Construction of High-performance Biointerface by Hybrid of Biomolecules with Synthetic Polymers

2010 Fiscal Year Self-evaluation Report

• Project Area: Molecular Soft-Interface Science
• Project/Area Number: 20106011
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: University of Tsukuba
• Principal Investigator: NAGASAKI Yukio University of Tsukuba, 大学院・数理物質科学研究科, 教授 (90198309)
• Project Period (FY): 2008 – 2012
• Keywords: バイオインターフェース

Research Abstract

基材表面に生体分子を固定し、目的の相互作用だけを的確に認識させることは必ずしも容易ではなく、様々な工夫がなされているのが現状である。申請者らは、異なる長さを有するPEG鎖によるブラシが極めて高いタンパク質非特異吸着抑制効果を示すことを示した。さらに、DNAや抗体とPEGブラシを共固定した表面が、非特異吸着を抑制するだけでなく、DNA や抗体の認識能自身を向上させることを見いだした。このように生体分子とPEG ブラシとを共固定する表面密生層の構築は、これまで達し得なかった生体分子自身の性能を100%引き出す可能性を有する。本研究では生体分子とPEGの表面密生層の設計・作製・評価を行うだけでなく、バイオ界面のナノエンジニアリング概念を創成してきた。

Research Products

• [Journal Article] Improvement of the Thermal Stability of Streptavidin Immobilized on Magnetic Beads by the Construction of a Mixed-poly(ethylene glycol) Tethered-chain Layer (2011)
  • Author(s): Kubota Masaki, Yoshimoto Keitaro, Yuan Xiaofei, Nagasaki Yukio
  • Journal Title: Polymer Journal in press Pages: DOI:10.1038/pj.
  • Peer Reviewed
• [Journal Article] Direct Observation of Adsorption-Induced Inactivation of Antibody Fragments Surrounded by Mixed-PEG Layer on a Gold Surface (2010)
  • Author(s): Yoshimoto Keitaro, Nishio Motohiko, Sugasawa Hiroaki, Nagasaki Yukio
  • Journal Title: Journal of the American Chemical Society 132(23) Pages: 7982-7989
  • Peer Reviewed
• [Journal Article] Efficient Inhibition of Interfacial Nonspecific Interaction to Create Practically Utilizable High Ferritin-Response Immunolatex (2009)
  • Author(s): Yuan Xiaofei, Fabregat Dolca, Yoshimoto Keitaro, Nagasaki Yukio
  • Journal Title: Analytical Chemistry 81 Pages: 10097-10105
  • Peer Reviewed
• [Journal Article] Non-Fouling Character of Poly[2-(methacryloyloxy)ethyl Phosphorylcholine] Modified Gold Surfaces Fabricated by the "Grafting to" Method : Comparison of its Protein Resistance with Poly(ethylene glycol)Modified Gold Surfaces. (2009)
  • Author(s): Yoshimoto Keitaro, Hirase Takumi, Madsen Jeppe, Armes Steven P., Nagasaki Yukio
  • Journal Title: Macromolecular Rapid Communication 30(24) Pages: 2136-2140
  • Peer Reviewed
• [Journal Article] Studies on the Adsorption Property and Structure of Polyamine-Ended Poly(ethylene glycol)Derivatives on a Gold Surface by Surface Plasmon Resonance and Angle-resolved X-ray Photoelectron Spectroscopy. (2009)
  • Author(s): Yoshimoto Keitaro, Nozawa Makiko, Matsumoto Shinya, Echigo Takuya, Nemoto Seiko, Hatta Tamao, Nagasaki Yukio
  • Journal Title: Langmuir 25(20) Pages: 12243-12249
  • Peer Reviewed
• [Journal Article] Spheroid Array of Fetal Mouse Liver Cells Constructed on a PEG-gel Micropatterned Surface : Upregulation of Hepatic Functions by Co-Culture with Nonparenchymal Liver Cells. (2009)
  • Author(s): Kojima Ryota, Yoshimoto Keitaro, Miyoshi Hirotoshi, Nagasaki Yukio
  • Journal Title: Lab on a Chip 9 Pages: 1991-1993
  • Peer Reviewed
• [Journal Article] Inverted Pattern Formation of Cell Microarrays on Poly(ethylene glycol)(PEG)Gel Patterned Surface and Construction of Hepatocyte Spheroids on Unmodified PEG Gel Microdomains. (2009)
  • Author(s): Yoshimoto Keitaro, Ichino Masahiro, Nagasaki Yukio
  • Journal Title: Lab on a Chip 9 Pages: 1286-1289
  • Peer Reviewed
• [Journal Article] High-performance Immunolatex Possessing A Mixed-PEG/Antibody Co-immobilized Surface : High Sensitive Ferritin Immunodiagnostics. (2009)
  • Author(s): Yuan Xiaofei, Yoshimoto Keitaro, Nagasaki Yukio
  • Journal Title: Analytical Chemistry 81(4) Pages: 1549-1556
  • Peer Reviewed
• [Journal Article] Binding Enhancement of Antigen-Functionalized PEGylated Gold Nanoparticles onto Antibody-Immobilized Surface by Increasing the Functionalized Antigen using α-sulfanyl-ω -amino-PEG. (2008)
  • Author(s): Yoshimoto Keitaro, Hoshino Yuki, Ishii Takehiko, Nagasaki Yukio
  • Journal Title: Chemical Communications Pages: 5369-5371
  • Peer Reviewed
• [Journal Article] Facile Construction of Sulfanyl-terminated Poly(ethylene glycol)-brushed Layer on a Gold Surface for Protein Immobilization by the Combined Use of Sulfanyl-Ended Telechelic and Semi-Telechelic Poly(ethylene glycol)s. (2008)
  • Author(s): Yoshimoto Keitaro, Hirase Takumi, Nemoto Seiko, Hatta Tamao, Nagasaki Yukio
  • Journal Title: Langmuir 24 Pages: 9623-9629
  • Peer Reviewed
• [Presentation] Design of antibody/PEG hybrid biointerface for obtaining high-performance immunolatex (2010)
  • Author(s): Xiaofei Yuan, Dolca Fabregat, Keitaro Yoshimoto, Yukio Nagasaki
  • Organizer: Gordon Research Conferences(GRC)-Biointerface Science
  • Place of Presentation: Les Diablerets, Switzerland.
  • Year and Date: 2010-09-08
• [Presentation] Surface engineering of antibody/PEG hybrid for design of high-performance immunolatex (2010)
  • Author(s): Xiaofei Yuan, Dolca Fabregat, Keitaro Yoshimoto, Yukio Nagasaki
  • Organizer: IVC-18/ICN+T2010/ICSS-14/VASSCAA-5
  • Place of Presentation: Beijing, China.
  • Year and Date: 2010-08-26
• [Presentation] Hybridization of synthetic polymers and biopolymers for creation of high-performance biointerface (2009)
  • Author(s): Nagasaki Yukio
  • Organizer: Nano Europe 2009
  • Place of Presentation: Rapperswil, Switzerland
  • Year and Date: 2009-11-25
• [Patent(Industrial Property Rights)] 免疫ラテックス粒子及びその製造方法 (2009)
  • Inventor(s): 長崎幸夫, 吉本敬太郎, 原暁非, Dolca Fabrigat
  • Industrial Property Rights Holder: 筑波大学
  • Industrial Property Number: 特願,2009-007846
  • Filing Date: 2009-01-16
• [Patent(Industrial Property Rights)] 混合ポリエチレングリコールと抗体が共固定化された表面を所有する免疫ラテックス粒子及びその製造方法 (2009)
  • Inventor(s): 長崎幸夫, 原暁非, 吉本敬太郎
  • Industrial Property Rights Holder: 筑波大学
  • Industrial Property Number: 特願,2009-007846
  • Filing Date: 2009-01-16