2012 Fiscal Year Final Research Report
Molecular mechanism of regulated exocytosis and its involvement in endocrine and metabolic diseases
| Project Area | Intracellular logistics: interdisciplinary approaches to pathophysiology of membrane traffic |
|---|
| Project/Area Number |
20113005
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
|
| Allocation Type | Single-year Grants |
|---|
| Review Section |
Biological Sciences
|
|---|
| Research Institution | Gunma University |
|---|
Principal Investigator |
IZUMI Tetsuro 群馬大学, 生体調節研究所, 教授 (00212952)
|
|---|
| Project Period (FY) |
2008 – 2012
|
| Keywords | 分泌穎粒 / 調節性分泌 / 開口放出 / 糖尿病 / 内分泌 / Rabタンパク質 |
|---|
| Research Abstract |
We aimed to identify molecular mechanism and functional significance of granule docking to the plasma membrane in regulated exocytosis. For this purpose, we first analyzed the function of the Rab27 effector granuphilin and its partner, syntaxin-la, using gene-knockout mice. We found that only granuphilin, but not syntaxin-la, is essential for the docking process. We also established the microscopic system to simultaneously observe behavior of granules and associated molecules in living cells to explore the relationship between granule docking and fusion. Furthermore, we discovered the roles of other Rab27 effectors in regulated exocytosis: exiphilin7 functions in the exocytosis of undocked granules, whereas exophilin8 is involved in the recruitment of granules into the cortical actin network.
|
-
-
-
-
-
-
-
-
-
-
[Journal Article] Involvement of Vps33a in the fusion of uroplakin-degrading multivesicular bodies with lysosomes2009
Author(s)
Guo X, Tu L, Gumper I, Plesken H, Novak EK, Chintala S, Swank RT, Pastores G, Torres P, Izumi T, Sun T-T, Sabatini DD, and Kreibich G
-
Journal Title
Traffic
Volume: 10
Pages: 1350-136
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
