2012 Fiscal Year Final Research Report
Nuclear transfer for the analysis of the mechanisms of totipotency acquisition in the germline
| Project Area | The germline: its developmental cycle and epigenome network |
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| Project/Area Number |
20062012
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
OGURA Atsuo 独立行政法人理化学研究所, 遺伝工学基盤技術室, 室長 (20194524)
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| Co-Investigator(Kenkyū-buntansha) |
KOHDA Takashi 東京医科歯科大学, 難治疾患研究所, 准教授 (60211893)
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| Co-Investigator(Renkei-kenkyūsha) |
ISHINO Fumitoshi 東京医科歯科大学, 難治疾患研究所, 教授 (60159754)
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| Project Period (FY) |
2008 – 2012
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| Keywords | 核移植 / 全能性 / 生殖系列 / エピジェネティクス |
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| Research Abstract |
The germ cell genome can acquire totipotency, the genomic state of early fertilized embryos. To obtain clues to understanding the mechanisms of acquisition of totipotency by the germ cells, the epigenetic characteristics in the embryos reconstructed with different donor cell nuclei during the life cycle were analyzed using the mouse as the model. The results indicated that mature MII oocytes could reprogram all the epigenetic modifications with only some exceptions including a certain histone tail methylation.
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[Journal Article] RNAi-mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryos.2011
Author(s)
Matoba S, Inoue K, Kohda T, Sugimioto M, Mizutani E, Ogonuki N, Nakamura T, Abe K, Nakano T, Ishino F, and Ogura A
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Journal Title
Proc Natl Acad Sci USA
Volume: 108
Pages: 20621-20626
Peer Reviewed
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[Journal Article] Intracytoplasmic sperm injection induces transcriptome perturbation without any transgenerational effect.2011
Author(s)
Kohda T, Ogonuki N, Inoue K, Furuse T, Kaneda H, Suzuki T, Kaneko-Ishino T, Wakayama T, Wakana S, Ogura A, Ishino F
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Journal Title
Biochem Biophys Res Commun
Volume: 410
Pages: 282-288
Peer Reviewed
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[Journal Article] Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer.2010
Author(s)
Inoue K, Kohda T, Sugimoto M, Sado T, Ogonuki N, Matoba S, Shiura H, Ikeda R, Mochida K, Fujii T, Sawai K, Otte AP, Tian XC, Yang X, Ishino F, Abe K, Ogura A
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Journal Title
Science
Volume: 330
Pages: 496-499
Peer Reviewed
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[Journal Article] Efficient production of androgenetic embryos by round spermatid injection.2009
Author(s)
Miki H, Hirose M, Ogonuki N, Inoue K, Kezuka F, Honda A, Mekada K, Hanaki K, Iwafune H, Yoshiki A, Ishino F, Ogura A
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Journal Title
Genesis
Volume: 47
Pages: 155-60
Peer Reviewed
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[Journal Article] Large-scale production of growing oocytes in vitro from neonatal mouse ovaries.2009
Author(s)
Honda A, Hirose M, Inoue K, Hiura H, Miki H, Ogonuki N, Sugimoto M, Abe K, Kanatsu-Shinohara M, Kono T, Shinohara T, Ogura A
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Journal Title
Int J Dev Biol
Volume: 53
Pages: 605-613
Peer Reviewed
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[Journal Article] Stable ES cell lines in rabbits - potential small animal models for human ES cell research.2008
Author(s)
Honda A, Hirose M, Inoue K, Ogonuki N, Miki H, Shimozawa N, Hatori M, Shimizu N, Murata T, Hirose H, Katayama K, Wakisaka N, Miyoshi H, Yokoyama KK, Sankai T, Ogura A
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Journal Title
Reprod Biomed Online
Volume: 17
Pages: 706-715
Peer Reviewed
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[Journal Article] Role of retrotransposon-derived imprinted gene, Rtl1, in the feto-maternal interface of mouse placenta.2008
Author(s)
Sekita Y, Wagatsuma H, Nakamura K, Ono R, Kagami M, Wakisaka N, Hino T, Suzuki-Migishima R, Kohda T, Ogura A, Ogata T, Yokoyama M, Kaneko-Ishino T, Ishino F
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Journal Title
Nat Genet
Volume: 40
Pages: 243-248
Peer Reviewed
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[Journal Article] RNAi-mediated knockdown of Xist does not rescue the impaired development of female cloned mouse embryos.
Author(s)
Oikawa M, Matoba S, Inoue K, Kamimura S, Hirose M, Ogonuki N, Shiura H, Sugimito M, Abe K, Ishino F, Ogura A
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Journal Title
J Reprod Dev
Volume: (in press)
Peer Reviewed
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[Journal Article] A high-speed congenic strategy using first-wave male germ cells.
Author(s)
Ogonuki N, Inoue K, Hirose M, Miura I, Mochida K, Sato T, Mise N, Mekada K, Yoshiki A, Abe K, Kurihara H, Wakana S, Ogura A
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Journal Title
PLoS One
Volume: 4(3)
Pages: e4943
DOI
Peer Reviewed
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