2012 Fiscal Year Final Research Report
Development of time-resolved methods for detection of fluctuations on energy and structure and for elucidating reaction mechanism
| Project Area | Molecular Science of Fluctuations toward Biological Functions |
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| Project/Area Number |
20107003
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2008 – 2012
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| Keywords | 揺らぎ / 生体分子 / 反応 / 機能 |
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| Research Abstract |
Relationship between fluctuation and biological function has been elucidated by the time-resolved transient grating method, which has been developed in our group. By using this technique, we successfully discovered that the flexible loop region of a blue light sensor FKF1 is important for the function. The volume compressibilities of reaction intermediates of PixD were measured and demonstrated that the fluctuation is indeed enhanced during the reaction. Time-resolve heat capacities and thermal expansion coefficients were also measured for reaction intermediates which were directly related with the function. A new analytical method of a single molecular detection has been developed for obtaining high time resolved information of the fluctuation.
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