Study on role of intracellular degradative logistics in diseases and its molecular machinery

2012 Fiscal Year Final Research Report

• Project Area: Intracellular logistics: interdisciplinary approaches to pathophysiology of membrane traffic
• Project/Area Number: 20113002
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Osaka University
• Principal Investigator: YOSHIMORI Tamotsu 大阪大学, 大学院・生命機能研究科, 教授 (60191649)
• Project Period (FY): 2008 – 2012
• Keywords: オートファジー / エンドサイトーシス経路

Research Abstract

Both the endocytic and autophagic pathway delivers materials into lysosomes, intracellular digestive organelles, from outside of cells and from the cytoplasm, respectively. The pathways tune when, how much, and what is degraded in lysosomes. We revealed molecular machineries underlying the pathways and their role in diseases including infectious diseases, cancer, and osteopetrosis.

Research Products

• [Journal Article] Autophagy Plays a Significant Role in Determining Skin Color by Regulating Melanosome Degradation in Keratinocytes (2013)
  • Author(s): Murase D, Hachiya A, Takano K, Hicks R, Visscher MO, Kitahara T, Hase T, Takema Y, Yoshimori T
  • Journal Title: J Invest Dermatol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Impaired autophagy by soluble endoglin, under physiological hypoxia in early pregnant period, is involved in poor placentation in preeclampsia (2013)
  • Author(s): Nakashima A, Yamanaka-Tatematsu M, Fujita N, Koizumi K, Shima T, Yoshida T, Nikaido T, Okamoto A, Yoshimori T, Saito S
  • Journal Title: Autophagy Volume: 9 Pages: 303-316
  • Peer Reviewed
• [Journal Article] A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery (2011)
  • Author(s): Katayama H, Kogure T, Mizushima N, Yoshimori T, Miyawaki A
  • Journal Title: Chem Biol Volume: 18 Pages: 1042-1052
  • Peer Reviewed
• [Journal Article] The LC3 recruitment mechanism is separate from Atg9L1-dependent membrane formation in the autophagic response against Salmonella (2011)
  • Author(s): Kageyama S, Omori H, Saitoh T, Sone T, Guan JL, Akira S, Imamoto F, Noda T, Yoshimori T
  • Journal Title: Mol Biol Cell Volume: 22 Pages: 2290-2300
  • Peer Reviewed
• [Journal Article] Rubicon and PLEKHM1 Negatively Regulate the Endocytic/Autophagic Pathway via a Novel Rab7-binding Domain (2010)
  • Author(s): Tabata K, Matsunaga K, Sakane A, Sasaki T, Noda T, Yoshimori T
  • Journal Title: Mol Biol Cell Volume: 21 Pages: 4162-4172
  • Peer Reviewed
• [Journal Article] Autophagy requires endoplasmic reticulum targeting of the PI3-kinase complex via Atg14L (2010)
  • Author(s): Matsunaga K, Morita E, Saitoh T, Akira S, Ktistakis NT, Izumi T, Noda T, Yoshimori T
  • Journal Title: J Cell Biol Volume: 190 Pages: 511-521
  • Peer Reviewed
• [Journal Article] Combinational soluble N-ethylmaleimide-sensitive factor attachment protein receptor proteins VAMP8 and Vti1b mediate fusion of antimicrobial and canonical autophagosomes with lysosomes (2010)
  • Author(s): Furuta N, Fujita N, Noda T, Yoshimori T, Amano A
  • Journal Title: Mol Biol Cell Volume: 21 Pages: 1001-1010
  • Peer Reviewed
• [Journal Article] Modulation of Local PtdIns3P Levels by the PI Phosphatase MTMR3 Regulates Constitutive Autophagy (2010)
  • Author(s): Taguchi-Atarashi N, Hamasaki M, Matsunaga K, Omori H, Ktistakis NT, Yoshimori T, Noda T
  • Journal Title: Traffic Volume: 11 Pages: 468-478
  • Peer Reviewed
• [Journal Article] An initial step of GAS-containing autophagosome-like vacuoles formation requires Rab7 (2009)
  • Author(s): Yamaguchi H, Nakagawa I, Yamamoto A, Amano A, Noda T, Yoshimori T
  • Journal Title: PLoS Pathog Volume: 5 Pages: e1000670
  • Peer Reviewed
• [Journal Article] Atg9a controls dsDNA-driven dynamic translocation of STING and the innate immune response (2009)
  • Author(s): Saitoh T§, Fujita N§, Hayashi T, Takahara K, Satoh T, Lee H, Matsunaga K, Kageyama S, Omori H, Noda T, Yamamoto N, Kawai T, Ishii K, Takeuchi O, Yoshimori T, Akira S
  • Journal Title: Proc Natl Acad Sci USA Volume: 106 Pages: 20842-20846
  • Peer Reviewed
• [Journal Article] A subdomain of the endoplasmic reticulum forms a cradle for autophagosome formation (2009)
  • Author(s): Hayashi-Nishino M, Fujita N, Noda T, Yamaguchi A, Yoshimori T, Yamamoto A
  • Journal Title: Nat Cell Biol Volume: 11 Pages: 1433-1437
  • Peer Reviewed
• [Journal Article] Differencial involvement of ATG16L1 in Crohn's disease and canonical autophagy: analysis of the organization of the ATG16L1 complex in fibroblasts (2009)
  • Author(s): Fujita N, Saitoh T, Kageyama S, Akira S, Noda T, Yoshimori T
  • Journal Title: J Biol Chem Volume: 284 Pages: 32602-32609
  • Peer Reviewed
• [Journal Article] Two Beclin-1 binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages (2009)
  • Author(s): Matsunaga K, Saitoh T, Tabata K, Omori H, Satoh T, Kurotori N, Maejima I, Shirahama-Noda, K, Ichimura I, Isobe T, Akira S, Noda T, Yoshimori T
  • Journal Title: Nature Cell Biol Volume: 11 Pages: 385-396
  • Peer Reviewed
• [Journal Article] Loss of the autophagy protein Atg16L1 enhances endotoxin-induced IL-1beta production (2008)
  • Author(s): Saitoh T§, Fujita N§, Jang MH, Uematsu S, Yang BG, Satoh T, Omori H, Noda T, Yamamoto N, Komatsu M, Tanaka K, Kawai T, Tsujimura T, Takeuchi O, Yoshimori T, Akira S
  • Journal Title: Nature Volume: 456 Pages: 264-268
  • Peer Reviewed
• [Journal Article] DAP-kinase is a mediator of endoplasmic reticulum stress-induced caspase activation and autophagic cell death (2008)
  • Author(s): Gozuacik D, Bialik S, Raveh T, Mitou G, Shohat G, Sabanay H, Mizushima N, Yoshimori T, Kimchi A
  • Journal Title: Cell Death Differ Volume: 15 Pages: 1875-1886
  • Peer Reviewed
• [Journal Article] An Atg4B Mutant Hampers the Lipidation of LC3 Paralogues and Causes Defects in Autophagosome Closure (2008)
  • Author(s): Fujita N, Hayashi M, Fukumoto H, Omori H, Yamamoto A, Noda T, Yoshimori T
  • Journal Title: Mol Biol Cell Volume: 19 Pages: 4651-4659
  • Peer Reviewed
• [Journal Article] Autophagic control of listeria through intracellular innate immune recognition in drosophila (2008)
  • Author(s): Yano T, Mita S, Ohmori H, Oshima Y, Fujimoto Y, Ueda R, Takada H, Goldman WE, Fukase K, Silverman N, Yoshimori T, Kurata S
  • Journal Title: Nat Immunol Volume: 9 Pages: 908-916
  • Peer Reviewed
• [Presentation] Mechanistic Insights into Autophagy against Bacteria Invading Host Cells (2012)
  • Author(s): Tamotsu Yoshimori
  • Organizer: Cambridge Immunology Forum 2012, (Roche Distinguished Lecturer)
  • Place of Presentation: Cambridge, UK
  • Year and Date: 2012-09-20
• [Presentation] Insights into Membrane Dynamics in Autophagy (2011)
  • Author(s): Tamotsu Yoshimori
  • Organizer: EMBO Conference on Autophagy in Health and Disease in Tel Aviv
  • Place of Presentation: Israel
  • Year and Date: 2011-10-31
• [Presentation] Mechanistic Insight into Membrane Dynamics in Mammalian Autophagy (2011)
  • Author(s): Tamotsu Yoshimori
  • Organizer: Keystone Symposia on Molecular and Cellular Biology "Autophagy (D1)"
  • Place of Presentation: Whistler, Canada
  • Year and Date: 2011-03-28
• [Presentation] Mechanistic insight into membrane dynamics of mammalian autophagy (2010)
  • Author(s): Tamotsu Yoshimori
  • Organizer: Gordon Research Conference "Autophagy In Stress, Development And Disease" in Lucca
  • Place of Presentation: Italy
  • Year and Date: 2010-04-27
• [Presentation] Binding Rubicon to Cross the Rubicon (2009)
  • Author(s): Tamotsu Yoshimori
  • Organizer: EMBO Conference Series on Autophagy: Cell Biology, Physiology & Pathology in Ascona
  • Place of Presentation: Switzerland
  • Year and Date: 2009-10-19
• [Presentation] Mechanistic Insight into Membrane Dynamics in Mammalian Autophagy (2009)
  • Author(s): Tamotsu Yoshimori
  • Organizer: Gordon Research Conference "Molecular Membrane Biology" in Andover
  • Place of Presentation: NH, USA
  • Year and Date: 2009-07-09
• [Book] Q&A / 哺乳類におけるオートファジー関連因子オートファジー生命をささえる細胞の自己分解システム 水島昇・吉森保 編 (2012)
  • Author(s): 吉森 保
  • Total Pages: 48-66
  • Publisher: 化学同人 C1-10
• [Book] Group a streptococcus: a loser in the battle with autophagy. Beth Levine・Tamotsu Yoshimori・Vojo Deretic Editors, Curr Top Microbiol Immunol. 335 (2009)
  • Author(s): Yoshimori T, Amano A
  • Total Pages: 217-226
  • Publisher: Springer
• [Book] Monitoring autophagy in mammalian cultured cells through the dynamics of LC3. Methods Enzymol. 452 (2009)
  • Author(s): Kimura S, Fujita N, Noda T, Yoshimori T
  • Total Pages: 1-12
  • Publisher: Academic Press,Inc.
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/yoshimori/jp/achievement/010/