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2012 Fiscal Year Final Research Report

Regulation of Nucleo-cytoplasmic Distribution of RNAs

Planned Research

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Project AreaDiversity and asymmetry achieved by RNA program
Project/Area Number 20112007
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionKyoto University

Principal Investigator

OHNO Mutsuhito  京都大学, ウイルス研究所, 教授 (80201979)

Project Period (FY) 2008 – 2012
KeywordsRNA / 輸送 / 核 / 細胞質 / 識別
Research Abstract

We have shown that the tetramer of hnRNP C measures the length of the RNA polymerase II transcripts, like a molecular ruler, and classifies them into either mRNA or U snRNA according to their lengths. We have also revealed factors involved in the nuclear retention of pre-mRNAs in mammalian cells. Furthermore, we have obtained new insights in the area of RNA export.

  • Research Products

    (31 results)

All 2013 2012 2011 2010 2009 2008

All Journal Article (15 results) (of which Peer Reviewed: 15 results) Presentation (16 results)

  • [Journal Article] hDbr1 is a nucleocytoplasmic shuttling protein with a protein phosphatase-like motif essential for debranching activity2013

    • Author(s)
      Kataoka, N. Hagiwara, M. and Ohno, M
    • Journal Title

      Sci. Rep

      Volume: 3-1090 Pages: 1090(オンライン番号)

    • DOI

      doi:10.1038/srep01090

    • Peer Reviewed
  • [Journal Article] Size matters in RNA export2012

    • Author(s)
      Ohno, M
    • Journal Title

      RNA Biology

      Volume: 9(12) Pages: 1-5

    • DOI

      doi:10.4161/rna.22569

    • Peer Reviewed
  • [Journal Article] Mechanistic insights into human pre-mRNA splicing of human ultra-short introns: potential unusual mechanism identifies G-rich introns2012

    • Author(s)
      Sasaki-Haraguchi N, Shimada MK, Taniguchi I, Ohno M, Mayeda A
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 423(2) Pages: 289-94

    • DOI

      doi:10.1016/j.bbrc.2012.05.112

    • Peer Reviewed
  • [Journal Article] 40S subunit dissociation and proteasome-dependent RNA degradation in nonfunctional 25S rRNA decay2012

    • Author(s)
      Fujii,K., Sakata,T., Kitabatake,M. and Ohno, M
    • Journal Title

      The EMBO Journa

      Volume: 31 Pages: 2579-2589

    • DOI

      doi:10.1038/emboj.2012.85

    • Peer Reviewed
  • [Journal Article] HnRNP C tetramer measures RNA length to classify RNA polymerase II transcripts for export2012

    • Author(s)
      McCloskey,A. Taniguchi,I,. Shinmyozu, K, and Ohno, M
    • Journal Title

      Science

      Volume: 30 Pages: 1643-1646

    • Peer Reviewed
  • [Journal Article] Multiple factors in the early splicing complex are involved in the nuclear retention of pre-mRNAs in mammalian cells2011

    • Author(s)
      Takemura,R., Takeiwa,T,. McCloskey, A.,Taniguchi,I. and Ohno, M
    • Journal Title

      Genes Cells

      Volume: 16(10) Pages: 1035-49

    • DOI

      DOI:10.1111/j.1365-2443.2011.01548

    • Peer Reviewed
  • [Journal Article] Specific Y14 domains mediate its nucleo- cytoplasmic shuttling and association with spliced mRNA2011

    • Author(s)
      Kataoka, N., Diem, M. D., Yoshida, M., Hatai, C., Dobashi, I., Dreyfuss, G., Hagiwara, M. and Ohno, M
    • Journal Title

      Sci. Rep

      Volume: 1 Pages: 92

    • DOI

      DOI:10.1038/srep00092

    • Peer Reviewed
  • [Journal Article] Cajal body surveillance of U snRNA export complex assembly2010

    • Author(s)
      Suzuki, T., Izumi, H. and Ohno, M
    • Journal Title

      J. Cell Biol

      Volume: 190 Pages: 603-612

    • DOI

      doi:10.1083/jcb.201004109

    • Peer Reviewed
  • [Journal Article] A role for ubiquitin in the clearance of nonfunctional rRNAs2009

    • Author(s)
      Fujii, K., Sakata, T.,Kitabatake, M. and Ohno, M
    • Journal Title

      Genes Dev

      Volume: 23 Pages: 963-74

    • DOI

      doi:10.1101/gad.1775609

    • Peer Reviewed
  • [Journal Article] Functional association of the microprocessor complex with the spliceosome2009

    • Author(s)
      Kataoka, N., Fujita, M. and Ohno, M
    • Journal Title

      Mol. Cell. Biol

      Volume: 29 Pages: 3243-54

    • DOI

      doi:10.1128/MCB.00360-09

    • Peer Reviewed
  • [Journal Article] U1-independent pre-mRNA splicing contributes to the regulation of alternative Splicing2009

    • Author(s)
      Fukumura,K,. Taniguchi, I,. Sakamoto, H,. Ohno, M and Inoue,I
    • Journal Title

      Nucleic Acids Res

      Volume: Vol.37, No.6 Pages: 1907-1914

    • DOI

      doi:10.1093/nar/gkp050

    • Peer Reviewed
  • [Journal Article] Isolation and characterization of postsplicing lariat-intron complexes2009

    • Author(s)
      Yoshimoto, R., Kataoka, N., Okawa, K. and Ohno,M
    • Journal Title

      Nucleic Acids Res

      Volume: 37 Pages: 891-902

    • DOI

      doi:10.1093/nar/gkn1002

    • Peer Reviewed
  • [Journal Article] Immunoprecipitation analysis to study RNA-protein interactions in Xenopus oocytes2008

    • Author(s)
      Mabuchi, N., Masuyama, K. and Ohno, M
    • Journal Title

      Methods Mol. Biol

      Volume: 488 Pages: 257-65

    • DOI

      doi:10.1007/978-1-60327-475-3_18

    • Peer Reviewed
  • [Journal Article] ATP- dependent recruitment of export factor Aly/REF onto intronless mRNAs by RNA helicase UAP562008

    • Author(s)
      Taniguchi,I. and Ohno,M
    • Journal Title

      Mol. Cell. Biol

      Volume: 28 Pages: 601-8

    • DOI

      PMID:17984224

    • Peer Reviewed
  • [Journal Article] A compartmentalized phosphorylation/ dephosphorylation system that regulates U snRNA export from the nucleu2008

    • Author(s)
      Kitao, S., Segref, A., Kast, J., Wilm, M., Mattaj, I, W. and Ohno, M
    • Journal Title

      Mol. Cell. Biol

      Volume: 28 Pages: 487-97

    • DOI

      PMID:17967890

    • Peer Reviewed
  • [Presentation] Classical low-throughput analyses on RNA export from the nucleus2013

    • Author(s)
      Ohno,M
    • Organizer
      第2回 RNA and Biofunctions- ASIA Study
    • Place of Presentation
      Hotel Leopalace Hakata. Japan
    • Year and Date
      2013-01-10
  • [Presentation] Nuclear RNA Binding Protein hnRNP C Measures the Length of RNA Polymerase II Transcripts and Sorts them into two RNA Categories for Nuclear Export2012

    • Author(s)
      Ohno,M
    • Organizer
      第19回東アジアシンポジウム
    • Place of Presentation
      Hoam Faculty House. Seoul. South Korea
    • Year and Date
      20120822-24
  • [Presentation] 40S subunit dissociation and proteasome-dependent RNA degradation in nonfunctional 25S rRNA decay2012

    • Author(s)
      Kitabatake,M
    • Organizer
      The 22nd CDB Meeting
    • Place of Presentation
      RIKEN Center for Developmental biology. Kobe. Japan
    • Year and Date
      20120611-13
  • [Presentation] 40S subunit dissociation and proteasome-dependent RNA degradation in nonfunctional 25S rRNA decay2012

    • Author(s)
      Kitabatake,M
    • Organizer
      RNA 2012 Sixteenth Annual Meeting of the RNA Society
    • Place of Presentation
      University of Michigan. Detroit. USA
    • Year and Date
      20120329-0603
  • [Presentation] HnRNP C tetramer measures RNA length to classify RNA polymerase II transcripts for export2012

    • Author(s)
      McCloskey,A
    • Organizer
      The 10th International Student
    • Place of Presentation
      Shiran Kaikan, Inamori Hall. Kyoto. Japan
    • Year and Date
      20120329-0603
  • [Presentation] A novel activity of HIV-1 Rev protein to remodel export RNPs in directing virus-specific RNA export. Cerevisiae2011

    • Author(s)
      Taniguchi,I
    • Organizer
      2011 RNA meeting
    • Place of Presentation
      kyoto International Coference Cente. kyoto Japan
    • Year and Date
      20110614-19
  • [Presentation] In vivo reversal of a UV-induced lesion on mouse 28S rRNA2011

    • Author(s)
      Miyata,A
    • Organizer
      2011 RNA meeting
    • Place of Presentation
      kyoto International Coference Cente. kyoto Japan
    • Year and Date
      20110614-19
  • [Presentation] HnRNPC1/C2 tetramer measures RNA length to ensure the proper nuclear export of messenger RNAs2011

    • Author(s)
      McCloskey,A
    • Organizer
      2011 RNA meeting
    • Place of Presentation
      kyoto International Coference Cente. kyoto Japan
    • Year and Date
      20110614-19
  • [Presentation] Functional Decay of Nonfunctional 25S rRNA Requires Proteasome Activity2011

    • Author(s)
      Fujii,K
    • Organizer
      The 9th International Student Seminar.Kyoto
    • Place of Presentation
      Shiran Kaikan, Inamori Hall. kyoto Japan
    • Year and Date
      20110307-09
  • [Presentation] HnRNPC1/C2 tetramer measures RNA length prior to RNA export from the nucleus2010

    • Author(s)
      McCloskey,A
    • Organizer
      15th annual Meeting of the RNA Society
    • Place of Presentation
      University of Washington. Seattle USA
    • Year and Date
      20100622-26
  • [Presentation] A mechanism to measure RNA length prior to RNA export from the nucleus2010

    • Author(s)
      Ohno,M
    • Organizer
      19th CDB Meeting.May 10-12, 2010
    • Place of Presentation
      RIKEN Center for Developmental biology. kobe Japan
    • Year and Date
      20100510-12
  • [Presentation] Selective Degradation of Nonfunctional Ribosomal RNAs Mediated by the Ubiquitin-proteasome System2010

    • Author(s)
      Kitabatake,M
    • Organizer
      19th CDB Meeting
    • Place of Presentation
      RIKEN Center for Developmental biology. kobe Japan
    • Year and Date
      20100510-12
  • [Presentation] Analysis of cis- and trans- acting Elements for Nuclear Pre-mRNA Retention2010

    • Author(s)
      Takeiwa,T
    • Organizer
      The 8th International Student Seminar
    • Place of Presentation
      kyoto, Shiran Kaikan, Inamori Hall. kyoto Japan
    • Year and Date
      20100303-06
  • [Presentation] Functional Decay of Nonfunctional 25S rRNA Requires Proteasome Activity2009

    • Author(s)
      Fujii,K
    • Organizer
      The 16th East Asia Joint Conference on Biomedical Research
    • Place of Presentation
      Shiran Kaikai. Kyoto Japan
    • Year and Date
      20090913-14
  • [Presentation] A role for ubiquitin in the clearance of nonfunctional rRNAs2009

    • Author(s)
      Kitabatake,M
    • Organizer
      14th annual Meeting of the RNA Society
    • Place of Presentation
      University of Wisconsin campus. USA
    • Year and Date
      20090526-31
  • [Presentation] Regulations of nucleo- cytoplasmic RNA distribution2008

    • Author(s)
      Ohno,M
    • Organizer
      NAIST Global COE Symposium on Cell Signaling
    • Place of Presentation
      Nara Institute of Science and Technology. Nara Japan
    • Year and Date
      20081113-14

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Published: 2014-08-29  

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