The mechanism of the acquisition of germ cell-specific epigenme and its reconstruction in vitro.

2010 Fiscal Year Self-evaluation Report

• Project Area: The germline: its developmental cycle and epigenome network
• Project/Area Number: 20062011
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University (2009-2012); The Institute of Physical and Chemical Research (2008)
• Principal Investigator: SAITOU Mitinori The Institute of Physical and Chemical Research, 医学研究科, 教授 (80373306)
• Project Period (FY): 2008 – 2012
• Keywords: 発生・分化 / 遺伝子 / ゲノム / シグナル伝達 / 再生医学

Research Abstract

(1)生殖細胞形成機構を試験管内で再構成し、生殖細胞系列が獲得するユニークなエピゲノム情報とその獲得機構の解明、
(2)Prdm14の胚性幹細胞(Embryonic Stem Cells:ESCs)の多能性維持における役割解明、
(3)生殖細胞形成能獲得におけるWnt3及びBmp4シグナルの作用機序解明、を目的とする。

Research Products

• [Journal Article] TDRD5 is required for retrotransposon silencing, chromatoid body assembly and spermiogenesis in mice. (2011)
  • Author(s): Yabuta, Y., Ohta, H., Abe, T., Kurimoro, K., Chuma, S., Saitou, M.
  • Journal Title: The Journal of Cell Biology 192 Pages: 781-795
  • Peer Reviewed
• [Journal Article] Functional reconstruction of Nanos3 expression in the germ cell lineage by a novel transgenic reporter reveals distinct subcellular localizations of Nanos3. (2010)
  • Author(s): Yamaji, M., Tanaka, T., Shigeta, M., Chuma, S., Saga, Y., Saitou, M.
  • Journal Title: Reproduction 139 Pages: 381-393
  • Peer Reviewed
• [Journal Article] A signaling principle for the specification of the germ cell lineage in mice. (2009)
  • Author(s): Ohinata, Y., Ohta, H., Shigeta, M., Yamanaka, K., Wakayama, T., Saitou, M.
  • Journal Title: Cell 137 Pages: 571-584
  • Peer Reviewed
• [Journal Article] Critical function of Prdm14 for the establishment of the germ cell lineage in mice. (2008)
  • Author(s): Yamaji, M., Seki, Y., Kurimoto, K., Yabuta, Y., Yuasa, M., Shigeta, M., Yamanaka, K., Ohinata, Y., Saitou, M.
  • Journal Title: Nature Genetics 40 Pages: 1016-1022
  • Peer Reviewed
• [Journal Article] Complex genome-wide transcription dynamics orchestrated by Blimp1 for the specification of the germ cell lineage in mice. (2008)
  • Author(s): Kurimoto, K., Yabuta, Y., Ohinata, Y., Shigeta, M., Yamanaka, K., Saitou, M.
  • Journal Title: Genes & Development 22 Pages: 1617-1635
  • Peer Reviewed
• [Presentation] Germ cell specification in mice in vivo and in vitro. (2010)
  • Author(s): Saitou, M.
  • Organizer: Cold Spring Harbor Laboratory Meeting on Germ Cells Cold Spring Harbor Laboratory
  • Place of Presentation: New York
  • Year and Date: 2010-10-05
• [Presentation] Germ cell specification in vivo and in vitro. (2010)
  • Author(s): Saitou, M.
  • Organizer: ISSCR 8th Annual Meeting
  • Place of Presentation: San Francisco
  • Year and Date: 2010-06-18
• [Book] A global single-cell cDNA amplification method for quantitative microarray analysis., 687 (2011)
  • Author(s): Kurimoto, K., Saitou, M.
  • Total Pages: 91-111
  • Publisher: Methods in Molecular Biology
• [Remarks] ホームページ
  • URL: http://www.med.kyoto-u.ac.jp/J/grad_school/introduction/1103/
• [Patent(Industrial Property Rights)] METHOD OF INDUCING DIFFERENTIATION FROM PLURIPOTENT STEM CELLS TO GERM CELLS (2010)
  • Inventor(s): 斎藤通紀、林克彦
  • Industrial Property Rights Holder: 国立大学法人京都大学
  • Industrial Property Number: 特許,61/373, 563
  • Filing Date: 2010-08-13