2012 Fiscal Year Final Research Report
Evaluation and Application of Dynamic Phenomena on the Soft Interface between Ligand-Immobilized Suraface and Cell Surface
| Project Area | Molecular Soft-Interface Science |
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| Project/Area Number |
20106014
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
YAMAOKA Tetsuji 独立行政法人国立循環器病研究センター, 研究所, 部長 (50243126)
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| Co-Investigator(Kenkyū-buntansha) |
MAHARA Atsushi 独立行政法人国立循環器病研究センター, 研究所, 研究員 (80416221)
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| Project Period (FY) |
2008 – 2012
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| Keywords | 細胞ローリング / 抗体 / 幹細胞 / 高分子界面 / スルホベタイン |
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| Research Abstract |
We successfully developed a stem cell rolling column with a soft-interface bearing anti cell surface marker molecules antibody. A clued mesenchymal stem cell suspension was separated using these column and two different subpopulation of MSC was discovered in CD34 low and high franctions. We came up with a big problem about the non-specific cell entrapment on the antibody-immobilized column, and it reduces the efficiency of specific cell purification based on cell-rolling. To overcome this problem, newly modified surfaces were designed and evaluated for the cell-rolling surface. Hydrophilic polymer grafting, silane coupling reagents, and SAM on Au were selected and evaluated for the cell-rolling surfaces. As the results, zwitterionic telomer brush containing 2-methacryloyloxyethyl phosphorylcholine (MPC) efficiency suppressed the non-specific interaction between cells and antibody-immobilized surface. Finally, poly[MPC-co-n-butyl mechacrylate (nBMA)-co-N-vinylformamide (NVF)] copolymers were found to be very effective to suppress the interaction and achieved the very stable cell rolling and excellent cell separation property.
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Research Products
(62 results)
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[Journal Article] The use of magnetic resonance cell tracking to monitor endothelial progenitor cells in a rat hindlimb ischemic model2012
Author(s)
Agudelo, C.A., Tachibana, Y., Huatado, A.F., Ose, T., Iida, H., and Yamaoka, T.
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Journal Title
Biomaterials
Volume: 33
Pages: 2439-2448
Peer Reviewed
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