Nuclear transfer study for the mechanisms of acquisition of totipotency by the germ cell genome

2010 Fiscal Year Self-evaluation Report

• Project Area: The germline: its developmental cycle and epigenome network
• Project/Area Number: 20062012
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: OGURA Atsuo The Institute of Physical and Chemical Research, 遺伝工学基盤技術室, 室長 (20194524)
• Project Period (FY): 2008 – 2012
• Keywords: 核移植 / 生殖細胞 / 胚盤胞 / 遺伝子発現 / マウス / 再プログラム化

Research Abstract

本計画研究は、核移植クローン技術と分子生物学的解析技術を組み合わせることにより、生殖細胞特有の全能性獲得に関わるゲノム構造あるいは機能を明らかにすることを目的とする。本研究では、全能性獲得の機構(あるいはその準備)こそが次世代にゲノムを伝える生殖細胞の本質であるとの認識をもとに、それがいつどのように生じるか、そしてその際にゲノム上にどのようなエピジェネティクス変化が起こっているかを明らかにする。生殖細胞ゲノムが正常にリプログラミングされるためのいくつかの条件が明らかにされ、さらにそれを体細胞クローンの改善により証明できた。最終的には、体細胞ゲノムの初期化の人為的制御を目指したい。

Research Products

• [Journal Article] Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer. (2010)
  • Author(s): Inoue, K., Kohda, T., (14名省略), Ogura, A.
  • Journal Title: Science 330 Pages: 496-499
  • Peer Reviewed
• [Journal Article] The effect on intracytoplasmic sperm injection outcome of genotype, male germ cell stage and freeze-thawing in mice. (2010)
  • Author(s): Ogonuki N., 6名省略, Ogura, A.
  • Journal Title: PLoS ONE 5 Pages: e11062
  • Peer Reviewed
• [Journal Article] Large-scale production of growing oocytes in vitro from neonatal mouse ovaries. (2009)
  • Author(s): Honda, A., 10名省略, Ogura, A.
  • Journal Title: Int.J.Dev.Biol. 53 Pages: 605-613
  • Peer Reviewed
• [Journal Article] Sex-reversed somatic cell cloning in the mouse. (2009)
  • Author(s): Inoue, K., Ogonuki, N., Mekada, K., Yoshiki, A., Sado, T., Ogura, A.
  • Journal Title: J.Reprod.Dev. 55 Pages: 566-569
  • Peer Reviewed
• [Journal Article] Generation of induced pluripotent stem cells in rabbits: potential experimental models for human regenerative medicine.
  • Author(s): Honda, A., 5名省略 , Ogura, A.
  • Journal Title: J.Biol.Chem. in press
  • Peer Reviewed
• [Presentation] Recent technical advancements of nuclear transfer in the mouse. (2010)
  • Author(s): Ogura A.
  • Organizer: 4th AFLAS Congress Meeting/5th AMMRA Annual Meeting/11th CSLAS Annual Meeting
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 20101100
• [Presentation] Improvement of somatic cell nuclear transfer in mice : Genetic and epigenetic approaches. (2010)
  • Author(s): Ogura A, Inoue K
  • Organizer: Czeck-Japan Joint Symposium for Animal Reproduction, "From Gametes to Stem Cells
  • Place of Presentation: Liblice Castle, Czech Republic
  • Year and Date: 2010-09-21
• [Presentation] 雄性生殖細胞の受精能とゲノム刷り込み (2009)
  • Author(s): 小倉淳郎
  • Organizer: 農業生物資源研究所 生殖機構研究シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 20091200
• [Presentation] Micro-insemination and nuclear transfer using male germ cells. (2008)
  • Author(s): Ogura A.
  • Organizer: World Congress on Reproductive Biology
  • Place of Presentation: Hawaii, U.S.A.
  • Year and Date: 2008-05-24
• [Book] 遺伝子の導入法とノックアウト法(生命の誕生に向けて) (2011)
  • Author(s): 小倉淳郎、井上貴美子
  • Total Pages: 273-276
  • Publisher: 近代出版
• [Remarks] 「クローンマウス出生率10倍に」(日経産業新聞20100917・研究室のホームページを開設・更新した
  • URL: http://www.brc.riken.go.jp/lab/kougaku/