2012 Fiscal Year Final Research Report
3-Dimensional Nano Structured Interface for Detecting Carbohydrate and Protein Weak Interactions and its Dynamic Response Analysis
| Project Area | Molecular Soft-Interface Science |
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| Project/Area Number |
20106008
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
SATO Yukari 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 上級主任研究員 (40357132)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIOKA Kyoko 産業技術総合研究所, バイオメディカル研究部門・ナノバイオデバイス研究グループ, 主任研究員 (50358321)
TANAKA Mutsuo 産業技術総合研究所, バイオメディカル研究部門, バイオ界面研究グループ長 (70344108)
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| Project Period (FY) |
2008 – 2012
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| Keywords | ソフト界面 / 分子認識 / 自己組織化膜 / 糖 / レクチン / 表面プラズモン共鳴 / 金電極 |
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| Research Abstract |
Carbohydrate-protein interactions are not strong but they are very important in terms of specific recognition in vital functions. Although it is very easy for thick polyvalent layers to interact with target molecules, the thickness, bulkiness, and heterogeneity of the reaction layers pose serious problems in terms of quantitative analysis and realizing rapid reactions. It will be a challenge to form a thin layer with a polyvalent recognition ability. We investigated the processing of a nanostructured recognition site for sensing lectin, Concanavalin A (Con A), by using self-assembled monolayers (SAMs) consisting of artificial carbohydrate and OH terminated filling molecules formed on a gold substrate. We prepared carbohydrate ligands for recognition of lectins/galectins and tri (ethylene glycol) terminated short alkanethiol molecules for constructing ultra thin protein resistant monolayers on a gold surface. Electrochemical active molecules were combined with carbohydrate terminated molecules, and the mixed monolayer was adopted to detect weak interaction between the carbohydrate and lectins much easily.
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[Remarks] [新聞報道]佐藤 縁、日刊工業新聞「分子認識に優れたソフト界面」2011年9月1日
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[Remarks] [受賞](1) Hot Articles Award, Analytical Sciences, vol.27, No.5.(2011) ("Bifunctional Tri(ethylene glycol) Alkanethiol Immunoassay of pg Level Lectin", Yukari Sato, et. al.)
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[Remarks] 2) 2012年電気化学会第一回女性躍進賞 受賞「分子認識ソフト界面の構築と膜構造および機能評価に関する研究」佐藤 縁
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