Study of nuclear reprogramming using mammalian oocyte

2010 Fiscal Year Self-evaluation Report

• Project Area: The germline: its developmental cycle and epigenome network
• Project/Area Number: 20062015
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: University of Yamanashi (2012); The Institute of Physical and Chemical Research (2008-2011)
• Principal Investigator: WAKAYAMA Teruhiko The Institute of Physical and Chemical Research, ゲノム・リプログラミング研究チーム, チームリーダー (40360672)
• Project Period (FY): 2008 – 2012
• Keywords: 初期化 / リプログラミング / 核移植 / クローン動物 / 卵子

Research Abstract

体細胞クローン動物の成功率は現在でも数%しかなく、エピゲノムによる異常がほぼすべてのクローン動物に生じてしまう。その原因は卵子による初期化が不完全であるためだと考えられているが、初期化機構そのものがまったくわかっていない。そこで本研究では、核移植技術を様々な条件で行い、DNA及びヒストンの修飾変化、卵子細胞質の効果、インプリント遺伝子の発現変化、およびマウスの遺伝的バックグランドの影響などについて検討する。最終的に卵子による核の初期化機構の解明を目指すと同時に初期化の促進方法を開発し、体細胞核の完全初期化および異常が生じない健全なクローン個体を高率に作出することを目指している。

Research Products

• [Journal Article] Functional full-term placentas formed from parthenogenetic embryos using serial nuclear transfer (2010)
  • Author(s): Hikichi T., Ohta H., Wakayama S., Wakayama T.
  • Journal Title: Development 137 Pages: 2841-2847
  • Peer Reviewed
• [Journal Article] Inhibition of Class IIb Histone Deacetylase Significantly Improves Cloning Efficiency in Mice (2010)
  • Author(s): Ono T., Li C., Mizutani E., Terashita Y., Yamagata K., Wakayama T.
  • Journal Title: Biol Reprod 83 Pages: 929-937
  • Peer Reviewed
• [Journal Article] The cytoplasm of mouse germinal vesicle (GV) stage oocytes can enhance somatic cell nuclear reprogramming (2008)
  • Author(s): Bui HT., Wakayama S., Kishigami S., Thuan NV.Wakayama T.
  • Journal Title: Development 135 Pages: 3935-3945
  • Peer Reviewed
• [Journal Article] Production of healthy cloned mice from bodies frozen at -20^0C for 16 years (2008)
  • Author(s): Wakayama S, Ohta H, Hikichi T, Mizutani E, Iwaki T, Kanagawa O, Wakayama T
  • Journal Title: Proc Natl Acad Sci U S A. 105 Pages: 17318-17322
  • Peer Reviewed
• [Presentation] Nuclear transfer as a new tool for preserving mouse strain. (2010)
  • Author(s): Wakayama T.
  • Organizer: 9th Transgenic Technology meeting
  • Place of Presentation: Berlin Germany
  • Year and Date: 20100322-20100324
• [Presentation] Nuclear transfer as a new tool for study of reproduction and genome preservation.Epigenetics, chromatin & Transcription (2010)
  • Author(s): Wakayama T.
  • Organizer: Cold Spring Harbor Conferences Asia
  • Place of Presentation: Suzhou, China
  • Year and Date: 20100317-20100320
• [Presentation] Nuclear transfer ES cells as a new tool for basic biology. (2009)
  • Author(s): Wakayama T.
  • Organizer: The sixth Annual Meeting of Asian Reproductive Biotechnology Society.
  • Place of Presentation: Siem Reap City, Cambodia
  • Year and Date: 20091116-20091118
• [Presentation] Improvement of efficiency of mouse cloning and ES cell establishment. (2008)
  • Author(s): Wakayama T.
  • Organizer: 12th ADNAT convention.The Biology of Embryonic and Adult Stem Cells, Centre for Cellular and Molecular Biology
  • Place of Presentation: Hyderabad, INDIA
  • Year and Date: 20080223-20080224
• [Book] From Molecular Embryologyto Tissue Engineering(ed.Krishnarao Appasani Raghu K.Appasani)(USA.Stem Cells & Regenerative Medicine) (2010)
  • Author(s): Wakayama S.Mizutani E., Wakayama T.
  • Total Pages: 351-369
  • Publisher: Humana Press
• [Book] Methods in Molecular Biology(ed.Ralf Kuehn, Wolfgang Wurst)(USA.Gene Knockout Protocols)530 (2009)
  • Author(s): Wakayama S, Kishigami S, Wakayama T.
  • Total Pages: 251-265
  • Publisher: Humana Press
• [Book] マンモスへの道-クローン技術最前線の技術 における発生・再生医療技術を探る(クローン) (2009)
  • Author(s): 若山照彦
  • Total Pages: 80
  • Publisher: アドスリー社 東京
• [Book] Inc.New York USA, Stem Cell Applications in Diseases(ed.Mikkel L.Sorensen) (2008)
  • Author(s): Wakayama S, Wakayama T.
  • Total Pages: 189-214
  • Publisher: Nova Science Publishers
• [Remarks] ホームページ
  • URL: http://www.cdb.riken.go.jp/grp/
• [Patent(Industrial Property Rights)] 動物組織からクローン動物ならびにntES 細胞を作成するための新規方法 (2009)
  • Inventor(s): 若山清香, 若山照彦
  • Industrial Property Rights Holder: 若山清香, 若山照彦
  • Industrial Property Number: 特許,特願2008-123133
  • Filing Date: 2009-11-19