Mechanism regulating GSC niche system in Drosophila ovary and testis

2012 Fiscal Year Final Research Report

• Project Area: Regulatory Mechanism of Gamete Stem Cells
• Project/Area Number: 20116002
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: National Institutes of Natural Sciences Okazaki Research Facilities
• Principal Investigator: KOBAYASHI Satoru 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (90225508)
• Co-Investigator(Kenkyū-buntansha): ASAOKA Miho 国立遺伝学研究所, 個体遺伝研究系, 助教 (40370118)
• Co-Investigator(Renkei-kenkyūsha): HAYASHI Yoshiki 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 助教 (30508817) ; SATO Masanao 基礎生物学研究所, 発生遺伝学研究部門, 助教 (20517693) ; KITADATE Yu 基礎生物学研究所, 生殖細胞研究部門, 助教 (10455214) ; ABE Kuniya 独立行政法人理化学研究所, 動物変異動態解析技術開発チーム, チームリーダー (40240915)
• Project Period (FY): 2008 – 2012
• Keywords: 配偶子 / 幹細胞 / 生殖細胞 / ニッチ

Research Abstract

In this study, we have been trying to clarify the mechanisms regulating "GSC Niche System" in Drosophila. We found that 1) heparan sulphate proteoglycans (HSPG) are deeply involved in GSC maintenance by restricting the function of growth factors in a narrow region (Niche field) around "Niche cells", 2) Notch, Sevenless and Egfr signaling are required for proper formation of "Niche cells" in the anterior region of male embryonic gonads, and that 3) maternal Ovo and Sxl are required for germline-specific gene expression and sex determination of primordial germ cells, respectively.

Research Products

• [Journal Article] Egfr signaling controls the size of the stem cell precursor pool in the Drosophila ovary. (2013)
  • Author(s): M. Matsuoka, Y. Hiromi and M. Asaoka
  • Journal Title: Mech. Dev. Volume: 4-5 Pages: 241-251
  • Peer Reviewed
• [Journal Article] Germline stem cells and sex determination in Hydra. (2012)
  • Author(s): C. Nishimiya-Fujisawa and S. Kobayashi
  • Journal Title: Int. J. Dev. Biol. Volume: 56 Pages: 499-508
  • Peer Reviewed
• [Journal Article] Glypicans regulate JAK/STAT signaling and distribution of the Unpaired morphogen. (2012)
  • Author(s): Y. Hayashi, T. R. Sexton, K. Dejima, D. W. Perry, M. Takemura, S. Kobayashi, H. Nakato, and D. A. Harrison
  • Journal Title: Development Volume: 139 Pages: 4162-71
  • Peer Reviewed
• [Journal Article] Expression of β-adrenergic-like octopamine receptors during Drosophila development. (2012)
  • Author(s): Y. Ohhara, Y. Kayashima, Y. Hayashi, S. Kobayashi and K. Yamakawa-Kobayashi
  • Journal Title: Zoological Science Volume: 29 Pages: 83-89
  • Peer Reviewed
• [Journal Article] Drosophila Sex lethal gene initiates female development in germline progenitors. (2011)
  • Author(s): K. Hashiyama, Y. Hayashi and S. Kobayashi
  • Journal Title: Science Volume: 333 Pages: 885-888
  • Peer Reviewed
• [Journal Article] Innexin2 gap junctions in somatic support cells are required for cyst formation and for egg chamber formation in Drosophila. (2011)
  • Author(s): M. Mukai, K. Kato, S. Hira, K. Nakamura, H. Kita and S. Kobayashi
  • Journal Title: Mech. Dev. Volume: 128 Pages: 510-523
  • Peer Reviewed
• [Journal Article] Notch and Egfr signaling act antagonistically to regulate germline stem cell niche formation in Drosophila male embryonic gonads. (2010)
  • Author(s): Y. Kitadate and S. Kobayashi
  • Journal Title: Proc. Natl. Acad. Sci., USA. Volume: 107 Pages: 14241-14246
  • Peer Reviewed
• [Journal Article] Small Peptides Switch the Transcriptional Activity of Shavenbaby During Drosophila Embryogenesis. (2010)
  • Author(s): T. Kondo, S. Plaza, J. Zanet, E. Benrabah, P. Valenti, Y. Hashimoto, S. Kobayashi, F. Payre, Y. Kageyama
  • Journal Title: Science Volume: 39 Pages: 336-339
  • Peer Reviewed
• [Journal Article] Non-molting glossy/shroud encodes a short-chain dehydrogenase/ reductase that functions in the "Black Box" of the ecdysteroid biosynthesis pathway. (2010)
  • Author(s): R. Niwa, K. Ito, T. Namiki, Y. Shimada-Niwa, M. Kiuchi, S. Kawaoka, T. Kayukawa, Y. Banno, Y. Fujimoto, S. Shigenobu, S. Kobayashi, T. Shimada, S. Katsuma, and T. Shinoda
  • Journal Title: Development Volume: 137 Pages: 1991-1999
  • Peer Reviewed
• [Journal Article] ショウジョウバエ配偶子幹細胞とニッチ (2010)
  • Author(s): 浅岡美穂
  • Journal Title: 細胞工学 Volume: 29 Pages: 638-644
• [Journal Article] ショウジョウバエ生殖幹細胞システムにおけるニッチの場構築の分子機構 (2010)
  • Author(s): 林良樹、小林悟、中藤博志
  • Journal Title: 細胞工学 Volume: 29 Pages: 645-651
• [Journal Article] Drosophila glypicans regulate the germline stem cell niche. (2009)
  • Author(s): Y. Hayashi, S. Kobayashi and H. Nakato
  • Journal Title: J. Cell Biol. Volume: 187 Pages: 473-480
  • Peer Reviewed
• [Journal Article] Expression of the apoptosis inducer gene head involution defective in primordial germ cells of the Drosophila embryo requires eiger, p53 and loki function. (2009)
  • Author(s): T. Maezawa, K. Arita, S. Shigenobu and S. Kobayashi
  • Journal Title: Develop, Growth and Differ. Volume: 51 Pages: 453-461
  • Peer Reviewed
• [Journal Article] Expression of genes involved in sumoylation in the Drosophila germline. (2009)
  • Author(s): K. Hashiyama and S. Kobayashi
  • Journal Title: Gene Expression Patterns Volume: 9 Pages: 50-53
  • Peer Reviewed
• [Journal Article] 幹細胞の局在を決めるのは何か (2009)
  • Author(s): 浅岡美穂
  • Journal Title: Surgery Frontier Volume: 16 Pages: 81-84
• [Journal Article] Identification of maternal RNAs encoding transcription factors required for germline-specific gene expression in Drosophila embryos. (2008)
  • Author(s): J. Yatsu, M. Hayashi, M. Mukai, K. Arita, S. Shigenobu and S. Kobayashi
  • Journal Title: Int. J. Dev. Biol. Volume: 52 Pages: 913-923
  • Peer Reviewed
• [Presentation] ショウジョウバエの生殖系列の形成とその雌雄を決定するメカニズム (2012)
  • Author(s): 小林悟
  • Organizer: 日本動物学会 第83回大会
  • Place of Presentation: 大阪大学豊中キャンパス、大阪
  • Year and Date: 20120913-15
• [Presentation] "Mechanism regulating germline sexual identity in Drosophila embryos" Germline, Specification, Sex and Stem Cells (2012)
  • Author(s): S. Kobayashi
  • Organizer: The 58/ 60th NIBB Conference
  • Place of Presentation: Okazaki
  • Year and Date: 20120717-21
• [Presentation] Mechanism regulating sex determination of the germline progenitors in Drosophila embryos (2010)
  • Author(s): K. Hashiyama and S. Kobayashi
  • Organizer: Satellite symposium to the SDB and JSDB joint meeting, Germ Cells
  • Place of Presentation: New Mexico, USA.
  • Year and Date: 2010-08-05
• [Presentation] 「ショウジョウバエにおける配偶子幹細胞の形成を制御するメカニズム」『配偶子幹細胞制御に関する研究の新展開』シンポジウムオーガナイザー (2009)
  • Author(s): 小林悟
  • Organizer: 第80回日本動物学会大会
  • Place of Presentation: 静岡
  • Year and Date: 20090917-20
• [Book] ショウジョウバエの卵子幹細胞、「卵子学」森崇英総編集 (2011)
  • Author(s): 浅岡美穂、小林悟
  • Total Pages: 13-24
  • Publisher: 京都大学学術出版会
• [Remarks] Hashiyama, K., Hayashi, Y. and Kobayashi, S. (2011) Science 333, 885-888.の発表論文に関して、以下のように新聞等で報道された。7.8(2011年月・日)朝日新聞 37面、7.8 中日新聞 1面、7.8 毎日新聞 24面、7.8日本経済新聞 34面、7.8日刊工業新聞 21面、7.8 日経産業新聞 9面、7.8 東海 愛知新聞 1面、7.8 読売新聞 25面、 7.22 科学新聞 1面
• [Remarks] アウトリーチ活動 愛知県内の高校(岡崎高校、時習館高校、一宮高校、岡崎北高校)におけるスーパーサイエンス関連講演会:12 件
• [Remarks] 研究室ホームページ
  • URL: http://www.nibb.ac.jp/sections/developmental_biology/kobayashi/