Regulatory mechanism for systems generating reactive-oxygen signals

2010 Fiscal Year Self-evaluation Report

• Project Area: Signaling functions of reactive oxygen species
• Project/Area Number: 20117002
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyushu University
• Principal Investigator: SUMIMOTO Hideki Kyushu University, 医学研究院, 教授 (30179303)
• Project Period (FY): 2008 – 2012
• Keywords: 活性酸素 / 蛋白質 / シグナル伝達 / 酵素 / NADPHオキシダーゼ

Research Abstract

活性酸素生成酵素NADPHオキシダーゼ(Nox)は、活性酸素シグナルの形成において重要な役割を果たすと考えられている。本研究は、Noxファミリー(Nox1～Nox5)の活性化制御機構の時間的空間的な全体像を解明するものである。特に、「Nox活性化タンパク質の作用機構」に関する研究をさらに押し進めるとともに、「Nox活性化タンパク質の結合によりNoxに何が起こり、電子伝達がどのようになされ、活性酸素生成に至るのか」また「Noxが細胞内のどこに局在するのか、その局在はどのように変化するのか」という問題に焦点をあてる。

Research Products

• [Journal Article] Polarity proteins Beml and Cdc24 are components of the filamentous fungal NADPH oxidase complex (2011)
  • Author(s): akemoto, D., Kamakura, S., Saikia, S., Becker, Y., Wrenn, R., Tanaka, A., Sumimoto, H., Scott, B.
  • Journal Title: Proc.Natl.Acad.Sci.USA 108 Pages: 2861-2866
  • Peer Reviewed
• [Journal Article] A Conserved Region between the TPR and Activation Domains of p671 0x Participates in Activation of the Phagocyte NADPH Oxidase (2010)
  • Author(s): Maehara, Y., Miyano, K., Yuzawa, S., Akimoto, R., Takeya, R., Sumimoto, H.
  • Journal Title: J.Biol.Chem. 41 Pages: 31435-31445
  • Peer Reviewed
• [Journal Article] A region N-terminal to the tandem SH3 domain of p47^<phox> plays a crucial role in activation of the phagocyte NADPH oxidase (2009)
  • Author(s): Taura, M., Miyano, K., Minakami, R., Kamakura, S., Takeya, R., Sumimoto, H.
  • Journal Title: Biochem J 419 Pages: 329-338
  • Peer Reviewed
• [Journal Article] The mammalian formin MOD 1 is activated through phosphorylation by ROCK and mediates thrombin-induced stress fibre formation in endothelial cells (2008)
  • Author(s): Takeya, R., Taniguchi, K., Narumiya, S., Sumimoto, H.
  • Journal Title: EMBO J 27 Pages: 618-628
  • Peer Reviewed
• [Journal Article] regulation, and evolution of Nox-family NADPH oxidases that produce reactive oxygen species (2008)
  • Author(s): Sumimoto, H.Structure
  • Journal Title: FEBS J 75 Pages: 3249-3277
  • Peer Reviewed
• [Presentation] 生体防御に重要な活性酸素生成酵素NADPHオキシダーゼの活性化機構 (2010)
  • Author(s): 宮野佳, 住本英樹
  • Organizer: 第21回日本生体防御学会学術総会
  • Place of Presentation: 仙台(招待講演)
  • Year and Date: 20100722-20100724
• [Presentation] Interaction of Nox-family oxidases with p22phox and soluble regulatory proteins (2010)
  • Author(s): Sumimoto, H.
  • Organizer: Gordon Research Conference (Nox Family NADPH Oxidases)
  • Place of Presentation: Les Diablerets, the Switzerland[invited speaker]
  • Year and Date: 20100606-20100611
• [Presentation] シンポジウム「外科侵襲下の酸素の役割」外敵侵入に備えるNADPHオキシダーゼ (2009)
  • Author(s): 住本英樹
  • Organizer: 日本外科代謝栄養学会第46回学術集会
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 20090709-20090710
• [Presentation] 感染制御に重要な活性酸素生成型NADPHオキシダーゼ(Nox)の調節機構 (2008)
  • Author(s): 住本英樹
  • Organizer: 第61回日本細菌学会九州支部総会/第45回日本ウイルス学会九州支部総会
  • Place of Presentation: 熊本(招待講演)
  • Year and Date: 20081003-20081004
• [Presentation] 活性酸素生成酵素Noxの調節機構 (2008)
  • Author(s): 住本英樹
  • Organizer: 第61回日本酸化ストレス学会学術集会
  • Place of Presentation: 京都(招待講演)
  • Year and Date: 20080619-20080620
• [Book] In Free Radical Biology in Digestive Diseases(Frontiers of Gastrointestinal Research)Vol.29 (2011)
  • Author(s): Sumimoto, H., Minakami, R., Miyano, K., S.Karger AG
  • Total Pages: 176
• [Book] 再生医学事典 (2009)
  • Author(s): 住本英樹
  • Total Pages: 584
  • Publisher: 朝倉書店炎症
• [Book] 酸化ストレスの医学 (2008)
  • Author(s): 住本英樹, 前原優一, 宮野佳
  • Total Pages: 384
  • Publisher: 診断と治療社