Cytoplasmic RNA granules for intracellular mRNA localization and translational control

2010 Fiscal Year Self-evaluation Report

• Project Area: Diversity and asymmetry achieved by RNA program
• Project/Area Number: 20112002
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: NAKAMURA Akira The Institute of Physical and Chemical Research, 生殖系列研究チーム, チームリーダー (90323245)
• Project Period (FY): 2008 – 2012
• Keywords: ショウジョウバエ / 生殖質 / 生殖顆粒 / 生殖細胞 / RNA局在 / RNP / 翻訳制御 / 細胞骨格

Research Abstract

細胞の極性は,しばしばmRNAの細胞質内局在と,RNA局在と連携した翻訳制御によって確立される。このようなRNA局在と翻訳制御との連携機構の解明は,発生・細胞生物学上の最重要課題の1つである。しかし,その分子基盤はいまだ良くわかっていない。本研究では,細胞極性,RNA局在,翻訳制御,RNP動態制御を包括的に研究する上で理想的なモデル系の一つである,ショウジョウバエ生殖質形成を対象として,RNAレベルで行われる制御の普遍的分子基盤の実体解明を目指す。具体的には,ショウジョウバエ母性RNP複合体の新規構成タンパク質として同定したEdc3ホモログについて,突然変異体を作成し分子機能解析を進めた。また,生殖質の局在に異常を示す突然変異体の原因遺伝子としてCG8683(酵母mon2ホモログ)を同定し、その解析を進めた。

Research Products

• [Journal Article] Repression of early zygotic transcription in the germline. Curr (2010)
  • Author(s): Nakamura, A., Shirae-Kurabayashi, M., Hanyu-Nakamura, K.
  • Journal Title: Opin. Cell Biol 22 Pages: 709-714
  • Peer Reviewed
• [Journal Article] Membrane protein localization-dependent regulation by PI3K(III) and Rabenosyn-5 in Drosophila wing cells (2009)
  • Author(s): Abe, M., Setoguchi, Y., Tanaka, T., Awano, W., Takahashi, K., Ueda, R., Nakamura, A., Goto, S.
  • Journal Title: PLoS ONE 4 Pages: e7306
  • Peer Reviewed
• [Journal Article] Less is more : specification of the germline by transcriptional repression. (2009)
  • Author(s): Nakamura, A., Seydoux, G.
  • Journal Title: Development 135 Pages: 3817-3827
  • Peer Reviewed
• [Journal Article] Drosophila Mon2 couples Oskar-induced endocytosis with actin remodeling for cortical anchorage of the germ plasm
  • Author(s): Tanaka, T, Kato, Y., Hanyu-Nakamura, K., Matsuda, K. , Nakamura, A.
  • Journal Title: Development in press
  • Peer Reviewed
• [Journal Article] Roles of Drosophila Deltex in Notch receptor endocytic trafficking and activation
  • Author(s): Yamada, K., Fuwa, T., Ayukawa, T., Tanaka, T., Nakamura, A., Baron, M., Matsuno, K.
  • Journal Title: Genes Cells 16 Pages: 1365-2443
  • Peer Reviewed
• [Presentation] Repression of early zygotic transcription in Drosophila primordial germ cells (2010)
  • Author(s): Nakamura, A
  • Organizer: 2010 SDB-JSDB Joint Meeting (The 69th Annual Meeting of the Society of Developmental Biology), Satellite Symposium on "Germ Cells"
  • Place of Presentation: Albuquerque, New Mexico, USA(招待講演)
  • Year and Date: 20100805-20100809
• [Presentation] Roles of the endocytic pathway in the polarized transport and cortical anchoring of germ cell determinants in the Drosophila oocyte. (2010)
  • Author(s): Nakamura, A.
  • Organizer: The 2nd Joint Meeting of the French and Japanese Societies for Developmental Biology on "From Cells to Organs"
  • Place of Presentation: Paris, France(招待講演)
  • Year and Date: 20100526-20100528
• [Presentation] Repression of zygotic transcription and maternal RNA degradation in Drosophila primordial germ cells (2010)
  • Author(s): Nakamura, A
  • Organizer: The 19th CDB meeting on "RNA Sciences in Cell and Developmental Biology"
  • Place of Presentation: Kobe, Hyogo(招待講演)
  • Year and Date: 20100510-20100512
• [Presentation] ショウジョウバエoskarmRNAの局在と生殖質形成におけるエンドソーム経路を介したF-アクチン再編 (2009)
  • Author(s): 中村輝
  • Organizer: 第82回日本生化学会年会
  • Place of Presentation: ポートアイランド 神戸(招待講演)
  • Year and Date: 20091021-20091024
• [Presentation] Roles of the endocytic pathway in oskar mRNA localization and germ plasm anchoring. (2009)
  • Author(s): Nakamura, A.
  • Organizer: FASEB Summer Research Conferences on "Intracellular mRNA Transport and Localized Translation"
  • Place of Presentation: Saxtons River, Vermont, USA(招待講演)
  • Year and Date: 20090712-20090717
• [Remarks] ホームページ
  • URL: http://www.cdb.riken.jp/jp/02_research/0202_creative05.html