Design of Soft-interface with Biofunctional Branched Polymers

2010 Fiscal Year Self-evaluation Report

• Project Area: Molecular Soft-Interface Science
• Project/Area Number: 20106003
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Kyushu University (2010-2012); Japan Advanced Institute of Science and Technology (2008-2009)
• Principal Investigator: MIURA Yoshiko Japan Advanced Institute of Science and Technology, 大学院・工学研究院・化学工学部門 (00335069)
• Project Period (FY): 2008 – 2012
• Keywords: デンドリマー / 界面 / 糖鎖 / タンパク質 / 分子認識

Research Abstract

本研究では精密な高分子を利用して、バイオインターフェースを創製し、生体間相互作用を制御することを目的とする。特にタンパク質を認識する分子として糖鎖を取り上げる。
(1)糖鎖を活用した樹状高分子バイオインターフェースの創製
細胞表面の生理活性糖鎖は生命の認識信号として機能しているこのことから、精密な高分子である、デンドリマーを利用した糖鎖の空間配置を行い、タンパク質との相互作用の解析、制御、生体機能の制御を行う界面の創製を行う。
(2)樹状高分子高分子を用いた界面の機能制御
デンドリマーを固定化した界面はポリマーブラシのミミック構造となっている。そこで、デンドリマー固定化界面を利用することで、精密ブラシ構造を作製し、物性、生体機能性を明らかにしていく。また、デンドリマーをテンプレートとすることで、ポリマーや分子のブラシ密度を制御した材料の検討を行う。

Research Products

• [Journal Article] Bioinert surface to proteinadsorption with higher generation ofdendrimer SAMs (2011)
  • Author(s): Erino Matsumoto, Tomohiro Fukuda,Yoshiko Miura
  • Journal Title: Colloids Surf.B-2011. 84 Pages: 280-284
• [Journal Article] Aggregation of Alzheimer Amyloid β Peptide(1-42) on the Multivalent Sulfonated Sugar Interface (2010)
  • Author(s): Tomohiro Fukuda, Erino Matsumoto,Shunsuke Onogi, Yoshiko Miura
  • Journal Title: Bioconjugate Chem 21 Pages: 1079-1086
• [Journal Article] Peculiar wettability based on orientational change of self-assembled hemispherical PAMAM dendrimer-layer. (2010)
  • Author(s): Tomohiro Fukuda, Erino Matsumoto,Nobuhiko Yui , Yoshiko Miura
  • Journal Title: Chem. Lett. 39 Pages: 923-925
• [Journal Article] Interaction Analyses of Amyloid beta Peptide (1-40) with Glycosaminogly can Model Polymers Bull. (2010)
  • Author(s): Yoshiko Miura, Hikaru Mizuno
  • Journal Title: Chem. Soc.Jpn. 83 Pages: 1004-1009
• [Journal Article] Biological specificrecognition of glycopoly mermodifiedinter faces by RAFT living radical polymerization (2010)
  • Author(s): Masayuki Toyoshima,Tomoyuki. Oura,Tomohiro Fukuda, Erino Matsumoto, Yoshiko Miura
  • Journal Title: Polymer J. 42 Pages: 172-178
• [Journal Article] Dendritic sugar microarrays by Click Chemistry (2009)
  • Author(s): Tomohiro Fukuda, Shunsuke Onogi, Yoshiko Miura
  • Journal Title: Thin Solid Films 518 Pages: 880-888
• [Journal Article] A Specific Inhibitory Effect of Multivalent Trehalose toward Amyloidbeta(1-40) Aggregation
  • Author(s): Masaya Wada, Yuta Miyazawa, Yoshiko Miura
  • Journal Title: Polym. Chemaccepted.
• [Presentation] Control of amyloidosis on the multivalent sulfonate dsugar (2010)
  • Author(s): Tomohiro Fukuda, Erino Matsumoto,Shunsuke. Onogi, Yoshiko Miura
  • Organizer: Pacifichem
  • Place of Presentation: Hawaii
  • Year and Date: 20101200
• [Presentation] Dendritic sugar interface and biological functionality (2010)
  • Author(s): Yoshiko Miura
  • Organizer: Pacifichem
  • Place of Presentation: Hawaii
  • Year and Date: 20101200
• [Presentation] Interaction of glycosaminogly can model polymers with amyloid beta peptide (2009)
  • Author(s): Yoshiko Miura, Hikaru Mizuno
  • Organizer: 238th ACS National Meeting
  • Place of Presentation: Washington DC(USA)
  • Year and Date: 20090800
• [Presentation] Glyco-nanomaterials for Biosensing (2009)
  • Author(s): Yoshiko Miura
  • Organizer: WCG
  • Place of Presentation: Fusan (China)
  • Year and Date: 20090000
• [Presentation] Glyco-Nano Interface (2008)
  • Author(s): Y. Miura
  • Organizer: IUMRS
  • Place of Presentation: Nagoya
  • Year and Date: 20081200
• [Presentation] Dendritic Sugar-Microarrays by Click Chemistry (2008)
  • Author(s): T. Fukuda, S. Onogi, Y. Miura
  • Organizer: ICNME
  • Place of Presentation: Kobe
  • Year and Date: 20081200
• [Presentation] The monolayer of a-Man via Si-Cbond formation and protein recognition (2008)
  • Author(s): Koji Funato, Naoto Shirahata, Yoshiko Miura
  • Organizer: ICNME
  • Place of Presentation: Kobe
  • Year and Date: 20080000
• [Book] バイオマテリアルの基礎(糖質機能界面) (2010)
  • Author(s): 三浦佳子
  • Total Pages: 156-160
  • Publisher: 日本医学館
• [Book] 特殊形状高(分子、ソフトマター) (2009)
  • Author(s): 三浦佳子
  • Total Pages: 95-104
  • Publisher: 丸善
• [Book] 複合糖質の化学と最新応用技術(糖鎖材料を用いたアルツハイマー病へのアプローチ) (2009)
  • Author(s): 三浦佳子
  • Total Pages: 285-292
  • Publisher: シーエムシー出版
• [Patent(Industrial Property Rights)] 表面処理剤および表面処理方法 (2009)
  • Inventor(s): 松本絵里乃, 福田知博, 三浦佳子
  • Industrial Property Rights Holder: 北陸先端科学技術大学院大学
  • Industrial Property Number: 特願2009-200368
  • Filing Date: 2009-08-31