Functional Organization of the Genome

2012 Fiscal Year Final Research Report

• Project Area: The physicochemical field for genetic activities
• Project/Area Number: 20114002
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Osaka University
• Principal Investigator: HIRAOKA Yasushi 大阪大学, 生命機能研究科, 教授 (10359078)
• Co-Investigator(Kenkyū-buntansha): CHIKASHIGE Yuji (独)情報通信研究機構, 未来ICT研究所, 主任研究員 (60359081)
• Project Period (FY): 2008 – 2012
• Keywords: 分子細胞生物学

Research Abstract

In order study nuclear structures that ensure correct transmission of chromosomes to offspring, we developed a library of fluorescent proteins, DNA microarray and live-cell imaging technologies. Using these tools, we examined nuclear structures during meiosis in fission yeast and have revealed molecular mechanisms for transmission of chromosomes. We also examined nuclear structures in human cells, and obtained new insights into mechanisms how the nuclear envelope affects genetic activities of chromosomes.

Research Products

• [Journal Article] Purification and characterization of the fission yeast telomere clustering factors, Bqt1 and Bqt2 (2013)
  • Author(s): Ichikawa Y, Kagawa W, Saito K, Chikashige Y, Haraguchi T, Hiraoka Y, Kurumizaka H
  • Journal Title: Protein Expr. Purif Volume: 88 Pages: 207-213
  • DOI: doi:10.1016/j.pep.2013.01.006
  • Peer Reviewed
• [Journal Article] Autophagosomes form at ER-mitochondria contact sites (2013)
  • Author(s): Hamasaki M, Furuta N, Matsuda A, Nezu A, Yamamoto A, Fujita N, Oomori H, Noda T, Haraguchi T, Hiraoka Y, Yoshimori T, Amano A
  • Journal Title: Nature Volume: 495 Pages: 389-393
  • Peer Reviewed
• [Journal Article] Meiosis-specific non-coding RNA mediates robust pairing of homologous chromosomes in meiosis (2012)
  • Author(s): Ding DQ, Okamasa K, Yamane M, Tsutsumi C, Haraguchi T, Yamamoto M, and Hiraoka Y
  • Journal Title: Science Volume: 336 Pages: 732-736
  • DOI: doi:10.1126/science.1219518
  • Peer Reviewed
• [Journal Article] Telomere-Nuclear Envelope Dissociation Promoted by Rap1 Phosphorylation Ensures Faithful Chromosome Segregation (2012)
  • Author(s): Fujita I, Nishihara Y, Tanaka M, Tsujii H, Chikashige Y, Watanabe Y, Saito M, Ishikawa F, Hiraoka Y, Kanoh J
  • Journal Title: Curr. Biol Volume: 22 Pages: 1932-1937
  • DOI: doi:10.1016/j.cub.2012.08.019
  • Peer Reviewed
• [Journal Article] Lamin B receptor recognizes specific modification of histone H4 in heterochromatin formation (2012)
  • Author(s): Hirano Y, Hizume K, Kimura H, Takeyasu K, Haraguchi T, Hiraoka Y
  • Journal Title: J. Biol. Chem Volume: 287 Pages: 42654-42663
  • DOI: doi:10.1074/jbc.M112.397950
  • Peer Reviewed
• [Journal Article] Physical breakdown of the nuclear envelope is not necessary for breaking its barrier function (2011)
  • Author(s): Asakawa H, Hiraoka Y, Haraguchi T
  • Journal Title: Nucleus Volume: 2 Pages: 523-526
  • DOI: doi:10.4161/nucl. 2.6.16117
  • Peer Reviewed
• [Journal Article] Exportin 4 interacts with Sox9 through the HMG box and inhibits the DNA binding of Sox9 (2011)
  • Author(s): Tsuchiya M, Ogawa H, Suzuki T, Sugiyama N, Haraguchi T Hiraoka Y
  • Journal Title: PLoS ONE Volume: 6 Pages: e25694
  • DOI: doi:10.1371/journal.pone.0025694
  • Peer Reviewed
• [Journal Article] Inner nuclear membrane protein Ima1 is dispensable for intranuclear positioning of centromeres (2011)
  • Author(s): Hiraoka Y, Maekawa H, Asakawa H, Chikashige Y, Kojidani T, Osakada H, Matsuda A, Haraguchi T
  • Journal Title: Genes Cells Volume: 16 Pages: 1000-1011
  • DOI: doi:10.1111/j.365-2443.2011.01544.x
  • Peer Reviewed
• [Journal Article] Aneuploidy drives genomic instability in yeast (2011)
  • Author(s): Sheltzer JM, Blank HM, Pfau SJ, Tange Y, George BM, Humpton TJ, BritoIL, Hiraoka Y, Niwa O, Amon A
  • Journal Title: Science Volume: 333 Pages: 1026-30
  • DOI: doi:10.1126/science.1206412
  • Peer Reviewed
• [Journal Article] A conserved motif within RAP1 has diversified roles in telomere protection and regulation in different organisms (2011)
  • Author(s): Chen Y, Rai R, Zhou ZR, Kanoh J, Ribeyre C, Yang Y, Zheng H, Damay P, Wang F, Tsujii H, Hiraoka Y, Shore D, Hu HY, Chang S, Lei M
  • Journal Title: Nat. Struct. Mol. Biol Volume: 18 Pages: 213-21
  • DOI: doi:10.1038/nsmb.1974
  • Peer Reviewed
• [Journal Article] Virtual breakdown of the nuclear envelope in fission yeast meiosis (2010)
  • Author(s): Asakawa H, Kojidani T, Mori C, Osakada H, Sato M, Ding DQ, Hiraoka Y, Haraguchi T
  • Journal Title: Curr. Biol Volume: 20 Pages: 1919-1925
  • DOI: doi:10.1016/j.cub.2010. 09.070
  • Peer Reviewed
• [Journal Article] Nuclear envelope attachment is not necessary for telomere function in fission yeast (2010)
  • Author(s): Chikashige Y, Haraguchi T, Hiraoka Y
  • Journal Title: Nucleus Volume: 1 Pages: 481-486
  • DOI: doi:10.4161/nucl.1.6.13113
  • Peer Reviewed
• [Journal Article] In vivo evidence for the fibrillar structures of Sup35 prions in yeast cells (2010)
  • Author(s): Kawai-Noma S, Pack C-G, Kojidani T, Asakawa H, Hiraoka Y, Kinjo M, Haraguchi T, Taguchi H, Hirata A
  • Journal Title: J. Cell Biol Volume: 190 Pages: 223-231
  • Peer Reviewed
• [Journal Article] Membrane proteins Bqt3 and Bqt4 anchor telomeres to the nuclear envelope to ensure chromosomal bouquet formation (2009)
  • Author(s): Chikashige Y, Yamane M, Okamasa K, Tsutsumi C, Kojidani T, Sato M, Haraguchi T, Hiraoka Y
  • Journal Title: J. Cell Biol Volume: 187 Pages: 413-427
  • DOI: doi:10.1083/jcb.200902122
  • Peer Reviewed
• [Journal Article] The SUN rises on meiotic chromosome dynamics (2009)
  • Author(s): Hiraoka Y, Dernburg AF
  • Journal Title: Dev. Cell Volume: 17 Pages: 598-605
  • Peer Reviewed
• [Journal Article] Transcriptional Suppression by Transient Recruitment of ARIP4 to Sumoylated Nuclear Receptor Ad4BP/SF-1 (2009)
  • Author(s): Ogawa H, Komatsu K, Hiraoka Y, and Morohashi K
  • Journal Title: Mol. Biol. Cell Volume: 20 Pages: 4235-4245
  • DOI: doi:10.1091/mbc.E08-12-1247
  • Peer Reviewed
• [Journal Article] Localization of gene products using a chromosomally tagged GFP-fusion library in the fission yeast Schizosaccharomyces pombe (2009)
  • Author(s): Hayashi A, Ding, DQ, Tsutsumi C, Chikashige Y, Masuda H, Haraguchi T and Hiraoka Y
  • Journal Title: Genes Cells Volume: 14 Pages: 217-225
  • DOI: doi:10.1111/j.1365-2443.2008.01264.x
  • Peer Reviewed
• [Presentation] Live correlative light and electron microscopy of mammalian and yeast cells (2012)
  • Author(s): Hiraoka Y, Haraguchi T
  • Organizer: ICHC Kyoto2012 (国際組織細胞化学会) 14th International Congress of Histochemistry and Cytochemistry
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 2012-08-27
• [Presentation] The Nuclear Envelope in Meiosis (2012)
  • Author(s): Hiraoka Y
  • Organizer: The 20th Jerusalem School in Life Sciences "Nuclear Organization, Dynamics and Activity"
  • Place of Presentation: Israel Institute for Advanced Studies at the Hebrew University, Israel
  • Year and Date: 2012-07-23
• [Presentation] Meiosis-specific non-coding RNA in homologous chromosome pairing (2012)
  • Author(s): Hiraoka Y
  • Organizer: Gordon Research Conference "Meiosis"
  • Place of Presentation: Colby-Sawyer College, USA
  • Year and Date: 2012-06-06
• [Presentation] Meiosis-specific non-coding RNA mediates pairing of homologous chromosomes in meiosis (2012)
  • Author(s): Hiroaka Y
  • Organizer: Gordon Research Conference "Chromatin Structure & Function"
  • Place of Presentation: Colby-Sawyer College, USA
  • Year and Date: 2012-05-09
• [Presentation] Ding D-Q, Haraguchi T. Meiosis-specific non-coding RNA mediates robust pairing of homologous chromosomes in meiosis (2011)
  • Author(s): Hiraoka Y
  • Organizer: The EMBO Conference on Meiosis (2^<nd>)
  • Place of Presentation: Capaccio/Paestum, Italy
  • Year and Date: 2011-09-19
• [Presentation] Meiosis-specific non-coding RNA mediates robust pairing of homologous chromosomes in meiosis (2011)
  • Author(s): Hiraoka Y
  • Organizer: Gordon Research Conference "Chromosome Dynamics"
  • Place of Presentation: Mount Snow Resort, USA
  • Year and Date: 2011-07-13
• [Presentation] Pairing and recognition of homologous chromosomes in meiosis (2011)
  • Author(s): Hiraoka Y, Ding D-Q, Haraguchi T
  • Organizer: Pombe2011 The 6th International Fission Yeast Meeting
  • Place of Presentation: Boston, U.S.A
  • Year and Date: 2011-06-29
• [Presentation] Nuclear membrane properties in mitosis and meiosis (2010)
  • Author(s): Hiraoka Y
  • Organizer: Gordon Research Conferences "Meiosis"
  • Place of Presentation: Colby-Sawyer College New London, USA
  • Year and Date: 2010-06-16
• [Presentation] Large-scale localization of protein components of nuclear structures in fission yeast (2009)
  • Author(s): Hiraoka Y, Chikashige Y, Ding D-Q, Asakawa H, Maekawa H, Haraguchi T
  • Organizer: The 5th International Fission Yeast Meeting
  • Place of Presentation: National Olympics Memorial Youth Center, Tokyo, Japan
  • Year and Date: 2009-10-30
• [Book] 遺伝情報の時空間場の理解に向けて. 実験医学 増刊 細胞核-遺伝情報制御と疾患 (2009)
  • Author(s): 平岡 泰
  • Total Pages: 27,2708-2714
  • Publisher: 羊土社
• [Book] Live CLEM imaging: an application for yeast cells In "Current microscopy contributions to advances in science and technology", vol.1
  • Author(s): Asakawa H, Hiraoka Y, Haraguchi T
  • Total Pages: 478-485
  • Publisher: A. Mendez-Vilas (Ed.) Formatex Research Center
• [Remarks]
  • URL: http://www.genofield.osaka-u.ac.jp/pfga_index.html
• [Patent(Industrial Property Rights)] Vrk1蛋白質抗体とその製造方法、抗原、検知方法 (2009)
  • Inventor(s): 原口徳子、荒神尚子、平岡泰
  • Industrial Property Rights Holder: 独立行政法人 情報通信研究機構
  • Industrial Property Number: 特許権、特願2009-155028
  • Filing Date: 2009-06-30