Construction of Highly Integrated π-Space on Artificial Cell Membranes and Its Nano-device Function

2012 Fiscal Year Final Research Report

• Project Area: Emergence of highly elaborated pai-space and its function
• Project/Area Number: 20108013
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: KIKUCHI Jun-ichi 奈良先端科学技術大学院大学, 物質創成科学研究科, 教授 (90153056)
• Co-Investigator(Kenkyū-buntansha): YASUHARA Kazuma 奈良先端科学技術大学院大学, 物質創成科学研究科, 助教 (90545716) ; TAHARA Keishiro 奈良先端科学技術大学院大学, 物質創成科学研究科, 助教 (50622297)
• Project Period (FY): 2008 – 2012
• Keywords: 人工細胞膜 / 自己組織化 / π空間 / ナノデバイス / セラソーム

Research Abstract

In this study, we designed the artificial cell membranes as functional nano-devices. The self-assembled artificial cell membranes were constructed with a combination of lipid molecules, such as phospholipids, peptide lipids and cerasome-forming lipids, and π-conjugated functional molecules. The resulting artificial cell membranes provided the highly elaborated π-spaces, which acted as nano-devices performing membrane trafficking and signal transduction.

Research Products

• [Journal Article] A Photo-triggerable Drug Carrier Based on Cleavage of PEG lipids by Photosensitiser-generated Reactive Singlet Oxygen (2013)
  • Author(s): C. Komeda, A. Ikeda, J. Kikuchi, N. Ishida-Kitagawa, H. Tatebe, K. Shiozaki, M. Akiyama
  • Journal Title: Org. Biomol. Chem Volume: Vol.11, No.16 Pages: 2567-2570
  • DOI: DOI:org/10.1039/c2ob27199k
  • Peer Reviewed
• [Journal Article] Dechlorination of DDT Catalyzed by Visible-Light-Driven System Composed of Vitamin B_<12> Derivative and Rhodamine B (2013)
  • Author(s): K. Tahara, K. Mikuriya, T. Masuko, J. Kikuchi, Y. Hisaeda
  • Journal Title: J. Porphyr. Phthalocya Volume: Vol.17, No.1-2 Pages: 135-141
  • DOI: DOI:10.1142/S1088424612501398
  • Peer Reviewed
• [Journal Article] Fusion-Triggered Switching of Enzymatic Activity on Artificial Cell Membrane (2012)
  • Author(s): M. Mukai, Y. Sasaki, J. Kikuchi
  • Journal Title: Sensors Volume: Vol.12, No.5 Pages: 5966-5977
  • DOI: DOI:10.3390/s120505966
  • Peer Reviewed
• [Journal Article] Intermolecular Communication on a Liposomal Membrane. Enzymatic Amplification of a Photonic Signal with Gemini Peptide Lipid as a Membrane-bound Artificial Receptor (2012)
  • Author(s): M. Mukai, K. Maruo, Y. Sasaki, J. Kikuchi
  • Journal Title: Chem. Eur. J Volume: Vol.18, No.11 Pages: 3258-3263
  • DOI: DOI:10.1002/chem.201103552
  • Peer Reviewed
• [Journal Article] Advantages and Potential of Lipid-Membrane- Incorporating Fullerenes Prepared by the Fullerene-Exchange Method (2012)
  • Author(s): Ikeda, M. Mori, K. Kiguchi, K. Yasuhara, J. Kikuchi, K. Nobusawa, M. Akiyama, M. Hashizume, T. Ogawa, T. Takeya
  • Journal Title: Chem. Asian J Volume: Vol.7, No.3 Pages: 605-613
  • DOI: DOI:10.1002/asia.201100792
  • Peer Reviewed
• [Journal Article] Liposomal Architecture Boosts Biocompatibility of Nanohybrid Cerasomes (2011)
  • Author(s): Y. Ma, Z. Dai, Y. Gao, Z. Cao, Z. Zha, X. Yue, J. Kikuchi
  • Journal Title: Nanotoxicology Volume: Vol.5, No.4 Pages: 622-635
  • DOI: DOI:10.3109/17435390.2010.546950
  • Peer Reviewed
• [Journal Article] Location of [60]Fullerene Incorporation in Lipid Membranes (2011)
  • Author(s): A. Ikeda, K. Kiguchi, T. Shigematsu, K. Nobusawa, J. Kikuchi, M. Akiyama
  • Journal Title: Chem. Commun Volume: Vol.47, No.44 Pages: 12095-12097
  • DOI: DOI:10.1039/c1cc14650e
  • Peer Reviewed
• [Journal Article] Remarkable Long-Term Stability of Cerasome as an Organic-Inorganic Hybrid Nano-Container for Water-Soluble Macromoleculaes (2011)
  • Author(s): T. Kawataki, K. Yasuhara, J. Kikuchi
  • Journal Title: Chem. Lett Volume: Vol.40, No.5 Pages: 461-463
  • DOI: DOI:10.1246/cl.2011.461
  • Peer Reviewed
• [Journal Article] Photoresponsive Liposomal Nanohybrid Cerasomes (2011)
  • Author(s): X. Liang, X. Yue, Z. Dai, J. Kikuchi
  • Journal Title: Chem. Commun Volume: Vol.47, No.16 Pages: 4751-4753
  • DOI: DOI:10.1039/c1cc00063b
  • Peer Reviewed
• [Journal Article] Specific Delivery of Transport Vesicles Mediated by Complementary Recognition of DNA Signals with Membrane-bound Oligonucleotide Lipids (2011)
  • Author(s): K. Yasuhara, Z. Wang, T. Ishikawa, J. Kikuchi, Y. Sasaki, S. Hiyama, Y. Moritani, T. Suda
  • Journal Title: Supramol. Chem Volume: Vol.23, No.3-4 Pages: 218-225
  • DOI: DOI:10.1080/10610278.2010.521835
  • Peer Reviewed
• [Journal Article] Formation and Regulation of Fullerene-incorporation in Liposomes under the Phase Transition Temperature (2011)
  • Author(s): A. Ikeda, Y. Kawai, J. Kikuchi, M. Akiyama, E. Nakata, Y. Uto, H. Hori
  • Journal Title: Org. Biomol. Chem Volume: Vol.9, No.8 Pages: 2622-2627
  • DOI: DOI:10.1039/c0ob01 030h
  • Peer Reviewed
• [Journal Article] Switching of the Enzymatic Activity Synchronized with Signal Recognition by an Artificial DNA Receptor on a Liposomal Membrane (2011)
  • Author(s): Y. Sasaki, M. Mukai, A. Kawasaki, K. Yasuhara, J. Kikuchi
  • Journal Title: Org. Biomol. Chem Volume: Vol.9, No.7 Pages: 2397-2402
  • DOI: DOI:10.1039/c0ob00918k
  • Peer Reviewed
• [Journal Article] Synthesis of Organic-Inorganic Hybrid Bicelles - Lipid Bilayer Nanodiscs Encompassed by Siloxane Surfaces (2011)
  • Author(s): K. Yasuhara, S. Miki, H. Nakazono, A. Ohta, J. Kikuchi
  • Journal Title: Chem. Commun Volume: Vol.47, No.16 Pages: 4691-4692
  • DOI: DOI:10.1039/c1cc10254k
  • Peer Reviewed
• [Journal Article] Self-Assembly Control of Water-Solubilised Single-Walled Carbon Nanotubes by Combination of Reduction and Ligand Exchange Reactions of Transition Metal Complexes (2011)
  • Author(s): K. Nobusawa, A. Ikeda, J. Kikuchi
  • Journal Title: Supramol. Chem Volume: Vol.23, No.1-2, Pages: 102-105
  • DOI: DOI:10.1080/10610278.2010.514905
  • Peer Reviewed
• [Journal Article] Stabilized Liposomal Nanohybrid Cerasomes for Drug Delivery Applications (2010)
  • Author(s): Z. Cao, Y. Ma, X. Yue, S. Li, Z. Dai, J. Kikuchi
  • Journal Title: Chem. Commun Volume: Vol.46, No.29 Pages: 5265-5267
  • DOI: DOI:10.1039/b926367e
  • Peer Reviewed
• [Journal Article] Effect of Phase Transition Temperature of Liposomes on Preparation of Fullerene-Encapsulated Liposomes by the Fullerene-exchange Reaction (2010)
  • Author(s): A. Ikeda, Y. Kawai, J. Kikuchi, M. Akiyama
  • Journal Title: Chem. Commun Volume: Vol.46, No.16 Pages: 2847-2849
  • DOI: DOI:10.1039/b926949e
  • Peer Reviewed
• [Journal Article] Effects of Surface Carboxylic Acid Groups of Cerasomes, Morphologically Stable Vesicles Having a Silica Surface, on Biomimetic Deposition of Hydroxyapatite in Body Fluid Conditions (2010)
  • Author(s): M. Hashizume, H. Horii, J. Kikuchi, M. Kamitakahara, C. Ohtsuki, M. Tanihara
  • Journal Title: J. Mater. Sci: Mater. Med Volume: Vol.21, No.1 Pages: 11-19
  • DOI: DOI:10.1007/s10856-009-3829-7
  • Peer Reviewed
• [Journal Article] Budding and Fission of Cationic Binary Lipid Vesicles Induced by the Incorporation of Pyranine (2010)
  • Author(s): Z. Wang, K. Yasuhara, H. Ito, M. Mukai, J. Kikuchi
  • Journal Title: Chem. Lett Volume: Vol.39, No.1 Pages: 54-55
  • DOI: DOI:10.1246/cl.2010.54
  • Peer Reviewed
• [Journal Article] Sensory Device Fabricated on a Liposome for Detection of Chemical Signals (2010)
  • Author(s): Y. Sasaki, Y. Shioyama, W.-J. Tian, J. Kikuchi, S. Hiyama, Y. Moritani, T. Suda, A Nano
  • Journal Title: Biotechnol. Bioeng Volume: Vol.105, No.1 Pages: 37-43
  • DOI: DOI: 10.1002/bit.22521
  • Peer Reviewed
• [Journal Article] Propagation and Amplification of Molecular Information using a Photo-responsive Molecular Switch (2009)
  • Author(s): M. Mukai, K. Maruo, J. Kikuchi, Y. Sasaki, S. Hiyama, Y. Moritani, T. Suda
  • Journal Title: Supramol. Chem Volume: Vol.21, No.3-4 Pages: 284-291
  • DOI: DOI:10.1080/10610270802468439DOI:10.1039/b905387e
  • Peer Reviewed
• [Journal Article] Efficient Fluorescence Resonance Energy Transfer in Highly Stable Liposomal Nanohybrid Cerasome (2009)
  • Author(s): Z.-F. Dai, W.-J. Tian, X.-L. Yue, Z.-Z. Zheng, J.-J. Qi, N. Tamai, J. Kikuchi
  • Journal Title: Chem. Commun Volume: No.15 Pages: 2032-2034
  • DOI: DOI:10.1039/b900051h
  • Peer Reviewed
• [Journal Article] Direct and Short-time Uptake of [70]Fullerene into the Cell Membrane using an Exchange reaction from a [70]Fullerene-γ- Cyclodextrin Complex and the Resulting Photodynamic Activity (2009)
  • Author(s): A. Ikeda, M. Matsumoto, M. Akiyama, J. Kikuchi, T. Ogawa, T. Takeya
  • Journal Title: Chem. Commun Volume: No.12 Pages: 1547-1549
  • DOI: DOI: 10.1039/b820768b
  • Peer Reviewed
• [Journal Article] Photodynamic Activity of C_<70> Caged within Surface-Cross-Linked Liposome (2009)
  • Author(s): A. Ikeda, M. Nagano, M. Akiyama, M. Matsumoto, S. Ito, M. Mukai, M. Hashizume, J. Kikuchi, K. Katagiri, T. Ogawa, T. Takeya
  • Journal Title: Chem. Asian Volume: Vol.4, No.1 Pages: 199-205
  • DOI: DOI:10.1002/asia.200800271
  • Peer Reviewed
• [Journal Article] APhoto-responsive Cholesterol Capable of Inducing a Morphological Transformation of the Liquid-Ordered Microdomain in Lipid Bilayers (2008)
  • Author(s): K. Yasuhara, Y. Sasaki, J. Kikuchi
  • Journal Title: Colloid Poly. Sci Volume: Vol.286,No.14-15 Pages: 1675-1680
  • DOI: DOI:10.1007/s00396-008-1936-5
  • Peer Reviewed
• [Presentation] Electrochemical Behavior of Biosensing Electrode Modified with Enzyme-Cerasome Composite (2012)
  • Author(s): Qiao Yun, Qian Zhang, Xi-Ming Song, K. Tahara, J. Kikuchi
  • Organizer: The International Symposium on Preparative Chemistry of Advanced Materials 2012
  • Place of Presentation: Shenyang, P.R. China
  • Year and Date: 2012-09-14
• [Presentation] Cerasomes as an Organic-Inorganic Nanohybrid Material (2012)
  • Author(s): J. Kikuchi
  • Organizer: The International Symposium on Preparative Chemistry of Advanced Materials 2012
  • Place of Presentation: Shenyang, P.R. China
  • Year and Date: 2012-09-14
• [Presentation] 生体に学ぶ:バイオミメティックマテリアルの創成と未来材料としての可能性 (2012)
  • Author(s): 菊池純一
  • Organizer: バイオミミクリ研究会
  • Place of Presentation: 大阪府守口市
  • Year and Date: 2012-08-22
• [Presentation] 分子通信:生物に学ぶ次世代型情報通信 (2012)
  • Author(s): 菊池純一
  • Organizer: 日本真空学会関西支部&日本表面科学会関西支部合同セミナー2012
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2012-07-06
• [Presentation] Cerasome-Mediated Gene Delivery to Primary Hippocampal Neurons (2012)
  • Author(s): K. Tahara, T. Moriuchi, A. Hirota, K. Yasuhara, T. Maeno, M. Toriyama, N. Inagaki, J. Kikuchi
  • Organizer: International Symposium on Macrocyclic and Supramolecular Chemistry
  • Place of Presentation: Otago, New Zeeland
  • Year and Date: 2012-02-01
• [Presentation] Gene Delivery by an Organic-Inorganic Hybrid Vesicle "Cerasome" (2012)
  • Author(s): K. Tahara, J. Kikuchi
  • Organizer: 12th International Symposium on Biomimetic Materials Processing
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2012-01-26
• [Presentation] Roles of a Chemical Signal Which Induces Budding and Fission of Binary Lipid Vesicles (2012)
  • Author(s): F. Hao, K. Tahara, K. Yasuhara, J. Kikuchi
  • Organizer: 12th International Symposium on Biomimetic Materials Processing
  • Place of Presentation: 愛知県名古屋市.
  • Year and Date: 2012-01-26
• [Presentation] Cerasomes: A New Family of Artificial Cell Membranes with Ceramic Surface (2011)
  • Author(s): J. Kikuchi
  • Organizer: 26th Philippine Chemistry Congress
  • Place of Presentation: Cebu, Philippine
  • Year and Date: 2011-04-13
• [Presentation] Release of Small Vesicles from a Giant Vesicle Triggered by a Chemical Signal (2011)
  • Author(s): H. Noriyasu, H. Ito, Z. Wang, K. Yasuhara, J. Kikuchi, S. Hiyama, Y. Moritani
  • Organizer: 11th International Symposium on Biomimetic Materials Processing
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2011-01-25
• [Presentation] 生体に学ぶ-バイオ・ナノハイブリッドマテリアルの創成と未来材料としての可能性 (2011)
  • Author(s): 菊池純一
  • Organizer: 表面科学技術研究会2011
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2011-01-20
• [Presentation] Budding and Fission of Artificial Cell Membrane Triggered by a Chemical Stimulus (2010)
  • Author(s): K. Yasuhara, Z. Wang, H. Ito, H. Noriyasu, J. Kikuchi
  • Organizer: The 2011 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2010-12-16
• [Presentation] Artificial Membrane Traffic System: Selective Propagation of Molecular Information Using Gemini Peptide Lipids as Molecular Switches (2010)
  • Author(s): K. Yasuhara, Z. Wang, S. Okuda, A. Hirota, J. Kikuchi, S. Hiyama, Y. Moritani
  • Organizer: International Symposium on Molecular Nanotechnology
  • Place of Presentation: 奈良県奈良市
  • Year and Date: 2010-12-01
• [Presentation] 人工細胞による情報伝達 (2010)
  • Author(s): 菊池純一
  • Organizer: 化学工学会第42回秋季大会
  • Place of Presentation: 京都府京都市
  • Year and Date: 2010-09-06
• [Presentation] 新ICT パラダイム創出を目指した人工細胞システムの構築 (2010)
  • Author(s): 菊池純一
  • Organizer: ポリマーフロンティア21
  • Place of Presentation: 東京都
  • Year and Date: 2010-06-11
• [Presentation] Bio-inspired Molecular Communication System Based on Molecular Recognition on Artificial Cell Membrane (2010)
  • Author(s): K. Yasuhara, J. Kikuchi
  • Organizer: 5th International Symposium on Macrocyclic and Supramolecular Chemistry
  • Place of Presentation: 奈良県奈良市
  • Year and Date: 2010-06-09
• [Presentation] Chemically-Triggered Budding and Fission of Artificial Cell Membrane as a Membrane Trafficking Model (2010)
  • Author(s): K. Yasuhara, H. Ito, Z. Wang, J. Kikuchi
  • Organizer: 5th International Symposium on Macrocyclic and Supramolecular Chemistry
  • Place of Presentation: 奈良県奈良市
  • Year and Date: 2010-06-09
• [Presentation] Budding and Fission of Lipid Vesicles Triggered by a Chemical Signal (2010)
  • Author(s): J. Kikuchi
  • Organizer: 10th International Symposium on Biomimetic Materials Processing
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2010-01-27
• [Presentation] Controlled Budding and Fission of Artificial Cell Membranes Induced by a Chemical Signal (2009)
  • Author(s): H. Ito, Z. Wang, K. Yasuhara, J. Kikuchi
  • Organizer: The 1st FAPS Polymer Congress
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2009-10-22
• [Presentation] Processing of Molecular Information on Lipid Bilayer Membranes Using Artificial Receptors (2009)
  • Author(s): A. Kawasaki, M. Mukai, K. Yasuhara, J. Kikuchi
  • Organizer: The 1st FAPS Polymer Congress
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2009-10-22
• [Presentation] Design of an Artificial Membrane Traffic System for Molecular Communication (2009)
  • Author(s): J. Kikuchi, Z.-H. Wang, K. Yasuhara, S. Hiyama, Y. Moritani, T. Suda
  • Organizer: 4th International Symposium on Macrocyclic and Supramolecular Chemistry
  • Place of Presentation: Maastricht, Netherlands
  • Year and Date: 2009-06-22
• [Book] 人工細胞膜の自己組織化能を利用する高次集積π 空間の構築とナノデバイス機能, 「高次π 空間の創発と機能開発」,赤坂健, 大須賀篤弘, 福住俊一, 神取秀樹 監修 (2013)
  • Author(s): 菊池純一,安原主馬,田原圭志朗
  • Total Pages: 140-145
  • Publisher: シーエムシー出版
• [Book] 人工細胞膜で創る分子デバイス, 「ひかりエネルギー革命 -グリーンフォトニクス-」,NAIST グリーンフォトニクス研究チーム 編著 (2012)
  • Author(s): 菊池純一
  • Total Pages: 106-111
  • Publisher: 化学同人
• [Book] 分子デバイスの開発とナノ・バイオサイエンスの開拓, 「ICガイドブック 未来を創る!半導体」, 電子情報技術産業協会編 (2012)
  • Author(s): 菊池純一
  • Total Pages: 188-191
  • Publisher: 産業タイムズ社,
• [Book] Transmission Electron Microscopy (TEM), in "Supramolecular Chemistry: From Molecules to Nanomaterials", Vol.2, P. A. Gale, J. W. Steed, eds., John Wiley & Sons (2012)
  • Author(s): J. Kikuchi, K. Yasuhara
  • Total Pages: 633-645
  • Publisher: Chichester
• [Book] 三次元集積化ナノハイブリッド材料としてのセラソーム,「ナノハイブリッド材料の開発と応用」 (2011)
  • Author(s): 橋詰峰雄, 佐々木善浩, 菊池純一
  • Total Pages: 134-144
  • Publisher: シーエムシー出版,
• [Book] Cerasomes: A New Family of Artificial Cell Membranes with Ceramic Surface, in "Advances in Biomimetics", A. George, ed (2011)
  • Author(s): J. Kikuchi, K. Yasuhara
  • Total Pages: 231-250
  • Publisher: InTech, Rijeka
• [Book] バイオミメティック超分子,「超分子サイエンス&テクノロジー」,国武豊喜 監修 (2009)
  • Author(s): 菊池純一
  • Total Pages: 169-173
  • Publisher: エヌ・ティー・エス