How to enhance the nuclear reprogramming by oocyte cytoplasm and analysis the mechanisum

2012 Fiscal Year Final Research Report

• Project Area: The germline: its developmental cycle and epigenome network
• Project/Area Number: 20062015
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: University of Yamanashi (2012); The Institute of Physical and Chemical Research (2008-2011)
• Principal Investigator: WAKAYAMA Teruhiko 山梨大学, 大学院・医学工学総合研究部, 教授 (40360672)
• Project Period (FY): 2008 – 2012
• Keywords: 応用動物 / 再生医学 / 獣医学 / 畜産学 / 発生分化

Research Abstract

We could demonstrate several possibilities. 1. The cloning success rate was improved up to 10% by HDACis. 2. Health cloned mice were born from frozen dead mouse, which preserved 16 years under -20C. 3. Over six hundreds cloned mice were born from one donor mouse by repeating nuclear transfer up to 27 times.

Research Products

• [Journal Article] Nicotinamide: a Class III HDACi Delays In Vitro Aging of Mouse Oocytes (2013)
  • Author(s): Lee AR, Kishigami S, Amano T, Matsumoto K, Wakayama T, Hosoi Y
  • Journal Title: J Reprod Dev Volume: (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Successful Serial Recloning in the Mouse over Multiple Generations (2013)
  • Author(s): Wakayama S, Kohda T, Obokata H, Tokoro M, Li C, Terashita Y, Mizutani E, Nguyen VT, Kishigami S, Ishino F, Wakayama T
  • Journal Title: Cell Stem Cell Volume: 12(3) (Cover article of issue) Pages: 293-7
  • Peer Reviewed
• [Journal Article] Nuclear transferred embryonic stem cell for analysis of B1 B-lymphocyte development (2012)
  • Author(s): Takase M, Iida R, Maruya M, Sakaue-Sawano A, Miyawaki A, Wakayama T, Nishigami S, Fagarasan S, Kanagawa O
  • Journal Title: Int Immunol. Int Immunol Volume: 25(3) Pages: 145-156
  • Peer Reviewed
• [Journal Article] Gene Expression Profile Normalization in Cloned Mice by Trichostatin A Treatment (2012)
  • Author(s): Kohda T, Kishigami S, Kaneko-Ishino T, Wakayama T, Ishino F
  • Journal Title: Cell Reprogram Volume: 14 Pages: 45-55
  • Peer Reviewed
• [Journal Article] Abnormal chromosome segregation at early cleavage is a major cause of the full-term developmental failure of mouse clones (2012)
  • Author(s): Mizutani E, Yamagata K, Ono T, Akagi S, Geshi M, Wakayama T
  • Journal Title: Dev Biol. 2012 Volume: 364 Pages: 56-65
  • Peer Reviewed
• [Journal Article] Fluorescence cell imaging and manipulation using conventional halogen lamp microscopy (2012)
  • Author(s): Yamagata K, Iwamoto D, Terashita Y, Li C, Wakayama S, Hayashi-Takanaka Y, Kimura H, Saeki K, Wakayama T
  • Journal Title: PLoS One Volume: 7(2) Pages: e31638
  • Peer Reviewed
• [Journal Article] Latrunculin A Can Improve the Birth Rate of Cloned Mice and Simplify the Nuclear Transfer Protocol by Gently Inhibiting Actin Polymerization (2012)
  • Author(s): Terashita Y, Wakayama S, Yamagata K, Li C, Sato E, Wakayama T
  • Journal Title: Biol Reprod Volume: 86 Pages: 180 1-6
  • Peer Reviewed
• [Journal Article] Offspring from mouse embryos developed using a simple incubator-free culture system with a deoxidizing agent (2012)
  • Author(s): Itoi F, Tokoro M, Terashita Y, Yamagata K, Fukunaga N, Asada Y, Wakayama T
  • Journal Title: PLoS One Volume: 7 Pages: e47512
  • Peer Reviewed
• [Journal Article] Essential role of paternal chromatin in the regulation of transcriptional activity during mouse preimplantation development (2011)
  • Author(s): Bui HT, Wakayama S, Mizutani E, Park KK, Kim JH, Van Thuan N, Wakayama T
  • Journal Title: Reproduction Volume: 141 Pages: 67-77
  • Peer Reviewed
• [Journal Article] Choice of random rather than imprinted X inactivation in female embryonic stem cell-derived extra-embryonic cells (2011)
  • Author(s): Murakami K, Araki K, Ohtsuka S, Wakayama T, Niwa H
  • Journal Title: Development Volume: 138 Pages: 197-202
  • Peer Reviewed
• [Journal Article] Generation of Healthy Cloned Mice using Enucleated Cryopreserved Oocytes (2011)
  • Author(s): Hirata S, Fukasawa H, Wakayama S, Wakayama T, Hoshi K
  • Journal Title: Cellular Reprogramming Volume: 13 Pages: 7-11
  • Peer Reviewed
• [Journal Article] Analysis of proteomic profiling of mouse embryonic stem cells derived from fertilized, parthenogenetic and androgenetic blastocysts (2011)
  • Author(s): Cui, XS., Shen, XH., Lee CK., Kang, YK., Wakayama, T. Kim NH
  • Journal Title: Stem Cell Discovery Volume: 1 Pages: 1-15
  • Peer Reviewed
• [Journal Article] Effect of Fluorescent Mercury Light Irradiation on In Vitro and In Vivo Development of Mouse Oocytes after Parthenogenetic Activation or Sperm Microinjection (2011)
  • Author(s): Terashita Y, Li C, Yamagata K, Sato E, Wakayama T
  • Journal Title: J Reprod Dev Volume: 57(Cover article of issue) Pages: 564-571
  • Peer Reviewed
• [Journal Article] Intracytoplasmic sperm injection induces transcriptome perturbation without any transgenerational effect (2011)
  • Author(s): Kohda T, Ogonuki N, Inoue K, Furuse T, Kaneda H, Suzuki T, Kaneko-Ishino T, Wakayama T, Wakana S, Ogura A, Ishino F
  • Journal Title: Biochem Biophys Res Commun Volume: 410 Pages: 282-288
  • Peer Reviewed
• [Journal Article] Intracytoplasmic Sperm Injection with Mouse Spermatozoa Preserved Without Freezing for Six Months Can Lead to Full-Term Development (2011)
  • Author(s): Li C, Mizutani E, Ono T, Terashita Y, Jia XF, Shi HJ, Wakayama T
  • Journal Title: Biol Reprod Volume: 85 Pages: 1183-1190
  • Peer Reviewed
• [Journal Article] Histone Deacetylase Inhibition Improves Activation of Ribosomal RNA Genes and Embryonic Nucleolar Reprogramming in Cloned Mouse Embryos (2011)
  • Author(s): Bui HT, Seo HJ, Park MR, Park JY, Van Thuan N, Wakayama T, Kim JH
  • Journal Title: Biol Reprod Volume: 85 Pages: 1048-1056
  • Peer Reviewed
• [Journal Article] A role for the elongator complex in zygotic paternal genome demethylation (2010)
  • Author(s): Okada, Y. Yamagata, K. Hong, K. Wakayama, T. Zhang, Y
  • Journal Title: Nature Volume: 463 Pages: 554-558
  • Peer Reviewed
• [Journal Article] Effect of Trichostatin A on chromatin remodeling, histone modifications, DNA replication and transcriptional activity in cloned mouse embryos (2010)
  • Author(s): Bui H.T., Wakayama S., Kishigami S., Kim HJ., Van Thuan N., and Wakayama T
  • Journal Title: Biol Reprod Volume: 83 Pages: 454-463
  • Peer Reviewed
• [Journal Article] DNA Methylation Is Dispensable for the Growth and Survival of the Extraembryonic Lineages (2010)
  • Author(s): Sakaue M, Ohta H, Kumaki Y, Oda M, Sakaide Y, Matsuoka C, Yamagiwa A, Niwa H, Wakayama T, Okano M
  • Journal Title: Curr Biol Volume: 20 Pages: 1452-1457
  • Peer Reviewed
• [Journal Article] Functional full-term placentas formed from parthenogenetic embryos using serial nuclear transfer (2010)
  • Author(s): Hikichi T., Ohta H., Wakayama S., and Wakayama T
  • Journal Title: Development Volume: 137 Pages: 2841-2847
  • Peer Reviewed
• [Journal Article] Survival and death of epiblast cells during embryonic stem cell derivation revealed by long-term live-cell imaging with an Oct4 reporter system (2010)
  • Author(s): Yamagata K, Ueda J, Mizutani E, Saitou M, Wakayama T
  • Journal Title: Dev Biol Volume: 346 Pages: 90-101
  • Peer Reviewed
• [Journal Article] Inhibition of Class IIb Histone Deacetylase Significantly Improves Cloning Efficiency in Mice (2010)
  • Author(s): Ono T., Li C., Mizutani E., Terashita Y., Yamagata K., and Wakayama T
  • Journal Title: Biol Reprod Volume: 83 Pages: 929-937
  • Peer Reviewed
• [Journal Article] Aberrant protein expression in the placenta of cloned mouse derived from embryonic stem cell (2010)
  • Author(s): Kim HR, Han RX, Wakayama T, Park CS, Jin DI
  • Journal Title: Placenta Volume: 31 Pages: 853-859
  • Peer Reviewed
• [Journal Article] Six-dimensional Live-cell Imaging for the Mouse Preimplantation Embryo That Does Not Affect Full-term Development (2009)
  • Author(s): Yamagata K, Suetsugu R, Wakayama T. Long-term
  • Journal Title: J Reprod Dev Volume: 55 Pages: 343-350
  • Peer Reviewed
• [Journal Article] Effect of Vanadate on the Chromatin Configuration in Pig GV-oocytes (2009)
  • Author(s): Bui HT, Hwang KC, Kim JH, VAN Thuan N, Wakayama T, Miyano T
  • Journal Title: J Reprod Dev Volume: 55 Pages: 367-372
  • Peer Reviewed
• [Journal Article] A signaling principle for the specification of the germ cell lineage in mice (2009)
  • Author(s): Ohinata Y, Ohta H, Shigeta M, Yamanaka K, Wakayama T, Saitou M
  • Journal Title: Cell Volume: 137 Pages: 571-584
  • Peer Reviewed
• [Journal Article] The histone deacetylase inhibitor scriptaid enhances nascent mRNA production and rescues full-term development in cloned inbred mice (2009)
  • Author(s): Van Thuan N, Bui HT, Kim JH, Hikichi T, Wakayama S, Kishigami S, Mizutani E, Wakayama T
  • Journal Title: Reproduction Volume: 138 Pages: 309-317
  • Peer Reviewed
• [Journal Article] Assessment of chromosomal integrity using a novel live-cell imaging technique in mouse embryos produced by intracytoplasmic sperm injection (2009)
  • Author(s): Yamagata K, Suetsugu R, Wakayama T
  • Journal Title: Hum. Reprod Volume: 24 Pages: 2490-2499
  • Peer Reviewed
• [Journal Article] Establishment of trophoblast stem cell lines from somatic cell nuclear-transferred embryos (2009)
  • Author(s): Oda, M. Tanaka, S. Yamazaki, Y. Ohta, H. Iwatani, M. Suzuki, M. Ohgane, J. Hattori, N. Yanagimachi, R. Wakayama, T. Shiota, K
  • Journal Title: Proc Natl Acad Sci U S A Volume: 106 Pages: 16293-16297
  • Peer Reviewed
• [Journal Article] Effect of volume of oocyte cytoplasm on embryo development after parthenogenetic activation, intracytoplasmic sperm injection, or somatic cell nuclear transfer (2008)
  • Author(s): Wakayama S, Kishigami S, Thuan NV, Ohta H, Hikichi T, Mizutani E, Bui HT, Miyake M, and Wakayama T
  • Journal Title: Zygote Volume: 16 Pages: 211-222
  • Peer Reviewed
• [Journal Article] Nuclear Transfer Alters the DNA Methylation Status of Specific Genes in Fertilized And Parthenogenetically Activated Mouse Embryonic Stem Cells (2008)
  • Author(s): Hikichi T, Kohda T, Wakayama S, Ishino F, and Wakayama T
  • Journal Title: Stem Cells Volume: 26 Pages: 783-788
  • Peer Reviewed
• [Journal Article] The Birth of Mice from Testicular Spermatozoa Retrieved from Frozen Testicular Sections (2008)
  • Author(s): Ohta H, Sakaide Y, and Wakayama T
  • Journal Title: Biol Reprod Volume: 78 Pages: 807-811
  • Peer Reviewed
• [Journal Article] Therapeutic cloning in individual parkinsonian mice (2008)
  • Author(s): Tabar V, Tomishima M, Panagiotakos G, Wakayama S, Menon J, Chan B, Mizutani E, Al-Shamy G, Ohta H, Wakayama T, and Studer L
  • Journal Title: Nat. Med Volume: 14 Pages: 379-381
  • Peer Reviewed
• [Journal Article] X-chromosome inactivation in nuclear transfer ES cells (2008)
  • Author(s): Shibata S, Yokota T, and Wakayama T
  • Journal Title: Cytogenetic and Genome Research Volume: 121 Pages: 96-101
  • Peer Reviewed
• [Journal Article] Genome-Wide Analysis of Abnormal H3K9 Acetylation in Cloned Mice (2008)
  • Author(s): Suzuki T, Kondo S, and Wakayama T, Cizdziel PE, and Hayashizaki Y
  • Journal Title: PLoS One Volume: 3 Pages: e1905
  • Peer Reviewed
• [Journal Article] Propagation of senescent mice using nuclear transfer embryonic stem cell lines (2008)
  • Author(s): Mizutani E, Ono T, Li Chong, Maki-Suetsugu R, Wakayama T
  • Journal Title: Genesis Volume: 46 Pages: 478-483
  • Peer Reviewed
• [Journal Article] Nuclear transfer preserves the nuclear genome of freeze-dried mouse cells (2008)
  • Author(s): Ono T., Mizutani E., Li C., Wakayama T
  • Journal Title: J. Reprod Dev Volume: 54 Pages: 486-491
  • Peer Reviewed
• [Journal Article] The cytoplasm of mouse germinal vesicle (GV) stage oocytes can enhance somatic cell nuclear reprogramming (2008)
  • Author(s): Bui HT., Wakayama S., Kishigami S., Thuan NV. Wakayama T
  • Journal Title: Development Volume: 135 Pages: 3935-3945
  • Peer Reviewed
• [Journal Article] Production of healthy cloned mice from bodies frozen at -20℃ for 16 years, (2008)
  • Author(s): Wakayama S, Ohta H, Hikichi T, Mizutani E, Iwaki T, Kanagawa O, Wakayama T
  • Journal Title: Proc Natl Acad Sci U S A Volume: 105 Pages: 17318-17322
  • Peer Reviewed
• [Journal Article] An epigenetic aberration increased in intergenic regions of cloned mice (2008)
  • Author(s): Nishida H, Kondo S, Suzuki T, Tsujimura Y, Komatsu S, Wakayama T, Y Hayashizaki
  • Journal Title: Mammalian Genome Volume: 19 Pages: 667-674
  • Peer Reviewed
• [Book] Nuclear transfer in the mouse oocyte In "Mammalian Oocyte Regulation" Ed. by Hayden Homer (Humana press, USA) (2013)
  • Author(s): Mizutani E. Ogura A. and Wakayama T
  • Total Pages: 957:285-300
  • Publisher: Methods in Molecular Biology
• [Book] Cloned mice from Adults Stem Cells. In "Advanced in Molecular Biology and Medicine: Stem cells (2013)
  • Author(s): Obokata, H., Wakayama T
  • Total Pages: 209-232
  • Publisher: From Biology to Therapy" Vol.1 (Ed. Robert A. Meyers: Wiley-Blackwell Press)
• [Book] Cloned mice from embryonic stem cells. In "Advanced in Molecular Biology and Medicine: Stem cells (2013)
  • Author(s): Li C., Wakayama S. and Wakayama T
  • Total Pages: 233-254
  • Publisher: From Biology to Therapy" Vol.1 (Ed. Robert A. Meyers: Wiley-Blackwell Press)
• [Book] 体細胞核移植クローン 卵子学(森崇英総編集) (2011)
  • Author(s): 若山清香、若山照彦
  • Total Pages: 63-66
  • Publisher: 京都大学学術出版会
• [Book] Nuclear transfer ES cells as a new tool for basic biology In: Stem Cells & Regenerative Medicine: From Molecular Embryologyto Tissue Engineering. (ed. Krishnarao Appasani Raghu K. Appasani) (2010)
  • Author(s): Wakayama S. Mizutani E. and Wakayama T
  • Total Pages: 351-369
  • Publisher: Humana Press, USA
• [Book] Mouse cloning by nuclear transfer. In: Advanced protocols for animal transgenesis. (ed. Shirley Pease, Thomas L. Saunders) (2010)
  • Author(s): Wakayama S. Thuan NV. and Wakayama T
  • Total Pages: 267-290
  • Publisher: Springer New York
• [Book] クローンマンモスへの道-クローン技術最前線の技術における発生・再生医療技術を探る(単行本) (2009)
  • Author(s): 若山照彦
  • Publisher: アドスリー社 東京
• [Book] Generation of mice from embryonic stem cells using tetraploid embryos as hosts. In: Methods in Bioengineering. (ed. Biju Parekkadan and Martin L. Yarmush) (2009)
  • Author(s): Ohta H. and Wakayama T
  • Total Pages: 39-48
  • Publisher: Artech House. London
• [Book] Somatic cell nuclear transfer in the mouse. In: Microinjection: Methods and Applications (ed. David J. Carroll) (2009)
  • Author(s): Kishigami S, and Wakayama T
  • Total Pages: 518:207-218
  • Publisher: (Humana Press, USA), Methods in Molecular Biology
• [Book] Cloning of ES cells and mice by nuclear transfer. In: Gene Knockout Protocols ed. Ralf Kuehn, Wolfgang Wurst (Humana Press, USA) (2009)
  • Author(s): Wakayama S, Kishigami S, Wakayama T
  • Total Pages: 530:251-265
  • Publisher: Methods in Molecular Biology
• [Book] Establishment of individual-specific ES cells from adult somatic cells by nuclear transfer. In: Stem Cell Applications in Diseases (ed. Mikkel L. Sorensen) (2008)
  • Author(s): Wakayama S, and Wakayama T
  • Total Pages: 189-214
  • Publisher: Nova Science Publishers, Inc. New York USA
• [Book] Epigenetic remodeling and developmental potentials after intracytoplasmic injection of spermatozoa and spermatids. In: Genetic and Epigenetic Control of Mammalian Germ Cell Development and Function (ed. Masami Nozaki) (2008)
  • Author(s): Kishigami S, Ohta H, and Wakayama T
  • Total Pages: 133-152
  • Publisher: Research Signpost. Kerala, India
• [Remarks]
  • URL: http://www.bt.yamanashi.ac.jp/modules/kenkyu/index.php?cat_id=5