2013 Fiscal Year Final Research Report
Elucidation of molecular basis of the field for gene expression by proteomics
| Project Area | The physicochemical field for genetic activities |
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| Project/Area Number |
20114006
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Hokkaido University |
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Principal Investigator |
OBUSE Chikashi 北海道大学, 先端生命科学研究科(研究院), 教授 (00273855)
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| Co-Investigator(Kenkyū-buntansha) |
NAGAO Koji 北海道大学, 大学院先端生命科学研究院, 講師 (60426575)
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| Project Period (FY) |
2008-11-13 – 2013-03-31
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| Keywords | 染色体 / ヘテロクロマチン / エピジェネティクス / 非コードRNA / ヒストン / HP1 / Aurora B |
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| Research Abstract |
Heterochromatin Protein 1 (HP1) is thought to play a role in compacted heterochromatin formation, through binding to K9 trimethylated histone H3 and its interacting proteins. By proteomic analysis, we identified 82 HP1 binding proteins (HPBPs) in human cells. In addition to known HPBPs, several uncharacterized and/or unexpected proteins were identified [Nature Cell Biol. 12: 719, 2010]. Interestingly, an identified HP1 binding protein was enriched in inactive X chromosomes (Xi), in association with SMCHD1, and thus we named it HBiX1 (HP1 binding protein enriched in Xi). Cytological and epigenomic analyses revealed that HBiX1 and SMCHD1 mediate the compaction of Xi to form heterochromatin structure, by linking the H3K9me3 domains and the XIST/H3K27me3 domains [Nature Struct. & Mol. Biol. 20: 566, 2013]. These studies uncovered that new molecular insights of heterochromatin, especially, how epigenetic marks are translated into higher order chromatin structure to repress transcription.
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[Remarks] 1.北海道新聞、縮むX 染色体仕組み解明-タンパク質結合 不活性化-2013年4月1日 ○ 報道関連・アウトリーチ活動情報](計4件) 新聞報道
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[Remarks] 2.北海道医療新聞、バー小体の仕組み解明-筋ジス、がん治療に道-2013年4月5日 ○ 報道関連・アウトリーチ活動情報](計4件) 新聞報道
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[Remarks] 3.毎日新聞(全国版)、X 染色体不活性の仕組み発見、2013年5月30日 ○ 報道関連・アウトリーチ活動情報](計4件) 新聞報道
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[Remarks] 1.北海道医療新聞、4回連載「女性の"働かない"X 染色体の仕組み」2013年8月23日、8月30日、9月13日、9月20日 ○ 報道関連・アウトリーチ活動情報](計4件) その他の成果発表
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