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2013 Fiscal Year Final Research Report

Elucidation of molecular basis of the field for gene expression by proteomics

Planned Research

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Project AreaThe physicochemical field for genetic activities
Project/Area Number 20114006
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionHokkaido University

Principal Investigator

OBUSE Chikashi  北海道大学, 先端生命科学研究科(研究院), 教授 (00273855)

Co-Investigator(Kenkyū-buntansha) NAGAO Koji  北海道大学, 大学院先端生命科学研究院, 講師 (60426575)
Project Period (FY) 2008-11-13 – 2013-03-31
Keywords染色体 / ヘテロクロマチン / エピジェネティクス / 非コードRNA / ヒストン / HP1 / Aurora B
Research Abstract

Heterochromatin Protein 1 (HP1) is thought to play a role in compacted heterochromatin formation, through binding to K9 trimethylated histone H3 and its interacting proteins. By proteomic analysis, we identified 82 HP1 binding proteins (HPBPs) in human cells. In addition to known HPBPs, several uncharacterized and/or unexpected proteins were identified [Nature Cell Biol. 12: 719, 2010]. Interestingly, an identified HP1 binding protein was enriched in inactive X chromosomes (Xi), in association with SMCHD1, and thus we named it HBiX1 (HP1 binding protein enriched in Xi). Cytological and epigenomic analyses revealed that HBiX1 and SMCHD1 mediate the compaction of Xi to form heterochromatin structure, by linking the H3K9me3 domains and the XIST/H3K27me3 domains [Nature Struct. & Mol. Biol. 20: 566, 2013]. These studies uncovered that new molecular insights of heterochromatin, especially, how epigenetic marks are translated into higher order chromatin structure to repress transcription.

  • Research Products

    (47 results)

All 2013 2012 2011 2010 2009 2008 Other

All Journal Article (19 results) (of which Peer Reviewed: 19 results) Presentation (16 results) Book (7 results) Remarks (5 results)

  • [Journal Article] Human origin recognition complex binds preferentially to -quadruplex-preferable RNA and single-stranded DNA2013

    • Author(s)
      Hoshina S.、Yura K.、Teranishi H.、Kiyasu N.、Tominaga A.、Kadoma H.、Nakatsuka A.、 Kunichika T.、Obuse C.、Waga S.
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 288(42) Pages: 30161-30171

    • DOI

      10.174/jbc.M113.492504

    • Peer Reviewed
  • [Journal Article] Genetically encoded system to track histone modification in vivo2013

    • Author(s)
      Sato Y.、Mukai M.、Ueda J.、Muraki M.、 Stasevich T.J.、Horikoshi N.、Kujirai T.、 Kita H.、Kimura T.、Hira S.、Okada Y.、 Hayashi-Takanaka Y.、Obuse C.、 Kurumizaka H.、Kawahara A.、Yamagata K.、Nozaki N.、 Kimura H.
    • Journal Title

      Scientific reports

      Volume: 3 Pages: 2436

    • DOI

      10.1038/srep02436

    • Peer Reviewed
  • [Journal Article] Human inactive X chromosome is compacted through a polycomb-independent SMCHD1-HBiX1 pathway2013

    • Author(s)
      Nozawa R.S.、Nagao K.、Igami K.T.、Shibata S.、Shirai N.、 Nozaki N.、 Sado T.、 Kimura H.、 Obuse C.
    • Journal Title

      Nature Structural and Molecular Biology

      Volume: 20(5) Pages: 566-573

    • DOI

      10.1038/nsmb.2532

    • Peer Reviewed
  • [Journal Article] Vertebrate Spt2 is a novel nucleolar histone chaperone that assists in ribosomal DNA transcription2013

    • Author(s)
      Osakabe A.、 Tachiwana H.、Takaku M.、Hori T.、Obuse C.、 Kimura H.、Fukagawa T.、 Kurumizaka H.
    • Journal Title

      Journal of cell science

      Volume: 126(6) Pages: 1323-1332

    • DOI

      10.1242/jcs.112623

    • Peer Reviewed
  • [Journal Article] A co-localization model of paired ChIP-seq data using a large ENCODE data set enables comparison of multiple samples2013

    • Author(s)
      Maehara K.、Odawara J.、Harada A.、Yoshimi T.、Nagao K.、Obuse C.、Akashi K.、 Tachibana T.、Sakata T.、 Ohkawa Y.
    • Journal Title

      Nucleic acids research

      Volume: 41(1) Pages: 54-62

    • DOI

      10.1093/nar/gks1010

    • Peer Reviewed
  • [Journal Article] Histone chaperone activity of Fanconi anemia proteins, FANCD2 and FANCI, is required for DNA crosslink repair2012

    • Author(s)
      Sato K.、Ishiai M.、Toda K.、Furukoshi S.、 Osakabe A.、Tachiwana H.、Takizawa Y.、 Kagawa W.、Kitao H.、Dohmae N.、Obuse C.、 Kimura H.、Takata M.、Kurumizaka H.
    • Journal Title

      The EMBO journal

      Volume: 31(17) Pages: 3524-3536

    • DOI

      10.1038/emboj.2012.197

    • Peer Reviewed
  • [Journal Article] CDK promotes interactions of Sld3 and Drc1 with Cut5 for initiation of DNA replication in fission yeast2011

    • Author(s)
      Fukuura M.、 Nagao K.、 Obuse C.、 Takahashi T.S.、 Nakagawa T.、Masukata H.
    • Journal Title

      Molecular biology of the cell

      Volume: 22(14) Pages: 2620-2633

    • DOI

      10.1091/mbc.E10-12-0995

    • Peer Reviewed
  • [Journal Article] SHP2 tyrosine phosphatase converts parafibromin/Cdc73 from a tumor suppressor to an oncogenic driver2011

    • Author(s)
      Takahashi A.、Tsutsumi R.、Kikuchi I.、 Obuse C.、Saito Y.、Seidi A.、Karisch R.、 Fernandez M.、Cho T.、Ohnishi N.、 Rozenblatt-Rosen O.、Meyerson M.、Neel B.G.、Hatakeyama M.
    • Journal Title

      Molecular cell

      Volume: 43(1) Pages: 45-56

    • DOI

      10.1016/j.molcel.2011.05.014

    • Peer Reviewed
  • [Journal Article] Sld7, an Sld3-associated protein required for efficient chromosomal DNA replication in budding yeas2011

    • Author(s)
      Tanaka T.、Umemori T.、Endo S.、Muramatsu S.、Kanemaki M.、Kamimura Y.、Obuse C.、Araki H.
    • Journal Title

      The EMBO journal

      Volume: 30(10) Pages: 2019-2030

    • DOI

      10.1038/emboj.2011.115

    • Peer Reviewed
  • [Journal Article] The initial phase of chromosome condensation requires Cdk1-mediated phosphorylation of the CAP-D3 subunit of condensin II2011

    • Author(s)
      Abe S.、Nagasaka K.、Hirayama Y.、Kozuka-Hata H.、Oyama M.、Aoyagi Y.、Obuse C.、Hirota T.
    • Journal Title

      Genes & development

      Volume: 25(8) Pages: 863-874

    • DOI

      10.1101/gad.2016411

    • Peer Reviewed
  • [Journal Article] Human POGZ modulates dissociation of HP1alpha from mitotic chromosome arms through Aurora B activation2010

    • Author(s)
      Nozawa R.S.、Nagao K.、Masuda H.T.、Iwasaki O.、Hirota T.、Nozaki N.、Kimura H.、Obuse C.
    • Journal Title

      Nature cell biology

      Volume: 12(7) Pages: 719-727

    • DOI

      10.1038/ncb2075

    • Peer Reviewed
  • [Journal Article] Casein kinase 2-dependent phosphorylation of human Rad9 mediates the interaction between human Rad9-Hus1-Rad1 complex and TopBP12010

    • Author(s)
      Takeishi Y.、Ohashi E.、Ogawa K.、Masai H.、Obuse C,、Tsurimoto T.
    • Journal Title

      Genes to cells : devoted to molecular & cellular mechanisms

      Volume: 15(7) Pages: 761-771

    • DOI

      10.1111/j.1365-2443.2010.01418.x

    • Peer Reviewed
  • [Journal Article] Nucleosome formation activity of human somatic nuclear autoantigenic sperm protein (sNASP)2010

    • Author(s)
      Osakabe A.、Tachiwana H.、Matsunaga T.、Shiga T.、Nozawa R.S.、Obuse C.、Kurumizaka H.
    • Journal Title

      The Journal of biological chemistry

      Volume: 285(16) Pages: 11913-11921

    • DOI

      10.1074/jbc.M109.083238

    • Peer Reviewed
  • [Journal Article] Cul8/Rtt101 forms a variety of protein complexes that regulate DNA damage response and transcriptional silencing2010

    • Author(s)
      Mimura S.、Yamaguchi T.、Ishii S.、Noro E.、Katsura T.、Obuse C.、Kamura T.
    • Journal Title

      The Journal of biological chemistry

      Volume: 285(13) Pages: 9858-9867

    • DOI

      10.1074/jbc.M109.082107

    • Peer Reviewed
  • [Journal Article] Inner centromere formation requires hMis14, a trident kinetochore protein that specifically recruits HP1 to human chromosomes2010

    • Author(s)
      Kiyomitsu T.、Iwasaki O.、Obuse C.、Yanagida M.
    • Journal Title

      The Journal of cell biology

      Volume: 188(6) Pages: 791-807

    • DOI

      10.1083/jbc.200908096

    • Peer Reviewed
  • [Journal Article] Active establishment of centromeric CENP-A chromatin by RSF complex2009

    • Author(s)
      Perpelescu M.、Nozaki N.、Obuse C.、Yang H.、Yoda K.
    • Journal Title

      The Journal of cell biology

      Volume: 185(3) Pages: 397-407

    • DOI

      10.1083/jcb200903088

    • Peer Reviewed
  • [Journal Article] The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells2009

    • Author(s)
      Uehara R.、Nozawa R.S.、Tomioka A.、Petry S.、Vale RD.、Obuse C.、Goshima G.
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 106(17) Pages: 6998-7003

    • DOI

      10.1073/pnas.0901587106

    • Peer Reviewed
  • [Journal Article] Involvement of human ORC and TRF2 in pre-replication complex assembly at telomeres2008

    • Author(s)
      Tatsumi Y.、Ezura K.、YoshidaK.、Yugawa T.、Narisawa-Saito M.、Kiyono T.、Ohta S.、Obuse C.、Fujita M.
    • Journal Title

      Genes to cells : devoted to molecular & cellular mechanisms

      Volume: 13(10) Pages: 1045-1059

    • DOI

      10.1111/j.1365-2443.2008.01224.x

    • Peer Reviewed
  • [Journal Article] Diminishing HDACs by drugs or mutations promotes normal or abnormal sister chromatid separation by affecting APC/C and adherin2008

    • Author(s)
      Kimata Y.、Matsuyama A.、Nagao K.、Furuya K.、 Obuse C.、Yoshida M.、Yanagida M.
    • Journal Title

      Journal of cell science

      Volume: 121(7) Pages: 1107-1118

    • DOI

      10.1242/jcs.024224

    • Peer Reviewed
  • [Presentation] Human inactive X chromosome is compacted through a Polycomb-independent MACHD1-HBIX1 pathway governed by XIST RNA2013

    • Author(s)
      Obuse C
    • Organizer
      23rd Wilhelm Bernhard Workshop on the cell nucleus
    • Place of Presentation
      Debrecen、Hungary
    • Year and Date
      20130819-24
  • [Presentation] ミクロなクロマチン研究で解くマクロなエピジェネティクス研究2013

    • Author(s)
      小布施 力史
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜 神奈川県
    • Year and Date
      2013-09-12
  • [Presentation] プロテオミクス,ゲノミクスによるエピジェネティクスの機能階層構造の解明2013

    • Author(s)
      小布施 力史
    • Organizer
      第60回日本実験動物学会
    • Place of Presentation
      つくば国際会議場、茨城県
    • Year and Date
      2013-05-16
  • [Presentation] SETDB2-SENP7 complex recognizes histone mark and methylated DNA to maintain H3K9me3 and HP1 on specific loci2012

    • Author(s)
      Nozawa R.S.、Okada T.、Nagao K.、Emiko N.、Shibata S.、Shirai N.、Fujioka A.、Kimura H.、Obuse C.
    • Organizer
      Cold Spring Harbor Laboratory 2012 Meeting Epigenetics and Chromatin
    • Place of Presentation
      NY、USA
    • Year and Date
      2012-09-15
  • [Presentation] HP1 binding proteins facilitate 53BP1 recruitment to damages DNA in human cells2012

    • Author(s)
      Isobe S.Y.、Nozawa R.S.、Shirai N.、Nagao K.、Kimura H.、Obuse C.
    • Organizer
      Cold Spring Harbor Laboratory 2012 Meeting Epigenetics and Chromatin
    • Place of Presentation
      NY、USA
    • Year and Date
      2012-09-15
  • [Presentation] Human inactive X chromosome is compacted through a polycomb-independent SMCHD1-HBIX1 pathway governed by XIST RNA2012

    • Author(s)
      Nozawa R.S.、Nagao K.、Igami K.T.、Shibata S.、Sado T.、Kimura H.、Obuse C.
    • Organizer
      Cold Spring Harbor Laboratory 2012 Meeting Epigenetics and Chromatin
    • Place of Presentation
      NY、USA
    • Year and Date
      2012-09-15
  • [Presentation] タンパク質複合体の構成因子を同定するための試料調整法2012

    • Author(s)
      長尾恒治、野澤竜介、小布施力史
    • Organizer
      日本プロテオーム学会2012年大会
    • Place of Presentation
      日本化学未来館、東京都
    • Year and Date
      2012-07-26
  • [Presentation] 不活性X染色体における非コードRNA XISTを介したヘテロクロマチン形成機構2012

    • Author(s)
      小布施 力史
    • Organizer
      第14回日本RNA 学会年会
    • Place of Presentation
      東北大学百周年記念会館 川内萩ホール、仙台市
    • Year and Date
      2012-07-18
  • [Presentation] 不活性X 染色体における構成的ヘテロクロマチンと条件的ヘテロクロマチンとのクロストーク2012

    • Author(s)
      小布施 力史
    • Organizer
      第6回日本エピジェネティクス研究会年会
    • Place of Presentation
      学術総合センター、東京都
    • Year and Date
      2012-05-14
  • [Presentation] プロテオミクスを用いたエピジェネティクス形成制御ネットワークの解明2011

    • Author(s)
      小布施 力史
    • Organizer
      日本プロテオーム学会2011年会
    • Place of Presentation
      朱鷺メッセ、新潟市
    • Year and Date
      20110728-30
  • [Presentation] POGZ regulate Aurora B kinase activation through binding HP1 on early mitotic chromosome arms2011

    • Author(s)
      Nozawa R.S.
    • Organizer
      International Symposium Physicochemical Field for Genetic Activities
    • Place of Presentation
      淡路市
    • Year and Date
      20110123-26
  • [Presentation] 不活性X 染色体における構成的ヘテロクロマチンと条件的ヘテロクロマチンとのクロストーク2011

    • Author(s)
      小布施 力史
    • Organizer
      京都大学 生命科学セミナー
    • Place of Presentation
      京都大学、京都市
    • Year and Date
      2011-11-28
  • [Presentation] POGZ modulates HP1 dissociation from mitotic chromosome arms for correct activation of Aurora B kinase in human cells2010

    • Author(s)
      Nozawa R.S.、Nagao K.、Matsuda H.T. Kimura H.、Obuse C.
    • Organizer
      75th Symposium : Nuclear Organization & Function
    • Place of Presentation
      NY、USA
    • Year and Date
      20100602-07
  • [Presentation] An HP1-interacting ZINC-FINGER protein POGOZ/ZNF280E is involved in mitotic chromosome segregation2009

    • Author(s)
      Nozawa .R.S., Nagao, K., Obuse, C.
    • Organizer
      Keyston Symposia "Chromatin Dynamics and Higher Order Organization
    • Place of Presentation
      Idaho
    • Year and Date
      20090225-0302
  • [Presentation] An HP1 binding protein POGZ has pivotal role in mitotic chromosome organization in human cells2009

    • Author(s)
      Obuse C.
    • Organizer
      International mini-symposium on chromosome biology : Centromeres to telomeres and the chromatin between
    • Place of Presentation
      札幌市
    • Year and Date
      2009-11-05
  • [Presentation] タンパク質複合体解析のための新技術2008

    • Author(s)
      小布施 力史
    • Organizer
      17th Meeting of Methods in Protein Structure Analysis(MPSA2008)
    • Place of Presentation
      札幌市
    • Year and Date
      2008-08-26
  • [Book] 実験医学、バー小体の正体SMCHD1-HBiX1 複合体によるヒト不活性化X 染色体の凝縮、31(11)2013

    • Author(s)
      長尾 恒治、野澤 竜介、小布施 力史
    • Total Pages
      1771-1775
    • Publisher
      羊土社
  • [Book] 生化学、ヘテロクロマチンタンパク質による Aurora B キナーゼ複合体の局在と活性化のメカニズム、84(2)2012

    • Author(s)
      野澤 竜介、小布施 力史
    • Total Pages
      129-133
    • Publisher
      公益社団法人日本生化学会
  • [Book] 実験医学、ヒトHP1 結合タンパク質のプロテオーム解析からみえてきたHP1 の新機能― HP1 はPOGZ と協調してAurora B キナーゼの活性化に寄与する、28(18)2010

    • Author(s)
      野澤 竜介、長尾 恒治、小布施 力史
    • Total Pages
      2983-2987
    • Publisher
      羊土社
  • [Book] 細胞工学、ヒトHP1 結合タンパク質POGZ は分裂前期のAurora B キナーゼの染色体腕部での活性化に寄与する (特集 細胞分裂・染色体分配の新常識--鍵を握る動原体の制御)、29(9)2010

    • Author(s)
      野澤 竜介、 長尾 恒治、小布施 力史
    • Total Pages
      868-870
    • Publisher
      秀潤社
  • [Book] 実験医学、ヘテロクロマチン結合因子とその機能細胞核―遺伝情報制御と疾患―、272009

    • Author(s)
      野澤 竜介、小布施 力史
    • Total Pages
      123-130
    • Publisher
      羊土社
  • [Book] 細胞工学、染色体を構築する複合体のプロテオミクス解析-明日を拓く新次元プロテオミクス-2009

    • Author(s)
      長尾恒治、野澤竜介、小布施力史
    • Total Pages
      81-90
    • Publisher
      秀潤社
  • [Book] 蛋白質核酸酵素、ブリンキン--紡錘体形成チェックポイントと微小管結合に必須なキネトコア蛋白質 (染色体サイクル--ゲノムの恒常性維持,継承とダイナミクス) -- (染色体の分配とM 期制御)、54(4)2009

    • Author(s)
      清光 智美, 小布施 力史, 柳田 充弘
    • Total Pages
      421-426
    • Publisher
      共立出版
  • [Remarks] 1.北海道新聞、縮むX 染色体仕組み解明-タンパク質結合 不活性化-2013年4月1日 ○ 報道関連・アウトリーチ活動情報](計4件) 新聞報道

  • [Remarks] 2.北海道医療新聞、バー小体の仕組み解明-筋ジス、がん治療に道-2013年4月5日 ○ 報道関連・アウトリーチ活動情報](計4件) 新聞報道

  • [Remarks] 3.毎日新聞(全国版)、X 染色体不活性の仕組み発見、2013年5月30日 ○ 報道関連・アウトリーチ活動情報](計4件) 新聞報道

  • [Remarks] 1.北海道医療新聞、4回連載「女性の"働かない"X 染色体の仕組み」2013年8月23日、8月30日、9月13日、9月20日 ○ 報道関連・アウトリーチ活動情報](計4件) その他の成果発表

  • [Remarks]

    • URL

      http://altair.sci.hokudai.ac.jp/infgen/

URL: 

Published: 2015-07-16  

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