Epigenetic Regulation and Small RNA in Spermatogenesis

2010 Fiscal Year Self-evaluation Report

• Project Area: The germline: its developmental cycle and epigenome network
• Project/Area Number: 20062007
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Osaka University
• Principal Investigator: NAKANO Toru Osaka University, 生命機能研究科, 教授 (00172370)
• Project Period (FY): 2008 – 2012
• Keywords: DNAメチル化 / small RNA / 精子形成 / エピジェネティック制御 / 幹細胞

Research Abstract

精子形成において、マウスPIWIファミリーが、どのように生殖細胞特異的なsmall RNAであるpiRNAの産生に関与しているのか、そのpiRNAがどのような機能を有しているのか、さらに、その機能発現機構はどのようなものであるか、を解析することにより、エピジェネティックな遺伝子発現制御にどのように関与しているかを明らかにするものである。

Research Products

• [Journal Article] MVH in piRNA processing and gene silencing of retrotransposons (2010)
  • Author(s): Kuramochi-Miyagawa S, Watanabe T, Gotoh K, Takamatasu K, Chuma S, Kojima-Kita K, Shiromoto Y, Asada N, Toyoda A, Fujiyama A, Totoki Y, Shibata T, Kimura T, Nakatsuji N, Noce T, Sasaki H, Nakano T.
  • Journal Title: Genes Dev 24 Pages: 887-892
  • Peer Reviewed
• [Journal Article] MILI, a piRNA binding protein, is required for germline stem cell self-renewal and appears to positively regulate translation. (2009)
  • Author(s): Unhavaithaya Y, Hao Y, Beyret E, Yin H, Kuramochi-Miyagawa S, Nakano T, Lin H.
  • Journal Title: J Biol Chem 284 Pages: 6507-6519
  • Peer Reviewed
• [Journal Article] The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline (2009)
  • Author(s): Shoji M, Tanaka T, Hosokawa M, Reuter M, Stark A, Kato Y, Kondoh G, Okawa K, Chujo, Suzuki T, Hata K, Martin S, Noce T, Kuramochi-Miyagawa S, Nakano T, Sasaki H, Pillai RS, Nakatsuji N, Chuma S.
  • Journal Title: Dev Cell 17 Pages: 775-787
  • Peer Reviewed
• [Journal Article] DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes. (2008)
  • Author(s): Kuramochi-Miyagawa S, Watanabe T, Gotoh K, Totoki Y, Toyoda A, Ikawa M, Asada N, Kojima K, Yamaguchi Y, Ijiri T, Hata K, Li E, Matsuda Y, Kimura T, Okabe M, Sakaki Y, Sasaki H, Nakano T
  • Journal Title: Genes Dev 22 Pages: 918-930
  • Peer Reviewed
• [Presentation] Regulation of Stem Cell Systems by PI3K/Akt Signaling (2008)
  • Author(s): Nakano T
  • Organizer: The 5th Nikko International Symposium : Stem Cells and Cancer
  • Place of Presentation: Tochigi
  • Year and Date: 2008-10-25