2012 Fiscal Year Final Research Report
Elucidation and application of sequence-specific phenomena of DNA-grafted nanoparticles
| Project Area | Molecular Soft-Interface Science |
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| Project/Area Number |
20106010
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
MAEDA Mizuo 独立行政法人理化学研究所, 前田バイオ工学研究室, 主任研究員 (10165657)
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| Co-Investigator(Kenkyū-buntansha) |
TAKARADA Tohru 独立行政法人理化学研究所, 前田バイオ工学研究室, 専任研究員 (30336010)
FUJITA Masahiro 独立行政法人理化学研究所, 前田バイオ工学研究室, 専任研究員 (50342845)
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| Project Period (FY) |
2008 – 2012
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| Keywords | ソフトインターフェース / 核酸分子 / コンジュゲート材料 / ナノ粒子 / ポリ(N-イソプロピルアクリルアミド) / 生体機能材料 / 遺伝子診断 / 界面科学 |
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| Research Abstract |
Nanoparticles densely grafted with double-stranded DNA having a terminal single-base mismatch between the DNA layer and the disperse medium exhibit significantly high colloidal stability compared to those with fully matched DNA. Measurements of small angle X-ray scattering patterns at SPring-8 and force curves by atomic force microscopy revealed that this unique property stems from the fact that steric repulsive force induced by micro-Brownian motion of the terminal bases surpasses van der Waals attractive force between the hydrophobic cores. The elucidation of the mechanism led us to rationally design the surface structure of nanoparticles for the reliable gene mutation assay and the colloidal logic circuits.
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