2012 Fiscal Year Final Research Report
Regulation and roles of histone methylation dynamics in germ-lineage cells
| Project Area | The germline: its developmental cycle and epigenome network |
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| Project/Area Number |
20062005
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | The Institute of Physical and Chemical Research (2011-2012)
Kyoto University (2008-2010) |
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Principal Investigator |
SHINKAI Yoichi 独立行政法人理化学研究所, 眞貝細胞記憶研究室, 主任研究員 (20211972)
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| Co-Investigator(Kenkyū-buntansha) |
TACHIBANA Makoto 国立大学法人京都大学, ウイルス研究所・感染症モデル研究センター・ゲノム改変マウス研究領域, 准教授 (80303915)
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| Project Period (FY) |
2008 – 2012
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| Keywords | 生殖細胞 / ヒストン / メチル化 / エピジェネティクス / 内在性転移因子 / 転写制御 |
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| Research Abstract |
1. We discovered that GLP protein expression is posttranscriptionally regulated in murine embryonic male germ cells after sex determination and that low H3K9me2 level results from the absence of GLP (severe reduction of the G9a-GLP heteromeric complex).
2. We demonstrated that that H3K9 methyltransferase G9a/GLP complex suppresses transcription by independently inducing both H3K9 and DNA methylation.
3. We discovered that H3K9 methyltransferase ESET and ESET.mediated H3K9me3 play a crucial role in proviral silencing during the period early in embryogenesis when DNA methylation is dynamically reprogrammed.
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[Journal Article] DNA methylation and SETDB1/H3K9me3 regulate predominantly distinct sets of genes, retroelements and chimaeric transcripts in mouse ES cells.2011
Author(s)
Karimi, M.M. Goyal, P. Maksakova, I.A. Bilenky, M. Leung, D. Tang, J.X. Shinkai, Y. Mager, D.L. Jones, S. Hirst M. and Lorincz, M.C
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Journal Title
Cell Stem Cell
Volume: 8
Pages: 676-687
Peer Reviewed
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[Journal Article] Obesity and metabolic syndrome in histone demethylase JHDM2a deficient mice.2009
Author(s)
Inagaki, T^#. Tachibana, M^#. Magoori, K. Kudo, H. Tanaka, T. Okamura, M. Naito, M. Kodama, T. Shinkai, Y. and Sakai, Y
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Journal Title
Genes to Cells
Volume: 14
Pages: 991-1001
Peer Reviewed
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