Regulation of Nucleo-cytoplasmic Distribution of RNAs

2012 Fiscal Year Final Research Report

• Project Area: Diversity and asymmetry achieved by RNA program
• Project/Area Number: 20112007
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University
• Principal Investigator: OHNO Mutsuhito 京都大学, ウイルス研究所, 教授 (80201979)
• Project Period (FY): 2008 – 2012
• Keywords: RNA / 輸送 / 核 / 細胞質 / 識別

Research Abstract

We have shown that the tetramer of hnRNP C measures the length of the RNA polymerase II transcripts, like a molecular ruler, and classifies them into either mRNA or U snRNA according to their lengths. We have also revealed factors involved in the nuclear retention of pre-mRNAs in mammalian cells. Furthermore, we have obtained new insights in the area of RNA export.

Research Products

• [Journal Article] hDbr1 is a nucleocytoplasmic shuttling protein with a protein phosphatase-like motif essential for debranching activity (2013)
  • Author(s): Kataoka, N. Hagiwara, M. and Ohno, M
  • Journal Title: Sci. Rep Volume: 3-1090 Pages: 1090(オンライン番号)
  • DOI: doi:10.1038/srep01090
  • Peer Reviewed
• [Journal Article] Size matters in RNA export (2012)
  • Author(s): Ohno, M
  • Journal Title: RNA Biology Volume: 9(12) Pages: 1-5
  • DOI: doi:10.4161/rna.22569
  • Peer Reviewed
• [Journal Article] Mechanistic insights into human pre-mRNA splicing of human ultra-short introns: potential unusual mechanism identifies G-rich introns (2012)
  • Author(s): Sasaki-Haraguchi N, Shimada MK, Taniguchi I, Ohno M, Mayeda A
  • Journal Title: Biochem Biophys Res Commun Volume: 423(2) Pages: 289-94
  • DOI: doi:10.1016/j.bbrc.2012.05.112
  • Peer Reviewed
• [Journal Article] 40S subunit dissociation and proteasome-dependent RNA degradation in nonfunctional 25S rRNA decay (2012)
  • Author(s): Fujii,K., Sakata,T., Kitabatake,M. and Ohno, M
  • Journal Title: The EMBO Journa Volume: 31 Pages: 2579-2589
  • DOI: doi:10.1038/emboj.2012.85
  • Peer Reviewed
• [Journal Article] HnRNP C tetramer measures RNA length to classify RNA polymerase II transcripts for export (2012)
  • Author(s): McCloskey,A. Taniguchi,I,. Shinmyozu, K, and Ohno, M
  • Journal Title: Science Volume: 30 Pages: 1643-1646
  • Peer Reviewed
• [Journal Article] Multiple factors in the early splicing complex are involved in the nuclear retention of pre-mRNAs in mammalian cells (2011)
  • Author(s): Takemura,R., Takeiwa,T,. McCloskey, A.,Taniguchi,I. and Ohno, M
  • Journal Title: Genes Cells Volume: 16(10) Pages: 1035-49
  • DOI: DOI:10.1111/j.1365-2443.2011.01548
  • Peer Reviewed
• [Journal Article] Specific Y14 domains mediate its nucleo- cytoplasmic shuttling and association with spliced mRNA (2011)
  • Author(s): Kataoka, N., Diem, M. D., Yoshida, M., Hatai, C., Dobashi, I., Dreyfuss, G., Hagiwara, M. and Ohno, M
  • Journal Title: Sci. Rep Volume: 1 Pages: 92
  • DOI: DOI:10.1038/srep00092
  • Peer Reviewed
• [Journal Article] Cajal body surveillance of U snRNA export complex assembly (2010)
  • Author(s): Suzuki, T., Izumi, H. and Ohno, M
  • Journal Title: J. Cell Biol Volume: 190 Pages: 603-612
  • DOI: doi:10.1083/jcb.201004109
  • Peer Reviewed
• [Journal Article] A role for ubiquitin in the clearance of nonfunctional rRNAs (2009)
  • Author(s): Fujii, K., Sakata, T.,Kitabatake, M. and Ohno, M
  • Journal Title: Genes Dev Volume: 23 Pages: 963-74
  • DOI: doi:10.1101/gad.1775609
  • Peer Reviewed
• [Journal Article] Functional association of the microprocessor complex with the spliceosome (2009)
  • Author(s): Kataoka, N., Fujita, M. and Ohno, M
  • Journal Title: Mol. Cell. Biol Volume: 29 Pages: 3243-54
  • DOI: doi:10.1128/MCB.00360-09
  • Peer Reviewed
• [Journal Article] U1-independent pre-mRNA splicing contributes to the regulation of alternative Splicing (2009)
  • Author(s): Fukumura,K,. Taniguchi, I,. Sakamoto, H,. Ohno, M and Inoue,I
  • Journal Title: Nucleic Acids Res Volume: Vol.37, No.6 Pages: 1907-1914
  • DOI: doi:10.1093/nar/gkp050
  • Peer Reviewed
• [Journal Article] Isolation and characterization of postsplicing lariat-intron complexes (2009)
  • Author(s): Yoshimoto, R., Kataoka, N., Okawa, K. and Ohno,M
  • Journal Title: Nucleic Acids Res Volume: 37 Pages: 891-902
  • DOI: doi:10.1093/nar/gkn1002
  • Peer Reviewed
• [Journal Article] Immunoprecipitation analysis to study RNA-protein interactions in Xenopus oocytes (2008)
  • Author(s): Mabuchi, N., Masuyama, K. and Ohno, M
  • Journal Title: Methods Mol. Biol Volume: 488 Pages: 257-65
  • DOI: doi:10.1007/978-1-60327-475-3_18
  • Peer Reviewed
• [Journal Article] ATP- dependent recruitment of export factor Aly/REF onto intronless mRNAs by RNA helicase UAP56 (2008)
  • Author(s): Taniguchi,I. and Ohno,M
  • Journal Title: Mol. Cell. Biol Volume: 28 Pages: 601-8
  • DOI: PMID:17984224
  • Peer Reviewed
• [Journal Article] A compartmentalized phosphorylation/ dephosphorylation system that regulates U snRNA export from the nucleu (2008)
  • Author(s): Kitao, S., Segref, A., Kast, J., Wilm, M., Mattaj, I, W. and Ohno, M
  • Journal Title: Mol. Cell. Biol Volume: 28 Pages: 487-97
  • DOI: PMID:17967890
  • Peer Reviewed
• [Presentation] Classical low-throughput analyses on RNA export from the nucleus (2013)
  • Author(s): Ohno,M
  • Organizer: 第2回 RNA and Biofunctions- ASIA Study
  • Place of Presentation: Hotel Leopalace Hakata. Japan
  • Year and Date: 2013-01-10
• [Presentation] Nuclear RNA Binding Protein hnRNP C Measures the Length of RNA Polymerase II Transcripts and Sorts them into two RNA Categories for Nuclear Export (2012)
  • Author(s): Ohno,M
  • Organizer: 第19回東アジアシンポジウム
  • Place of Presentation: Hoam Faculty House. Seoul. South Korea
  • Year and Date: 20120822-24
• [Presentation] 40S subunit dissociation and proteasome-dependent RNA degradation in nonfunctional 25S rRNA decay (2012)
  • Author(s): Kitabatake,M
  • Organizer: The 22nd CDB Meeting
  • Place of Presentation: RIKEN Center for Developmental biology. Kobe. Japan
  • Year and Date: 20120611-13
• [Presentation] 40S subunit dissociation and proteasome-dependent RNA degradation in nonfunctional 25S rRNA decay (2012)
  • Author(s): Kitabatake,M
  • Organizer: RNA 2012 Sixteenth Annual Meeting of the RNA Society
  • Place of Presentation: University of Michigan. Detroit. USA
  • Year and Date: 20120329-0603
• [Presentation] HnRNP C tetramer measures RNA length to classify RNA polymerase II transcripts for export (2012)
  • Author(s): McCloskey,A
  • Organizer: The 10th International Student
  • Place of Presentation: Shiran Kaikan, Inamori Hall. Kyoto. Japan
  • Year and Date: 20120329-0603
• [Presentation] A novel activity of HIV-1 Rev protein to remodel export RNPs in directing virus-specific RNA export. Cerevisiae (2011)
  • Author(s): Taniguchi,I
  • Organizer: 2011 RNA meeting
  • Place of Presentation: kyoto International Coference Cente. kyoto Japan
  • Year and Date: 20110614-19
• [Presentation] In vivo reversal of a UV-induced lesion on mouse 28S rRNA (2011)
  • Author(s): Miyata,A
  • Organizer: 2011 RNA meeting
  • Place of Presentation: kyoto International Coference Cente. kyoto Japan
  • Year and Date: 20110614-19
• [Presentation] HnRNPC1/C2 tetramer measures RNA length to ensure the proper nuclear export of messenger RNAs (2011)
  • Author(s): McCloskey,A
  • Organizer: 2011 RNA meeting
  • Place of Presentation: kyoto International Coference Cente. kyoto Japan
  • Year and Date: 20110614-19
• [Presentation] Functional Decay of Nonfunctional 25S rRNA Requires Proteasome Activity (2011)
  • Author(s): Fujii,K
  • Organizer: The 9th International Student Seminar.Kyoto
  • Place of Presentation: Shiran Kaikan, Inamori Hall. kyoto Japan
  • Year and Date: 20110307-09
• [Presentation] HnRNPC1/C2 tetramer measures RNA length prior to RNA export from the nucleus (2010)
  • Author(s): McCloskey,A
  • Organizer: 15th annual Meeting of the RNA Society
  • Place of Presentation: University of Washington. Seattle USA
  • Year and Date: 20100622-26
• [Presentation] A mechanism to measure RNA length prior to RNA export from the nucleus (2010)
  • Author(s): Ohno,M
  • Organizer: 19th CDB Meeting.May 10-12, 2010
  • Place of Presentation: RIKEN Center for Developmental biology. kobe Japan
  • Year and Date: 20100510-12
• [Presentation] Selective Degradation of Nonfunctional Ribosomal RNAs Mediated by the Ubiquitin-proteasome System (2010)
  • Author(s): Kitabatake,M
  • Organizer: 19th CDB Meeting
  • Place of Presentation: RIKEN Center for Developmental biology. kobe Japan
  • Year and Date: 20100510-12
• [Presentation] Analysis of cis- and trans- acting Elements for Nuclear Pre-mRNA Retention (2010)
  • Author(s): Takeiwa,T
  • Organizer: The 8th International Student Seminar
  • Place of Presentation: kyoto, Shiran Kaikan, Inamori Hall. kyoto Japan
  • Year and Date: 20100303-06
• [Presentation] Functional Decay of Nonfunctional 25S rRNA Requires Proteasome Activity (2009)
  • Author(s): Fujii,K
  • Organizer: The 16th East Asia Joint Conference on Biomedical Research
  • Place of Presentation: Shiran Kaikai. Kyoto Japan
  • Year and Date: 20090913-14
• [Presentation] A role for ubiquitin in the clearance of nonfunctional rRNAs (2009)
  • Author(s): Kitabatake,M
  • Organizer: 14th annual Meeting of the RNA Society
  • Place of Presentation: University of Wisconsin campus. USA
  • Year and Date: 20090526-31
• [Presentation] Regulations of nucleo- cytoplasmic RNA distribution (2008)
  • Author(s): Ohno,M
  • Organizer: NAIST Global COE Symposium on Cell Signaling
  • Place of Presentation: Nara Institute of Science and Technology. Nara Japan
  • Year and Date: 20081113-14