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2012 Fiscal Year Final Research Report

Nuclear transfer for the analysis of the mechanisms of totipotency acquisition in the germline

Planned Research

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Project AreaThe germline: its developmental cycle and epigenome network
Project/Area Number 20062012
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

OGURA Atsuo  独立行政法人理化学研究所, 遺伝工学基盤技術室, 室長 (20194524)

Co-Investigator(Kenkyū-buntansha) KOHDA Takashi  東京医科歯科大学, 難治疾患研究所, 准教授 (60211893)
Co-Investigator(Renkei-kenkyūsha) ISHINO Fumitoshi  東京医科歯科大学, 難治疾患研究所, 教授 (60159754)
Project Period (FY) 2008 – 2012
Keywords核移植 / 全能性 / 生殖系列 / エピジェネティクス
Research Abstract

The germ cell genome can acquire totipotency, the genomic state of early fertilized embryos. To obtain clues to understanding the mechanisms of acquisition of totipotency by the germ cells, the epigenetic characteristics in the embryos reconstructed with different donor cell nuclei during the life cycle were analyzed using the mouse as the model. The results indicated that mature MII oocytes could reprogram all the epigenetic modifications with only some exceptions including a certain histone tail methylation.

  • Research Products

    (41 results)

All 2012 2011 2010 2009 2008 Other

All Journal Article (23 results) (of which Peer Reviewed: 23 results) Presentation (7 results) Book (9 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] PGC7 binds histone H3K9me2 to protect against conversion of 5mC to 5hmC in early embryos.2012

    • Author(s)
      Nakamura T, Liu Y-J, Nakashima H, Umehara H, Inoue K, Matoba S, Tachibana M, Ogura A, Shinkai Y, Nakano T
    • Journal Title

      Nature

      Volume: 486 Pages: 415-419

    • Peer Reviewed
  • [Journal Article] Efficient production of offspring from Japanese wild-derived strains of mice (Mus musculus molossinus) by improved assisted reproductive technologies.2012

    • Author(s)
      Hasegawa A, Mochida K, Matoba S, Yonezawa K, Ohta A, Watanabe G, Taya K, Ogura A
    • Journal Title

      Biol Reprod

      Volume: 86: 86: 167 Pages: 1-7

    • Peer Reviewed
  • [Journal Article] Optimization of a protocol for cryopreservation of mouse spermatozoa using cryotubes.2012

    • Author(s)
      Hasegawa A, Yonezawa K, Ohta A, Mochida K, Ogura A
    • Journal Title

      J Reprod Dev

      Volume: 58 Pages: 156-161

    • Peer Reviewed
  • [Journal Article] Recent advancements in cloning by somatic cell nuclear transfer.2011

    • Author(s)
      Ogura A, Inoue K, Wakayama T
    • Journal Title

      Philos Trans RSoc Lond B Biol Sci

      Volume: 368 Pages: 0329

    • URL

      http://dx.doi.org/10.1098/rstb.2011.0329

    • Peer Reviewed
  • [Journal Article] RNAi-mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryos.2011

    • Author(s)
      Matoba S, Inoue K, Kohda T, Sugimioto M, Mizutani E, Ogonuki N, Nakamura T, Abe K, Nakano T, Ishino F, and Ogura A
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 108 Pages: 20621-20626

    • Peer Reviewed
  • [Journal Article] Intracytoplasmic sperm injection induces transcriptome perturbation without any transgenerational effect.2011

    • Author(s)
      Kohda T, Ogonuki N, Inoue K, Furuse T, Kaneda H, Suzuki T, Kaneko-Ishino T, Wakayama T, Wakana S, Ogura A, Ishino F
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 410 Pages: 282-288

    • Peer Reviewed
  • [Journal Article] In vitro production of functional sperm in cultured neonatal mouse testes.2011

    • Author(s)
      Sato T, Katagiri K, Gohbara A, Inoue K, Ogonuki N, Ogura A, Kubota Y, & Ogawa T
    • Journal Title

      Nature

      Volume: 471 Pages: 504-507

    • Peer Reviewed
  • [Journal Article] Birth of normal mice following round spermatid injection without artificial oocyte activation.2011

    • Author(s)
      Ogonuki N, Inoue K, Ogura A
    • Journal Title

      J Reprod Dev

      Volume: 57 Pages: 534-538

    • Peer Reviewed
  • [Journal Article] Generation of functional oocytes and spermatids from fetal primordial germ cells after ectopic transplantation in adult animals.2011

    • Author(s)
      Matoba S, Ogura A
    • Journal Title

      Biol Reprod

      Volume: 84 Pages: 631-838

    • Peer Reviewed
  • [Journal Article] Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer.2010

    • Author(s)
      Inoue K, Kohda T, Sugimoto M, Sado T, Ogonuki N, Matoba S, Shiura H, Ikeda R, Mochida K, Fujii T, Sawai K, Otte AP, Tian XC, Yang X, Ishino F, Abe K, Ogura A
    • Journal Title

      Science

      Volume: 330 Pages: 496-499

    • Peer Reviewed
  • [Journal Article] Generation of induced pluripotent stem cells in rabbits: potential experimental models for human regenerative medicine.2010

    • Author(s)
      Honda A, Hirose M, Hatori M, Matoba S, Miyoshi H, Inoue K, Ogura A
    • Journal Title

      J Biol Chem

      Volume: 285 Pages: 31362-31369

    • Peer Reviewed
  • [Journal Article] Sex-reversed somatic cell cloning in the mouse.2009

    • Author(s)
      Inoue K, Ogonuki N, Mekada K, Yoshiki A, Sado T, Ogura A
    • Journal Title

      Reprod Dev

      Volume: 55 Pages: 566-569

    • Peer Reviewed
  • [Journal Article] Embryonic rather than extraembryonic tissues have more impact on the development of placental hyperplasia in cloned mice.2009

    • Author(s)
      Miki H, Wakisaka N, Inoue K, Ogonuki N, Mori M, Kim JM, Ohta A, Ogura A
    • Journal Title

      Placenta

      Volume: 30 Pages: 543-546

    • Peer Reviewed
  • [Journal Article] Changes in allele-specific association of histone modifications at the imprinting control regions during mouse preimplantation development.2009

    • Author(s)
      Kim J-M, Ogura A
    • Journal Title

      Genesis

      Volume: 47 Pages: 611-616

    • Peer Reviewed
  • [Journal Article] Basic FGF and Activin/Nodal but not LIF signaling sustain undifferentiated status of rabbit embryonic stem cells.2009

    • Author(s)
      Honda A, Hirose M, Ogura A
    • Journal Title

      Exp Cell Res

      Volume: 315 Pages: 2033-2042

    • Peer Reviewed
  • [Journal Article] Efficient production of androgenetic embryos by round spermatid injection.2009

    • Author(s)
      Miki H, Hirose M, Ogonuki N, Inoue K, Kezuka F, Honda A, Mekada K, Hanaki K, Iwafune H, Yoshiki A, Ishino F, Ogura A
    • Journal Title

      Genesis

      Volume: 47 Pages: 155-60

    • Peer Reviewed
  • [Journal Article] Large-scale production of growing oocytes in vitro from neonatal mouse ovaries.2009

    • Author(s)
      Honda A, Hirose M, Inoue K, Hiura H, Miki H, Ogonuki N, Sugimoto M, Abe K, Kanatsu-Shinohara M, Kono T, Shinohara T, Ogura A
    • Journal Title

      Int J Dev Biol

      Volume: 53 Pages: 605-613

    • Peer Reviewed
  • [Journal Article] Stable ES cell lines in rabbits - potential small animal models for human ES cell research.2008

    • Author(s)
      Honda A, Hirose M, Inoue K, Ogonuki N, Miki H, Shimozawa N, Hatori M, Shimizu N, Murata T, Hirose H, Katayama K, Wakisaka N, Miyoshi H, Yokoyama KK, Sankai T, Ogura A
    • Journal Title

      Reprod Biomed Online

      Volume: 17 Pages: 706-715

    • Peer Reviewed
  • [Journal Article] Ultrastructure of placental hyperplasia in mice: Comparison of placental phenotypes with three different etiologies.2008

    • Author(s)
      Wakisaka N, Inoue K, Ogonuki N, Miki H, Sekita Y, Hanaki K, Akatsuka K, Kaneko-Ishino T, Ishino F, Ogura A
    • Journal Title

      Placenta

      Volume: 29 Pages: 753-759

    • Peer Reviewed
  • [Journal Article] Role of retrotransposon-derived imprinted gene, Rtl1, in the feto-maternal interface of mouse placenta.2008

    • Author(s)
      Sekita Y, Wagatsuma H, Nakamura K, Ono R, Kagami M, Wakisaka N, Hino T, Suzuki-Migishima R, Kohda T, Ogura A, Ogata T, Yokoyama M, Kaneko-Ishino T, Ishino F
    • Journal Title

      Nat Genet

      Volume: 40 Pages: 243-248

    • Peer Reviewed
  • [Journal Article] RNAi-mediated knockdown of Xist does not rescue the impaired development of female cloned mouse embryos.

    • Author(s)
      Oikawa M, Matoba S, Inoue K, Kamimura S, Hirose M, Ogonuki N, Shiura H, Sugimito M, Abe K, Ishino F, Ogura A
    • Journal Title

      J Reprod Dev

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] The effect on intracytoplasmic sperm injection outcome of genotype, male germ cell stage and freeze-thawing in mice.

    • Author(s)
      Ogonuki N, Mori1 M, Shinmen A, Inoue K, Mochida K, Ohta A, Ogura A
    • Journal Title

      PLoS ONE

      Volume: 5(6) Pages: e11062

    • DOI

      doi:10.1371/journal.pone.0011062

    • Peer Reviewed
  • [Journal Article] A high-speed congenic strategy using first-wave male germ cells.

    • Author(s)
      Ogonuki N, Inoue K, Hirose M, Miura I, Mochida K, Sato T, Mise N, Mekada K, Yoshiki A, Abe K, Kurihara H, Wakana S, Ogura A
    • Journal Title

      PLoS One

      Volume: 4(3) Pages: e4943

    • DOI

      doi:10.1371/journal.pone.0004943

    • Peer Reviewed
  • [Presentation] Analysis of the dynamics of imprinted X chromosome inactivation in mice by nuclear transfer2012

    • Author(s)
      Ogura A, Oikawa M, Matoba S, Inoue K
    • Organizer
      The 9th Annual Conference of the Asian Reproductive Biotechnology Society Manila Philippine
    • Place of Presentation
      Manila Philippine
    • Year and Date
      2012-10-24
  • [Presentation] Nuclear transfer for the study of X chromosome inactivation in mice2012

    • Author(s)
      Ogura A, Oikawa M, Inoue K
    • Organizer
      Mouse Molecular Genetics California USA
    • Place of Presentation
      California USA
    • Year and Date
      2012-10-04
  • [Presentation] Nuclear transfer for the study of epigenetic dynamics in mammalian development2012

    • Author(s)
      Ogura A
    • Organizer
      The 84th Annual Meeting of the Genetics Society of Japan Fukuoka
    • Place of Presentation
      Fukuoka
    • Year and Date
      2012-09-24
  • [Presentation] Development of ES/iPS cell research using rabbits2012

    • Author(s)
      Honda A, Hirose M, Hatori M, Ogura A
    • Organizer
      SRD-NIAS the 2nd Japan-Czech Joint Symposium Tokyo
    • Place of Presentation
      Tokyo
    • Year and Date
      2012-09-10
  • [Presentation] X-chromosome inactivation status in cloned mouse embryos Epigenetic Memory2012

    • Author(s)
      Ogura A
    • Organizer
      The Company of Biologists Workshop Steyning UK
    • Place of Presentation
      Steyning UK
    • Year and Date
      2012-06-26
  • [Presentation] Transportation of frozen and unfrozen materials2012

    • Author(s)
      Mochida K, Ogura A
    • Organizer
      EMMA cryopreservation workshop Spain Madrid
    • Place of Presentation
      Spain Madrid,
    • Year and Date
      2012-05-08
  • [Presentation] ICSI and its practical applications2012

    • Author(s)
      Ogura A
    • Organizer
      EMMA Cryopreservation Workshop Madrid Spain
    • Place of Presentation
      Madrid Spain
    • Year and Date
      2012-05-08
  • [Book] 「核移植技術:Xist の機能抑制による体細胞クローン作出効率の改善法」細胞工学2012

    • Author(s)
      的場章悟,井上貴美子,小倉淳郎
    • Total Pages
      282-287
  • [Book] 「核移植クローン技術の未来は?」細胞工学2012

    • Author(s)
      小倉淳郎
    • Total Pages
      288-289
  • [Book] 細胞培養プロトコール(中村幸夫編)2012

    • Author(s)
      廣瀬美智子,小倉淳郎
    • Total Pages
      187-197
    • Publisher
      羊土社
  • [Book] 遺伝子の導入法とノックアウト法 生命の誕生に向けて(第二版)2011

    • Author(s)
      小倉 淳郎, 井上 貴美子
    • Total Pages
      273-276
    • Publisher
      近代出版 東京
  • [Book] 顕微授精と体外受精の比較論卵子学 (総編集者 森 崇英)2011

    • Author(s)
      小倉 淳郎
    • Total Pages
      757-766
    • Publisher
      京都大学学術出版会 京都
  • [Book] 活性X 染色体からのXist 遺伝子の発現の抑制はマウス体細胞クローンの効率を改善するライフサイエンス新着論文レビュー Vol.11902010

    • Author(s)
      井上 貴美子, 小倉 淳郎
    • Total Pages
      1-6
  • [Book] 新生仔マウス卵巣から分離された莢膜幹細胞と卵子の特徴について 日本生殖内分泌学会雑誌 Vol.152010

    • Author(s)
      本多 新, 小倉 淳郎
    • Total Pages
      19-24
  • [Book] 核移植によるリプログラミング 細胞 Vol.42 122010

    • Author(s)
      小倉 淳郎, 井上 貴美子
    • Total Pages
      489-493
  • [Book] 体細胞クローン動物のエピジェネティクスと表現型 日本胚移植学雑誌Vol.31 22009

    • Author(s)
      小倉 淳郎
    • Total Pages
      87-93
  • [Remarks]

    • URL

      http://www.brc.riken.go.jp/lab/kougaku/

  • [Patent(Industrial Property Rights)] クローン動物の作出方法2010

    • Inventor(s)
      小倉淳郎,井上貴美子,石野史敏,幸田尚,澤井 健
    • Industrial Property Rights Holder
      小倉淳郎,井上貴美子,石野史敏,幸田尚,澤井 健
    • Industrial Property Number
      2010-197583
    • Filing Date
      2010-09-03

URL: 

Published: 2014-08-29  

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