π space control of firefly luciferase

2012 Fiscal Year Final Research Report

• Project Area: Emergence of highly elaborated pai-space and its function
• Project/Area Number: 20108016
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Kyoto University
• Principal Investigator: NAKATSU Toru 京都大学, 大学院・薬学研究科, 准教授 (50293949)
• Project Period (FY): 2008 – 2012
• Keywords: 生物発光

Research Abstract

Firefly luciferase catalyzes the emission of yellow-green light and the emission color changes to red by the substitution of an amino acid. We determine the Tyr257, Asn231 and Arg220 mutants. Tyr257 mutants decreased the hydrophobic interaction in the active site. Asn231 and Arg220 mutants broak the hydrogen-bonding network of oxyluciferin binding region. In the result, it is supposed that the change of the emission color is caused by the energy loss of the excited oxyluciferin.

Research Products

• [Journal Article] rystallization and preliminary crystallographic analysis of two eukaryotic fructosyl peptide oxidases (2013)
  • Author(s): Ichiyanagi A, Hirokawa K, Gomi K, Nakatsu T, Kato H, Kajiyama N
  • Journal Title: Acta Crystallogr Sect F Struct Biol Cryst Commun Volume: 69(Pt 2) Pages: 130-3
  • DOI: doi:10.1107/S1744309112051445
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray crystallographic analysis of Pz peptidase B from Geobacillus collagenovorans MO-1 (2012)
  • Author(s): Nakano H, Hosokawa A, Tagawa R, Inaka K, Ohta K, Nakatsu T, Kato H, Watanabe K
  • Journal Title: Acta Crystallogr Sect F Struct Biol Cryst Commun Volume: 68(Pt 7) Pages: 757-9
  • DOI: doi:10.1107/S1744309112018969
  • Peer Reviewed
• [Journal Article] Structural basis for docking of peroxisomal membrane protein carrier Pex19p onto its receptor Pex3p (2010)
  • Author(s): Sato Y, Shibata H, Nakatsu T, Nakano H, Kashiwayama Y, Imanaka T, Kato H
  • Journal Title: EMBO J Volume: 29 Pages: 4083-4093
  • DOI: doi:10.1038/emboj.2010.293
  • Peer Reviewed
• [Journal Article] Deleting two C-terminal alpha-helices is effective to crystallize the bacterial ABC transporter Escherichia coli MsbA complexed with AMP-PNP (2010)
  • Author(s): Terakado K, Kodan A, Nakano H, Kimura Y, Ueda K, Nakatsu T, Kato H
  • Journal Title: Acta Crystallogr D Volume: 66 Pages: 319-323
  • DOI: doi:10.1111/j.1742-4658.2009.07072.x
  • Peer Reviewed
• [Journal Article] Functional role of the linker region in purified human P-glycoprotein (2009)
  • Author(s): Sato T, Kodan A, Kimura Y, Ueda K, Nakatsu T, Kato H
  • Journal Title: FEBS Journal Volume: 276 Pages: 3504-3516
  • Peer Reviewed
• [Journal Article] Structural basis for gibberellin recognition by its receptor GID1 (2008)
  • Author(s): Shimada A, Ueguchi-Tanaka M, Nakatsu T, Nakajima M, Naoe Y, Ohmiya H, Kato H, Matsuoka M
  • Journal Title: Nature Volume: 456 Pages: 520-523
  • DOI: doi:10.1038/nature07546
  • Peer Reviewed
• [Journal Article] Mechanism of efficient firefly bioluminescence via adiabatic transition state and seam of sloped conical intersection (2008)
  • Author(s): Chung LW, Hayashi S, Lundberg M, Nakatsu T, Kato H, Morokuma K
  • Journal Title: J Am Chem Soc Volume: 130 Pages: 12880-12881
  • DOI: doi:10.1021/ja8052464
  • Peer Reviewed
• [Presentation] ホタル由来ルシフェラーゼのオキシルシフェリンアナログ複合体の高分解能X線結晶構造解析 (2012)
  • Author(s): 寺角 香菜子, Naumov Panche, 五味 恵子, 梶山 直樹, 加藤 博章, 中津 亨
  • Organizer: 日本生化学会 2012年度年会
  • Place of Presentation: マリンメッセ福岡(福岡県)
  • Year and Date: 20121214-16
• [Presentation] Structural basis for color modulation mechanism of firefly luciferase bioluminescence (2012)
  • Author(s): Kanako Terakado, Ryosuke Yoshimune, Keiko Gomi, Naoki Kajiyama, Hideyuki Ikeuchi, Jun Hiratake, Hiroaki Kato and Toru Nakatsu
  • Organizer: 17th International Symposium on Bioluminescence and Chemiluminescence
  • Place of Presentation: Guelph Ontario, Canada
  • Year and Date: 20120528-0602
• [Presentation] ゲンジボタルルシファラーゼの蛍光色制御メカニズムの解明 (2011)
  • Author(s): 寺角香菜子、吉宗良祐、五味恵子、梶山直樹、池内秀幸、平竹潤、加藤博章、中津亨
  • Organizer: 日本結晶学会 2011年度年会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 20111124-25
• [Presentation] ゲンジボタルルシフェラーゼの発光色決定メカニズムの解明 (2011)
  • Author(s): 寺角 香菜子、吉宗 良祐、五味 恵子、梶山 直樹、池内 秀幸、平竹 潤、加藤 博章、中津 亨
  • Organizer: 日本農芸化学会2011年度年会
  • Place of Presentation: 京都女子大学(京都市)
  • Year and Date: 20110325-28
• [Presentation] ホタルルシフェラーゼの発光色決定メカニズムの解明 (2010)
  • Author(s): 吉宗良祐、寺角香菜子、加藤博章、中津亨
  • Organizer: 日本結晶学会平成22年度年会
  • Place of Presentation: 大阪大学コンベンションセンター(大阪府)
  • Year and Date: 20101203-05
• [Presentation] (2010)
  • Author(s): 寺角香菜子、吉宗良祐、五味恵子、梶山直樹、池内秀幸、平竹潤、加藤博章、中津亨
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
  • Year and Date: 20100616-18
• [Presentation] ホタルにおける発光制御メカニズム (2009)
  • Author(s): 中津亨
  • Organizer: 日本農芸化学会2008年度大会
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 20090327-29
• [Presentation] (2009)
  • Author(s): 寺角香菜子、中津亨
  • Organizer: ホタル・ルシフェラーセによるπ空間制御機構日本生物物理学会
  • Place of Presentation: アスティ徳島(徳島)
  • Year and Date: 2009-10-30
• [Book] 生体π空間の制御機構の解明と新機能開発ーホタルルシフェラーゼによるπ空間制御機構ー (2013)
  • Author(s): 寺角 香菜子、中津 亨
  • Total Pages: 223-228
  • Publisher: シーエムシー出版
• [Book] 高機能化ホタル・ルシフェラーゼ創製に向けて (2010)
  • Author(s): 中津亨
  • Total Pages: 81-88
  • Publisher: 日本結晶学会