Elucidation and Application of Sequence-Specific Phenomena of DNA-grafted Nanoparticles

2010 Fiscal Year Self-evaluation Report

• Project Area: Molecular Soft-Interface Science
• Project/Area Number: 20106010
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: MAEDA Mizuo The Institute of Physical and Chemical Research, 前田バイオ工学研究室, 主任研究員 (10165657)
• Project Period (FY): 2008 – 2012
• Keywords: ソフトインターフェース / 核酸分子 / コンジュゲート材料 / ナノ粒子 / ポリ(N-イソプロピルアクリルアミド) / 生体機能材料 / 界面科学 / 分子認識

Research Abstract

二重鎖DNAを表層に密生させた高分子ミセル(DNA担持ナノ粒子)のコロイド安定性と電気泳動移動度が、DNA自由末端側の塩基対構造に明敏に応答することを発見した。自由末端に一塩基ミスマッチが存在すると、完全相補の場合と比べて、コロイド安定性と電気泳動移動度が著しく増大する。これは、DNA密生相とバルクとの界面における分子構造のわずかな変化がマクロでダイナミックな現象を誘起していることを意味する。本研究は、この特異な界面現象のメカニズムを分子レベルで解明し、新しいバイオ分析デバイスへ応用することを目的とする。具体的には、(1)新規高分子-DNAコンジュゲートの精密重合、(2)DNA担持ナノ粒子が示す非架橋型凝集の構造科学的観点からの解明、(3)新規バイオセンサー開発、に関する研究テーマの推進をした。

Research Products

• [Journal Article] Rapid Naked-eye Detection of Mercury Ion Based on Non-cross linking Aggregation of Double-stranded DNA-carrying Gold Nanoparticles (2011)
  • Author(s): N. Kanayama, T. Takarada, M. Maeda
  • Journal Title: Chem. Commun 47 Pages: 2077-2079
  • Peer Reviewed
• [Journal Article] RAFT-generated poly(N-isopropylacrylamide)-DNA block copolymers for temperature-responsive formation of polymer micelles (2011)
  • Author(s): K. Isoda, N. Kanayama, D. Miyamoto, T. Takarada, M. Maeda
  • Journal Title: Reactive & Functional Polymers 71 Pages: 367-371
  • Peer Reviewed
• [Journal Article] DNA-Functionalized Ther moresponsive Bioconjugates Synthesized via ATRP and Click Chemistry (2011)
  • Author(s): P. Pan, M. Fujita, W.Y. Ooi, K. Sudesh, T. Takarada, A. Goto, M. Maeda
  • Journal Title: Polymer 52 Pages: 895-900
  • Peer Reviewed
• [Journal Article] A Facile Method Towards Cyclic Assembly of Gold Nanoparticles Using DNA Template Alone (2010)
  • Author(s): T. Ohshiro, T. Zako, R. Wanatabe-Tamaki, T. Tanaka, M. Maeda
  • Journal Title: Chem. Commun. 46 Pages: 6132-6134
  • Peer Reviewed
• [Journal Article] Single-molecule Imaging of DNA Duplexes Immobilized on Surfaces with a Scanning Tunneling Microscope (2010)
  • Author(s): T. Ohshiro, M. Maeda
  • Journal Title: Chem. Commun 46 Pages: 2581-2583
  • Peer Reviewed
• [Presentation] Structural study on noncrosslinking aggregation of gold nanoparticles with DNA (2010)
  • Author(s): M. Fujita
  • Organizer: Pacifichem2010
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2010-12-06
• [Presentation] Unique colloidal behavior of DNA-carrying nanoparticles (2010)
  • Author(s): Mizuo Maeda
  • Organizer: International Conference on Nanoscopic Colloid and Surface
  • Place of Presentation: Chiba, Japan
  • Year and Date: 2010-09-22
• [Presentation] DNA-co-poly(N-isopropylacryl amide) ther moresponsive bioconjugates : Preparation and self-assembly (2010)
  • Author(s): 潘鵬挙, 藤田雅弘, 後藤淳, 宝田徹, 前田瑞夫
  • Organizer: 第59回高分子討論会
  • Place of Presentation: 札幌市
  • Year and Date: 2010-09-16
• [Presentation] Structural Analysis of Non-crosslinking Aggregation of Au Nanoparticles with DNA (2009)
  • Author(s): M. Fujita
  • Organizer: 3rd International Symposium on Interdisciplinary Materials Science for Biomaterials Science
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2009-04-24
• [Presentation] DNA Conjugate Polymers and Nanoparticlels (2009)
  • Author(s): M. Maeda
  • Organizer: 14th International Symposium on Recent Advances in Drug Delivery Systems
  • Place of Presentation: Utah, USA
  • Year and Date: 2009-02-16