Nano-fabication of Molecular Recognition Biointerfaces and Application to Cell Analysis Micro-biodevices

2010 Fiscal Year Self-evaluation Report

• Project Area: Molecular Soft-Interface Science
• Project/Area Number: 20106012
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: The University of Tokyo
• Principal Investigator: TAKAI Madoak The University of Tokyo, 大学院・工学系研究科, 教授 (40287975)
• Project Period (FY): 2008-04-01 – 2014-03-31
• Keywords: 生体材料 / ナノバイオ / 分子認識 / 細胞・組織 / 表面・界面物性

Research Abstract

再生医療や組織工学の発達において、細胞と材料間の相互作用を解析することは、細胞の材料表面への初期接着挙動が、増殖、伸展、分化およびアポトーシスなどの後の細胞挙動に大きな影響を与えるため重要である。細胞は、材料表面に吸着したタンパク質を介して表面と相互作用する。そこで、細胞-細胞間、細胞-マテリアル間の相互作用を独立に評価し、細胞とマテリアル間の相互作用を、一個の細胞を対象として解析するマイクロ流体デバイスの創製を目指す。細胞の接着/非接着を制御する分子認識バイオイターフェースとしては、ナノインプリント技術、リソグラフィー技術、自己組織化技術等を駆使して構築する。

Research Products

• [Journal Article] Significance of Antibody Orientation Unraveled : Well-Oriented Antibodies Recorded High Binding Affinity (2011)
  • Author(s): N.Tajima, M.Takai, K.Ishihara
  • Journal Title: Anal.Chem. 83 Pages: 1969-1976
  • Peer Reviewed
• [Journal Article] Stabilization of phospholipid polymer surface with three-dimensional nanometer-scaled structure for highly sensitive immunoassay (2010)
  • Author(s): K.Nishizawa, T.Konno, M.Takai, K.Ishihara
  • Journal Title: Colloids Surf.B : Biointerfaces 77 Pages: 263-269
  • Peer Reviewed
• [Journal Article] Protein adsorption and cell adhesion on cationic, neutral, and anionic 2-methacryloyloxyethyl phosphorylcholine copolymer surfaces (2009)
  • Author(s): Y.Xu, M.Takai, K.Ishihara
  • Journal Title: Biomaterials 30 Pages: 4930-4938
  • Peer Reviewed
• [Journal Article] Cell adhesion on phaseseparated surface of block copolymer composed of poly-(2-methacryloyloxyethyl phospholcholine) and poly(dimethylsilixane) (2009)
  • Author(s): J.-H.Seo, R.Matsuno, M.Takai, K.Ishihara
  • Journal Title: Biomaterials 30 Pages: 5530-5340
  • Peer Reviewed
• [Journal Article] Suppression of Protein Adsorption on a Charged Phospholipid Polymer Interface (2009)
  • Author(s): Y.Xu, M.Takai, K.Ishihara
  • Journal Title: Biomacromolecules 10 Pages: 267-274
  • Peer Reviewed
• [Presentation] The Effect of Nanophase-separated Hydrophric-Hydrophobic Copolymers to Cell Adhesion Behavior (2011)
  • Author(s): M.Takai, et al.
  • Organizer: BMMP-11
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2011-01-26
• [Presentation] A novel high-sensitive immunoassay interface with orientation-controlled antibodies (2010)
  • Author(s): M.Takai, et al.
  • Organizer: Pacifichem 2010
  • Place of Presentation: Honolulu Hawaii
  • Year and Date: 2010-12-16
• [Presentation] Microchip Immunoassay with 3D Nanostructured Phospholipids Polymer Biointerface (2009)
  • Author(s): M.Takai, et al.
  • Organizer: Micro-TAS 2009
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2009-11-03
• [Presentation] Microchip Analysis of Cellular Respiratory Activity on PDMS Membrane having Gas Permselective Property (2009)
  • Author(s): M.Takai, et al.
  • Organizer: Micro-TAS 2008
  • Place of Presentation: San Diego, USA
  • Year and Date: 2009-10-13
• [Presentation] Initial cell attachment behavior on various response surfaces analyzed by quartz crystal microbalance with dissipation (QCM-D) (2009)
  • Author(s): M.Takai, et al.
  • Organizer: 16th Congress on European Society for Biomaterials
  • Place of Presentation: Lausanne, Switzerland
  • Year and Date: 2009-09-09
• [Book] バイオチップ実用化ハンドブック(血液分析チップ) (2010)
  • Author(s): 高井まどか(共著)
  • Total Pages: 223-231
  • Publisher: NTS
• [Book] ソフトマター(細胞機能診断チップ) (2009)
  • Author(s): 高井まどか(共著)
  • Total Pages: 244-253
  • Publisher: 丸善
• [Book] 次世代医療のための高分子材料工学(微量血液診断チップ) (2008)
  • Author(s): 高井まどか(共著)
  • Total Pages: 263-373
  • Publisher: シーエムシー出版