Dynamics of the genome compaction in a nucleus

2010 Fiscal Year Self-evaluation Report

• Project Area: The physicochemical field for genetic activities
• Project/Area Number: 20114003
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Waseda University
• Principal Investigator: OHYAMA Takashi Waseda University, 教育・総合科学学術院, 教授 (60268513)
• Project Period (FY): 2008-11-13 – 2013-03-31
• Keywords: ゲノム / 染色体 / 細胞核 / クロマチン / DNA物性 / DNA高次構造 / 遺伝子発現 / 自己集合

Research Abstract

本研究では、以下の目標を掲げた。
(1)DNAの物理的性質を糸口にしてクロマチン・染色体の構築機構にメスをいれる。(2)生細胞蛍光イメージングの手法を用いて、DNA物性特異領域の核内(または染色体内)空間配置を可視化し、細胞周期および細胞分化に伴うゲノム折り畳みのダイナミクスを明らかにする。(3)酵母、線虫、ショウジョウバエ、メダカ、マウス、チンパンジーなどのゲノムに関しても機械的特性の解析を行うと共に、一部についてはゲノム折り畳みのダイナミクスを解析する。そして、これらの各研究成果を総合的に分析して、真核生物に共通したゲノム収納の分子基盤を解明する。

Research Products

• [Journal Article] Competence of an artificial bent DNA as a transcriptional activator in mouse ES cells. (2011)
  • Author(s): Tanase, J., Mitani, T., Udagawa, K., Nishikawa, J., Ohyama, T.
  • Journal Title: Mol.Biol.Rep. 38 Pages: 37-47
  • Peer Reviewed
• [Journal Article] Highly efficient chromatin transcription induced by superhelically curved DNA segments : the underlying mechanism revealed by a yeast system. (2010)
  • Author(s): Tanase, J., Morohashi, N., Fujita, M., Nishikawa, J., Shimizu, M., Ohyama, T.
  • Journal Title: Biochemistry 49 Pages: 2351-2358
  • Peer Reviewed
• [Journal Article] Effects of carriers on transgene expression from plasmids containing a DNA sequence with high histone affinity. (2009)
  • Author(s): Kamiya, H., Fukunaga, S., Ohyama, T., Harashima, H.
  • Journal Title: Int.J.Pharm. 376 Pages: 99-103
  • Peer Reviewed
• [Journal Article] The human actin-related protein hArp5 : nucleo-cytoplasmic shuttling and involvement in DNA repair. (2009)
  • Author(s): Kitayama, K., Kamo, M., Oma, Y., Matsuda, R., Uchida, T., Ikura, T., Tashiro, S., Ohyama, T., Winsor, B., Harata, M.
  • Journal Title: Exp.Cell Res. 315 Pages: 206-217
  • Peer Reviewed
• [Journal Article] ゲノムDNAはいかにして折り畳まれるか-DNA物性とゲノム収納- (2009)
  • Author(s): 大山隆, 木村元, 下岡保俊
  • Journal Title: 実験医学増刊号「細胞核-遺伝情報制御と疾患」 27 Pages: 35-42
• [Presentation] Structure of yeast interphase chromosomes and the genome folding principle. (2011)
  • Author(s): Ohyama T., 他8名
  • Organizer: International Symposium on the Physicochemical Field for Genetic Activities
  • Place of Presentation: Awaji
  • Year and Date: 2011-06-24
• [Presentation] Three-dimensional architecture of chromatin fibers in the interphase nucleus. (2010)
  • Author(s): Kimura H., 中4名, Ohyama T.
  • Organizer: The American Society for Cell Biology 50th Annual Meeting
  • Place of Presentation: Philadelphia
  • Year and Date: 2010-12-13
• [Presentation] How genomic DNA is functionally folded in a nucleus. (2009)
  • Author(s): Ohyama T., 他6名
  • Organizer: The Wilhelm Bernhard Workshop 21st International Workshop on the Cell Nucleus
  • Place of Presentation: Ustron, Poland
  • Year and Date: 2009-09-01
• [Presentation] How genomic DNA is functionally folded in a nucleus. (2009)
  • Author(s): Ohyama T., 他6名
  • Organizer: 18th Lake Shirakaba Conference
  • Place of Presentation: Copenhagen
  • Year and Date: 2009-06-20
• [Presentation] The DNA flexibility maps of eukaryotic genomes. (2008)
  • Author(s): Ohyama, T., 他3名.
  • Organizer: 8th EMBL Transcription Meeting
  • Place of Presentation: Heidelberg
  • Year and Date: 2008-08-23
• [Book] ベーシックマスター生化学 (2008)
  • Author(s): 大山隆監修, 西川一八・清水光弘共編
  • Total Pages: 427
  • Publisher: オーム社