A study on the molecular and neural mechanisms of chemotaxis and associative learning

2012 Fiscal Year Final Research Report

• Project Area: Systems molecular ethology to understand the operating principle of the nervous system
• Project/Area Number: 20115002
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: IINO Yuichi 東京大学, 大学院・理学系研究科, 教授 (40192471)
• Co-Investigator(Renkei-kenkyūsha): KUNITOMO Hirodfumi 東京大学, 大学院・理学系研究科, 助教 (20302812) ; TOMIOKA Masahiro 東京大学, 大学院・理学系研究科, 助教 (40466800)
• Project Period (FY): 2008 – 2012
• Keywords: 脳・神経 / 神経科学 / 行動学 / 線虫 / 化学走性

Research Abstract

In this study we aimed at understanding the mechanisms of chemotaxis and associative learning in the nematode C. elegans. By quantification of behavior, two mechanisms of chemotaxis were found. On the other hand, mutant isolation revealed that insulin/PI 3-kinase pathway and Gq/phospholipase C/diacylglycerol pathways are important for associative learning of salt concentration and availability of food. Further, changes in the neural circuits that generate the behavioral plasticity was identified.

Research Products

• [Journal Article] Odour concentration-dependent olfactory preference change in C. elegans (2012)
  • Author(s): Yoshida, K., Hirotsu, T., Tagawa, T., Oda, S., Wakabayashi, T., Iino, Y., and Ishihara, T
  • Journal Title: Nat Commun Volume: 3 Pages: 739
  • DOI: DOI:10.1038/ncomms1750
  • Peer Reviewed
• [Journal Article] Mutations in the pqe-1 gene enhance transgene expression in Caenorhabditis elegans (2012)
  • Author(s): Yamada, K., Tsuchiya, J., and Iino, Y
  • Journal Title: G3 (Bethesda) Volume: 2 Pages: 741-751
  • DOI: DOI:10.1534/g3.112.002832
  • Peer Reviewed
• [Journal Article] Temporally-regulated quick activation and inactivation of Ras is important for olfactory behaviour (2012)
  • Author(s): Uozumi, T., Hirotsu, T., Yoshida, K., Yamada, R., Suzuki, A., Taniguchi, G., Iino, Y., and Ishihara, T
  • Journal Title: Sci Rep Volume: 2 Pages: 500
  • DOI: DOI:10.1038/srep00500
  • Peer Reviewed
• [Journal Article] The temporal pattern of stimulation determines the extent and duration of MAPK activation in a Caenorhabditis elegans sensory neuron (2012)
  • Author(s): Tomida, T., Oda, S., Takekawa, M., Iino, Y., and Saito, H
  • Journal Title: Sci Signal Volume: 5 Pages: ra76
  • DOI: DOI:10.1126/scisignal.2002983
  • Peer Reviewed
• [Journal Article] Behavioral Choice between Conflicting Alternatives Is Regulated by a Receptor Guanylyl Cyclase, GCY-28, and a Receptor Tyrosine Kinase, SCD-2, in AIA Interneurons of Caenorhabditis elegans (2011)
  • Author(s): Shinkai, Y., Yamamoto, Y., Fujiwara, M., Tabata, T., Murayama, T., Hirotsu, T., Ikeda, D.D., Tsunozaki, M., Iino, Y., Bargmann, C.I., et al.
  • Journal Title: J Neurosci Volume: 31 Pages: 3007-3015
  • DOI: DOI:10.1523/JNEUROSCI.4691-10.2011
  • Peer Reviewed
• [Journal Article] Neuronal plasticity regulated by the insulin-like signaling pathway underlies salt chemotaxis learning in Caenorhabditis elegans (2011)
  • Author(s): Oda, S., Tomioka, M., and Iino, Y
  • Journal Title: J Neurophysiol Volume: 106 Pages: 301-308
  • DOI: DOI:10.1152/jn.01029.2010
  • Peer Reviewed
• [Journal Article] Roles for class IIA phosphatidylinositol transfer protein in neurotransmission and behavioral plasticity at the sensory neuron synapses of Caenorhabditis elegans (2011)
  • Author(s): Iwata, R., Oda, S., Kunitomo, H., and Iino, Y
  • Journal Title: Proc Natl Acad Sci U S A Volume: 108 Pages: 7589-7594
  • DOI: DOI:10.1073/pnas.1016232108
  • Peer Reviewed
• [Journal Article] A Seven-Transmembrane Receptor That Mediates Avoidance Response to Dihydrocaffeic Acid, a Water-Soluble Repellent in Caenorhabditis elegans (2011)
  • Author(s): Aoki, R., Yagami, T., Sasakura, H., Ogura, K.I., Kajihara, Y., Ibi, M., Miyamae, T., Nakamura, F., Asakura, T., Kanai, Y., et al.
  • Journal Title: J Neurosci Volume: 31 Pages: 16603-16610
  • DOI: DOI:10.1523/JNEUROSCI.4018-11.2011
  • Peer Reviewed
• [Journal Article] Olfactory plasticity is regulated by pheromonal signaling in Caenorhabditis elegans (2010)
  • Author(s): Yamada, K., Hirotsu, T., Matsuki, M., Butcher, R.A., Tomioka, M., Ishihara, T., Clardy, J., Kunitomo, H., and Iino, Y
  • Journal Title: Science Volume: 329 Pages: 1647-1650
  • DOI: DOI:10.1126/science.1192020
  • Peer Reviewed
• [Journal Article] Single-cell transcriptional analysis of taste sensory neuron pair in Caenorhabditis elegans (2010)
  • Author(s): Takayama, J., Faumont, S., Kunitomo, H., Lockery, S.R., and Iino, Y
  • Journal Title: Nucleic Acids Res Volume: 38 Pages: 131-142
  • DOI: DOI:10.1016/j.jtbi.2010.08.020
  • Peer Reviewed
• [Journal Article] Long-tail behavior in locomotion of Caenorhabditis elegans (2010)
  • Author(s): Ohkubo, J., Yoshida, K., Iino, Y., and Masuda, N
  • Journal Title: J Theor Biol Volume: 267 Pages: 213-222
  • DOI: DOI:10.1016/j.jtbi.2010.08.020
  • Peer Reviewed
• [Journal Article] Insulin signaling plays a dual role in Caenorhabditis elegans memory acquisition and memory retrieval (2010)
  • Author(s): Lin, C.H., Tomioka, M., Pereira, S., Sellings, L., Iino, Y., and van der Kooy, D
  • Journal Title: J Neurosci Volume: 30 Pages: 8001-8011
  • DOI: DOI:10.1523/JNEUROSCI.4636-09.2010
  • Peer Reviewed
• [Journal Article] Identification of tubulin deglutamylase among Caenorhabditis elegans and mammalian cytosolic carboxypeptidases (CCPs) (2010)
  • Author(s): Kimura, Y., Kurabe, N., Ikegami, K., Tsutsumi, K., Konishi, Y., Kaplan, O.I., Kunitomo, H., Iino, Y., Blacque, O.E., and Setou, M
  • Journal Title: J Biol Chem Volume: 285 Pages: 22936-22941
  • DOI: DOI:10.1074/jbc.C110.128280
  • Peer Reviewed
• [Journal Article] Reversal of Salt Preference Is Directed by the Insulin/PI3K and Gq/PKC Signaling in Caenorhabditis elegans (2010)
  • Author(s): Adachi, T., Kunitomo, H., Tomioka, M., Ohno, H., Okochi, Y., Mori, I., and Iino, Y
  • Journal Title: Genetics Volume: 186 Pages: 1309-1319
  • DOI: DOI:10.1534/genetics.110.119768
  • Peer Reviewed
• [Journal Article] GPC-1, a G Protein γ-Subunit, Regulates Olfactory Adaptation in Caenorhabditis elegans (2009)
  • Author(s): Yamada, K., Hirotsu, T., Matsuki, M., Kunitomo, H., and Iino, Y
  • Journal Title: Genetics Volume: 181 Pages: 1347-1357
  • DOI: DOI:10.1534/genetics.108.099002
  • Peer Reviewed
• [Journal Article] Lateralized gustatory behavior of C. elegans is controlled by specific receptor-type guanylyl cyclases (2009)
  • Author(s): Ortiz, C.O., Faumont, S., Takayama, J., Ahmed, H.K., Goldsmith, A.D., Pocock, R., McCormick, K.E., Kunimoto, H., Iino, Y., Lockery, S., et al.
  • Journal Title: Curr Biol Volume: 19 Pages: 996-1004
  • DOI: DOI:10.1016/j.cub.2009.05.043
  • Peer Reviewed
• [Journal Article] Parallel use of two behavioral mechanisms for chemotaxis in Caenorhabditis elegans (2009)
  • Author(s): Iino, Y., and Yoshida, K
  • Journal Title: J Neurosci Volume: 29 Pages: 5370-5380
  • DOI: DOI:10.1523/JNEUROSCI.3633-08.2009
  • Peer Reviewed
• [Journal Article] A trophic role for Wnt-Ror kinase signaling during developmental pruning in Caenorhabditis elegans (2009)
  • Author(s): Hayashi, Y., Hirotsu, T., Iwata, R., Kage-Nakadai, E., Kunitomo, H., Ishihara, T., Iino, Y., and Kubo, T
  • Journal Title: Nat Neurosci Volume: 12 Pages: 981-987
  • DOI: DOI:10.1038/nn.2347
  • Peer Reviewed
• [Journal Article] Memory in Caenorhabditis elegans Is Mediated by NMDA-Type Ionotropic Glutamate Receptors (2008)
  • Author(s): Kano, T., Brockie, P.J., Sassa, T., Fujimoto, H., Kawahara, Y., Iino, Y., Mellem, J.E., Madsen, D.M., Hosono, R., and Maricq, A.V
  • Journal Title: Curr Biol Volume: 18 Pages: 1010-1015
  • DOI: DOI:10.1016/j.cub.2008.05.051
  • Peer Reviewed
• [Journal Article] CASY-1, an ortholog of calsyntenins/alcadeins, is essential for learning in Caenorhabditis elegans (2008)
  • Author(s): Ikeda, D.D., Duan, Y., Matsuki, M., Kunitomo, H., Hutter, H., Hedgecock, E.M., and Iino, Y
  • Journal Title: Proc Natl Acad Sci U S A Volume: 105 Pages: 5260-5265
  • DOI: DOI:10.1073/pnas.0711894105
  • Peer Reviewed
• [Presentation] Optogenetic analysis of the mechanism for sensing chemical gradient in C. elegans (2012)
  • Author(s): Yohsuke Satoh, Hirofumi Kunitomo, Yuichi Iino
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] Neural coding of the homeostatic salt chemotaxis based on salt-concentration memory in C. elegans (2012)
  • Author(s): Kunitomo, Hirofumi, Sato Hirofumi, Satoh Yohsuke, Iino, Yuichi
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-20
• [Presentation] The role of a splicing isoform of insulin receptor in starvation-induced behavioral plasticity in C. elegans (2012)
  • Author(s): Masahiro Tomioka, Hayao Ohno, Shinya Kato, Yasuki Naito, Hirofumi Kunitomo and Yuichi Iino
  • Organizer: 第35回 日本神経科学大会
  • Place of Presentation: 名古屋国際会議場(名古屋市)
  • Year and Date: 2012-09-19
• [Presentation] Optogenetic analysis of the mechanism for sensing chemical gradients (2012)
  • Author(s): Yohsuke Satoh, Hirofumi Kunitomo, Yuichi Iino
  • Organizer: 5th East Asia Worm Meeting
  • Place of Presentation: 台北、Taiwan
  • Year and Date: 2012-06-28
• [Presentation] Roles of diacylglycerol signaling in migration toward preferred salt concentration in C. elegans (2012)
  • Author(s): Hirofumi Kunitomo, Hirofumi Sato, Ryo Iwata, Takeshi Adachi, Hayao Ohno and Yuichi Iino
  • Organizer: International Symposium on Olfaction and Taste (ISOT)
  • Place of Presentation: Stockholm
  • Year and Date: 2012-06-25
• [Presentation] Neuronal mechanisms underlying olfactory preference change depending upon odor concentration (2012)
  • Author(s): Kazushi Yoshida, Takaaki Hirotsu, Takanobu Tagawa, Shigekazu Oda, Tokumitsu Wakabayashi, Yuichi Iino, and Takeshi Ishihara
  • Organizer: XVI International Symposium on Olfaction and Taste (ISOT)
  • Place of Presentation: Stockholm、Sweden
  • Year and Date: 2012-06-25
• [Presentation] Axonal transport of a novel isoform of the Insulin/IGF receptor regulates gustatory plasticity in Caenorhabditis elegans (2012)
  • Author(s): Hayao Ohno, Masahiro Tomioka, Shinya Kato, Yasuki Naito, Hirofumi Kunitomo and Yuichi Iino
  • Organizer: XVI International Symposium on Olfaction and Taste
  • Place of Presentation: ストックホルム、スウェーデン
  • Year and Date: 2012-06-24
• [Presentation] Molecular and neuronal mechanisms for experience-dependent gustatory behavior in Caenorhabditis elegans (2012)
  • Author(s): Yuichi Iino, Hirofumi Kunitomo, Masahiro Tomioka, Hayao Ohno, Hirofumi Sato, Ryo Iwata, Shigekazu Oda, Yohsuke Sato and Takeshi Adachi
  • Organizer: XVI International Symposium on Olfaction and Taste
  • Place of Presentation: ストックホルム、スウェーデン
  • Year and Date: 2012-06-24
• [Presentation] Axonal transport of a novel DAF-2 isoform regulates associative learning (2012)
  • Author(s): Hayao Ohno, Masahiro Tomioka, Shinya Kato, Yasuki Naito, Hirofumi Kunitomo, Yuichi Iino
  • Organizer: EMBO Conference Series C. elegans Neurobiology
  • Place of Presentation: ハイデルベルク、ドイツ
  • Year and Date: 2012-06-15
• [Presentation] A Molecular Machinery that Determines Alternative Splicing Patterns of Insulin Receptor in C. elegans Chemosensory Neurons (2012)
  • Author(s): Masahiro Tomioka, Yasuki Naito, Shinya Kato, Hayao Ohno, Yuichi Iino
  • Organizer: RNA Sciences in Cell and Developmental Biology II
  • Place of Presentation: RIKEN CDB(神戸)
  • Year and Date: 2012-06-11
• [Presentation] 線虫の塩への化学走性を調節する神経回路の同定 (2010)
  • Author(s): 吉田和史、飯野雄一
  • Organizer: 日本味と匂学会第46回大会
  • Place of Presentation: 大阪
  • Year and Date: 2010-10-04
• [Remarks]
  • URL: http://molecular-ethology.biochem.s.u-tokyo.ac.jp/IINO_lab_J.html