research Conferences, "Epigenetics, Chromatin & Transcription".
  • Place of Presentation: Snowmass Village, Colorado, USA
  • Year and Date: 20090712-20090717
• [Presentation] ヒストンリジンメチル化コードと生命機能制御 (2009)
  • Author(s): 眞貝洋一
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-21
• [Presentation] Function and regulation of histone lysine methylation (2008)
  • Author(s): 眞貝洋一
  • Organizer: 第31回日本分子生物学会大会、第81回日本生化学会大会合同大会シンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09