Investigation of GSC/niche・system in the mouse testis

2010 Fiscal Year Self-evaluation Report

• Project Area: Regulatory Mechanism of Gamete Stem Cells
• Project/Area Number: 20116004
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: National Institute for Basic Biology
• Principal Investigator: YOSHIDA Shosei National Institute for Basic Biology, 生殖細胞研究部門, 教授 (60294138)
• Project Period (FY): 2008-11-13 – 2013-03-31
• Keywords: 配偶子 / 精子 / 幹細胞 / ニッチ / マウス / 精巣

Research Abstract

本研究は、マウスのGSC(配偶子幹細胞)である精子形成幹細胞と、それを制御するニッチ細胞、及びニッチの場の実体を解明することを目的として研究を行っている。独自に開発したライブイメージングやパルス標識法を駆使して、精巣内での生きた幹細胞のふるまいを解明し、ほ乳類精巣で精子形成が継続して進行することを保証するメカニズムに挑戦している。

Research Products

• [Journal Article] Bardwell and David Zarkower : The Mammalian Doublesex Homolog DMRT1 Is a Transcriptional Gatekeeper that Controls the Mitosis versus Meiosis Decision in Male Germ Cells. (2010)
  • Author(s): Clinton K.Matson, Mark W.Murphy, Michael D.Griswold, S.Yoshida, Vivian J.
  • Journal Title: Developmental Cell 19(4) Pages: 612-624
  • Peer Reviewed
• [Journal Article] Mouse germ line stem cells undergo rapid and stochastic turnover. (2010)
  • Author(s): A.Klein, T.Nakagawa, R.Ichikawa, S.Yoshida, B.D.Simons
  • Journal Title: Cell Stem Cell co-corresponding authors 7(2) Pages: 214-224
  • Peer Reviewed
• [Journal Article] Functional Hierarchy and Reversibility within the Murine Spermatogenic Stem Cell Compartment. (2010)
  • Author(s): T.Nakagawa, M.Sharma, Y-i.Nabeshima, R.E.Braun, S.Yoshida
  • Journal Title: Science 328 Pages: 62-67
  • Peer Reviewed
• [Journal Article] Stem cells in mammalian spermatogenesis. (2010)
  • Author(s): S.Yoshida
  • Journal Title: Development Growth and Differentiation 52 Pages: 311-317
  • Peer Reviewed
• [Journal Article] The heterogeneity of spermatogonia is revealed by their topology and expression of marker proteins including the germ cell-specific proteins Nanos2 and Nanos3. (2009)
  • Author(s): H.Suzuki, A.Sada, S.Yoshida, Y.Saga
  • Journal Title: Dev Biol 336 Pages: 222-231
  • Peer Reviewed
• [Presentation] Microenvironmental Control of Stem Cell Population in Mouse Spermatogenesis. (2010)
  • Author(s): K.Hara, Y.Kitadate, H.Enomoto, S.Yoshida
  • Organizer: Cold Spring Harbor Laboratory Meeting on Germ Cells
  • Place of Presentation: Cold Spring Harbor, USA(招待講演)
  • Year and Date: 20101005-20101009
• [Presentation] Spatiotemporal control of the spermatogenic stem cells in the mouse testis-toward the understanding of the stem cell niche. (2010)
  • Author(s): S.Yoshida, R.Sugimoto, Y.Kitadate
  • Organizer: The 2010 Society for the Study of Reproduction Meeting, Minisymposium "Germ Cell Microenvironments"
  • Place of Presentation: Milwaukee, USA(招待講演)
  • Year and Date: 20100730-20100803
• [Presentation] Behaviour and control of undifferentiated spermatogonia in the mouse testis. (2010)
  • Author(s): S.Yoshida
  • Organizer: The 16th European Workshop on Molecular and Cellular Endocrinology of the Testis
  • Place of Presentation: Elba, Italy(招待講演)
  • Year and Date: 20100508-20100512
• [Presentation] Spermatogenic Stem Cell System and its Niche in the Mouse Testis. (2009)
  • Author(s): S.Yoshida
  • Organizer: Keystone Symposium : Stem Cell Niche Interactions
  • Place of Presentation: Whistler, Canada(招待講演)
  • Year and Date: 20090421-20090426
• [Presentation] Control of periodic differentiation and persistence of undifferentiated spermatogonia in the mouse testis. (2008)
  • Author(s): S.Yoshida
  • Organizer: Cold Spring Harbor Laboratory Meeting on Germ Cells
  • Place of Presentation: Cold Spring Harbor, USA(招待講演)
  • Year and Date: 20081001-20081005
• [Book] Stem cell niche system in mouse spermatogenesis.(Stem Cell Biology and Regenerative Medicine Series, "Male Germline Stem Cells : Developmental and Regenerative Potential") (2011)
  • Author(s): S.Yoshida
  • Total Pages: 159-175
  • Publisher: Humana Press
• [Book] Control and Regulation of Stem Cells(Spermatogenic stem cell system in the mouse testis Cold Spring Harbor Laboratory Symposia on Quantitative Biology Series 73) (2008)
  • Author(s): S.Yoshida
  • Total Pages: 25
  • Publisher: Cold Spring Harbor Laboratory Press
• [Book] Germ Line Stem Cells.(Cold Spring Harbor Monograph Series Book on 'Germ Cells')
  • Author(s): A.Spradling, M.T.Fuller, R.E.Braun, S.Yoshida.
  • Publisher: Cold Spring Harbor Laboratory Press, Cold Spring Harbor, in press
• [Remarks] 研究成果ホームページ、プレスリリース
  • URL: http://www.nibb.ac.jp/germcell/
• [Remarks]
  • URL: http://www.nibb.ac.jp/press/100319/100319.html
• [Remarks]
  • URL: http://www.nibb.ac.jp/press/100809/100809.html
• [Remarks]
  • URL: http://www.nibb.ac.jp/gamete-stem-cell/Index.html